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KRAS elisa kit :: Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog ELISA Kit

Scan QR to view Datasheet Catalog #    MBS936810
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), ELISA Kit

★Popular Item★
 Also Known As   

Human GTPase KRas, KRAS ELISA Kit

 Product Synonym Names    Human GTPase KRas (KRAS) ELISA kit; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RASK2; K-ras p21 protein; Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; PR310 c-K-ras oncogene; c-K-ras2 protein; ; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
 Product Gene Name   

KRAS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS936810 Testing Data    Testing Data PDF
 OMIM    615278
 3D Structure    ModBase 3D Structure for P01116
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human KRAS. No significant cross-reactivity or interference between human KRAS and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates.
 Assay Type    Sandwich
 Detection Range    23.5 pg/ml -1500 pg/ml.
 Sensitivity    5.8 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KRAS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KRAS purchase    MBS936810 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 23.5 pg/ml -1500 pg/ml in biological research samples containing KRAS, with an estimated sensitivity of 5.8 pg/ml. The ELISA analytical biochemical technique of the MBS936810 kit is based on KRAS antibody-KRAS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KRAS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KRAS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for KRAS elisa kit

   Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KRAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRAS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRAS bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of KRAS elisa kit    KRAS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS936810. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KRAS. It may not necessarily be applicable to this product.
 NCBI GI #    15718761
 NCBI GeneID    3845
 NCBI Accession #    NP_004976.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004985.4 [Other Products]
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 UniProt Primary Accession #    P01116 [Other Products]
 UniProt Secondary Accession #    P01118; Q96D10; A8K8Z5; B0LPF9 [Other Products]
 UniProt Related Accession #    P01116 [Other Products]
 Molecular Weight    21,656 Da
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 NCBI Official Full Name    GTPase KRas isoform b
 NCBI Official Synonym Full Names    Kirsten rat sarcoma viral oncogene homolog
 NCBI Official Symbol    KRAS [Similar Products]
 NCBI Official Synonym Symbols   
NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
[Similar Products]
 NCBI Protein Information    GTPase KRas; K-Ras 2; c-Ki-ras; oncogene KRAS2; K-ras p21 protein; c-Kirsten-ras protein; PR310 c-K-ras oncogene; transforming protein p21; cellular c-Ki-ras2 proto-oncogene; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
 UniProt Protein Name    GTPase KRas
 UniProt Synonym Protein Names   
K-Ras 2; Ki-Ras; c-K-ras; c-Ki-rasCleaved into the following chain:GTPase KRas, N-terminally processed
 Protein Family    GTPase
 UniProt Gene Name    KRAS [Similar Products]
 UniProt Synonym Gene Names    KRAS2; RASK2 [Similar Products]
 UniProt Entry Name    RASK_HUMAN
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 NCBI Summary for KRAS    This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
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 UniProt Comments for KRAS    KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; G protein, monomeric; Motility/polarity/chemotaxis; Oncoprotein; G protein, monomeric, Ras

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; mitochondrion; membrane; cytoplasm; plasma membrane; cytosol; lipid raft

Molecular Function: protein binding; GDP binding; GTP binding; GMP binding; protein complex binding; LRR domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; activation of MAPKK activity; nerve growth factor receptor signaling pathway; response to glucocorticoid stimulus; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; small GTPase mediated signal transduction; positive regulation of cell proliferation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of nitric-oxide synthase activity; MAPKKK cascade; response to mineralocorticoid stimulus; social behavior; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; striated muscle cell differentiation; positive regulation of protein amino acid phosphorylation; actin cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration

Disease: Bladder Cancer; Gastric Cancer, Hereditary Diffuse; Pancreatic Cancer; Noonan Syndrome 3; Leukemia, Acute Myeloid; Lung Cancer; Breast Cancer; Schimmelpenning-feuerstein-mims Syndrome; Cardiofaciocutaneous Syndrome 2
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 Research Articles on KRAS    1. KRAS mutation is associated with esophageal squamous cell carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KRAS elisa kitPathways associated with KRAS elisa kit
 Reference Product  PubMed Publications
 RAF1 elisa kit  >44 publications with KRAS and RAF1
 SHOC2 elisa kit  >10 publications with KRAS and SHOC2
 BCL2 elisa kit  >9 publications with KRAS and BCL2
 RASSF2 elisa kit  >5 publications with KRAS and RASSF2
 RALGDS elisa kit  >2 publications with KRAS and RALGDS
 RASSF5 elisa kit  >1 publications with KRAS and RASSF5
 RAP1GDS1 elisa kit  >1 publications with KRAS and RAP1GDS1
 PIK3CG elisa kit  >1 publications with KRAS and PIK3CG
 FNTA elisa kit  >1 publications with KRAS and FNTA
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of RAS In B Cells Pathway antibodies  Activation Of RAS In B Cells Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
Diseases associated with KRAS elisa kitOrgans/Tissues associated with KRAS elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2247 publications with KRAS and Neoplasms
 Carcinoma Antibodies  >1100 publications with KRAS and Carcinoma
 Colorectal Neoplasms Antibodies  >1004 publications with KRAS and Colorectal Neoplasms
 Intestinal Neoplasms Antibodies  >971 publications with KRAS and Intestinal Neoplasms
 Adenocarcinoma Antibodies  >730 publications with KRAS and Adenocarcinoma
 Lung Neoplasms Antibodies  >560 publications with KRAS and Lung Neoplasms
 Pancreatic Neoplasms Antibodies  >261 publications with KRAS and Pancreatic Neoplasms
 Neoplasm Metastasis Antibodies  >253 publications with KRAS and Neoplasm Metastasis
 Colonic Neoplasms Antibodies  >197 publications with KRAS and Colonic Neoplasms
 Adenoma Antibodies  >159 publications with KRAS and Adenoma
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >672 publications with KRAS and Lung
 Blood Antibodies  >197 publications with KRAS and Blood
 Pancreas Antibodies  >134 publications with KRAS and Pancreas
 Liver Antibodies  >133 publications with KRAS and Liver
 Skin Antibodies  >117 publications with KRAS and Skin
 Vascular Antibodies  >91 publications with KRAS and Vascular
 Lymph Antibodies  >79 publications with KRAS and Lymph
 Lymph Node Antibodies  >78 publications with KRAS and Lymph Node
 Intestine Antibodies  >68 publications with KRAS and Intestine
 Heart Antibodies  >66 publications with KRAS and Heart
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