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CRKL elisa kit :: Human v-crk sarcoma virus CT10 oncogene homolog (avian) -like ELISA Kit

Scan QR to view Datasheet Catalog #    MBS901280
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

v-crk sarcoma virus CT10 oncogene homolog (avian) -like (CRKL), ELISA Kit

 Also Known As   

Human Crk-like protein, CRKL ELISA Kit

 Product Synonym Names    Human Crk-like protein (CRKL) ELISA kit; OTTHUMP00000198539; v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog (avian) -like
 Product Gene Name   

CRKL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS901280 Testing Data    Testing Data PDF
 OMIM    602007
 3D Structure    ModBase 3D Structure for P46109
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human CRKL. No significant cross-reactivity or interference between Human CRKL and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml-2000 pg/ml
 Sensitivity    7.81 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of CRKL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CRKL purchase    MBS901280 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the v-crk sarcoma virus CT10 oncogene homolog (avian) -like (CRKL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing CRKL, with an estimated sensitivity of 7.81 pg/ml. The ELISA analytical biochemical technique of the MBS901280 kit is based on CRKL antibody-CRKL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRKL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRKL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CRKL elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRKL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRKL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRKL bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CRKL elisa kit    CRKL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS901280
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NCBI/Uniprot data below describe general gene information for CRKL. It may not necessarily be applicable to this product.
 NCBI GI #    4885153
 NCBI GeneID    1399
 NCBI Accession #    NP_005198.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005207.3 [Other Products]
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 UniProt Primary Accession #    P46109 [Other Products]
 UniProt Secondary Accession #    A8KA44; D3DX35 [Other Products]
 UniProt Related Accession #    P46109 [Other Products]
 Molecular Weight    33,777 Da
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 NCBI Official Full Name    crk-like protein
 NCBI Official Synonym Full Names    v-crk avian sarcoma virus CT10 oncogene homolog-like
 NCBI Official Symbol    CRKL [Similar Products]
 NCBI Protein Information    crk-like protein
 UniProt Protein Name    Crk-like protein
 Protein Family    Crk-like protein
 UniProt Gene Name    CRKL [Similar Products]
 UniProt Entry Name    CRKL_HUMAN
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 NCBI Summary for CRKL    This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]
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 UniProt Comments for CRKL    CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; endosome

Molecular Function: signal transducer activity; protein binding; SH3/SH2 adaptor activity

Biological Process: anterior/posterior pattern formation; blood vessel development; organ morphogenesis; nerve growth factor receptor signaling pathway; thymus development; activation of MAPKK activity; heart development; Ras protein signal transduction; JNK cascade; parathyroid gland development
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 Research Articles on CRKL    1. CrkL is differently expressed in different grades of gliomas, and correlated to WHO grade.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CRKL elisa kitPathways associated with CRKL elisa kit
 Reference Product  PubMed Publications
 BCR elisa kit  >138 publications with CRKL and BCR
 CRK elisa kit  >120 publications with CRKL and CRK
 CBL elisa kit  >65 publications with CRKL and CBL
 GRB2 elisa kit  >47 publications with CRKL and GRB2
 CD34 elisa kit  >24 publications with CRKL and CD34
 LYN elisa kit  >16 publications with CRKL and LYN
 SYK elisa kit  >11 publications with CRKL and SYK
 DAB1 elisa kit  >10 publications with CRKL and DAB1
 BCAR1 elisa kit  >8 publications with CRKL and BCAR1
 SHC1 elisa kit  >8 publications with CRKL and SHC1
 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
Diseases associated with CRKL elisa kitOrgans/Tissues associated with CRKL elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >129 publications with CRKL and Neoplasms
 Leukemia Antibodies  >124 publications with CRKL and Leukemia
 Leukemia, Myelogenous, Chronic, BCR-ABL Positive Antibodies  >79 publications with CRKL and Leukemia, Myelogenous, Chronic, BCR-ABL Positive
 Bone Marrow Diseases Antibodies  >77 publications with CRKL and Bone Marrow Diseases
 Disease Models, Animal Antibodies  >11 publications with CRKL and Disease Models, Animal
 Leukemia, Myeloid, Acute Antibodies  >10 publications with CRKL and Leukemia, Myeloid, Acute
 Craniofacial Abnormalities Antibodies  >8 publications with CRKL and Craniofacial Abnormalities
 DiGeorge Syndrome Antibodies  >8 publications with CRKL and DiGeorge Syndrome
 Neoplasms, Experimental Antibodies  >7 publications with CRKL and Neoplasms, Experimental
 Lymphoma, B-Cell Antibodies  >6 publications with CRKL and Lymphoma, B-Cell
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >99 publications with CRKL and Blood
 Bone Antibodies  >18 publications with CRKL and Bone
 Nerve Antibodies  >16 publications with CRKL and Nerve
 Bone Marrow Antibodies  >16 publications with CRKL and Bone Marrow
 Brain Antibodies  >13 publications with CRKL and Brain
 Lung Antibodies  >10 publications with CRKL and Lung
 Embryonic Tissue Antibodies  >10 publications with CRKL and Embryonic Tissue
 Heart Antibodies  >5 publications with CRKL and Heart
 Thymus Antibodies  >4 publications with CRKL and Thymus
 Parathyroid Antibodies  >4 publications with CRKL and Parathyroid
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