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ERBB2 elisa kit :: Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701521
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), ELISA Kit

★Popular Item★
 Also Known As   

Human epidermal growth factor receptor 2, sp185/HER2 ELISA Kit

 Product Synonym Names    Human epidermal growth factor receptor 2 (sp185/HER2) ELISA Kit; CD340; HER-2; HER-2/neu; HER2; NEU; NGL; TKR1; c-erb B2/neu protein; erbB-2; herstatin; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2; neuro/glioblastoma derived oncogene homolog (avian)
 Product Gene Name   

ERBB2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701521 Testing Data    Testing Data PDF
 OMIM    613659
 3D Structure    ModBase 3D Structure for P04626
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ERBB2. No significant cross-reactivity or interference between Human ERBB2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    0.156 ng/ml-10 ng/ml
 Sensitivity    0.04 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Transcription/Transcription regulation
 Protein Biological Process 3    Transcription
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ERBB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ERBB2 purchase    MBS701521 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml-10 ng/ml in biological research samples containing ERBB2, with an estimated sensitivity of 0.04 ng/ml. The ELISA analytical biochemical technique of the MBS701521 kit is based on ERBB2 antibody-ERBB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ERBB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ERBB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ERBB2 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sp185/HER2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sp185/HER2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sp185/HER2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sp185/HER2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ERBB2 elisa kit    ERBB2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS701521. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ERBB2. It may not necessarily be applicable to this product.
 NCBI GI #    54792098
 NCBI GeneID    2064
 NCBI Accession #    NP_001005862.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001005862.2 [Other Products]
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 UniProt Primary Accession #    P04626 [Other Products]
 UniProt Secondary Accession #    Q14256; Q6LDV1; Q9UMK4; B2RZG3; B4DHN3; X5D2V5 [Other Products]
 UniProt Related Accession #    P04626 [Other Products]
 Molecular Weight    137,910 Da
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 NCBI Official Full Name    receptor tyrosine-protein kinase erbB-2 isoform b
 NCBI Official Synonym Full Names    v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
 NCBI Official Symbol    ERBB2 [Similar Products]
 NCBI Official Synonym Symbols   
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
[Similar Products]
 NCBI Protein Information    receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
 UniProt Protein Name    Receptor tyrosine-protein kinase erbB-2
 UniProt Synonym Protein Names   
Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen: CD340
 Protein Family    Receptor tyrosine-protein kinase
 UniProt Gene Name    ERBB2 [Similar Products]
 UniProt Synonym Gene Names    HER2; MLN19; NEU; NGL; MLN 19 [Similar Products]
 UniProt Entry Name    ERBB2_HUMAN
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 NCBI Summary for ERBB2    This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ERBB2    Function: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.6 PublicationsManual assertion based on experiment in:Ref.12

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 PublicationManual assertion based on experiment in:Ref.40

Enzyme regulation: Activated by dimerization. Not activated by EGF, TGF-alpha and amphiregulin. Interaction with PTK6 increases its intrinsic kinase activity.2 PublicationsManual assertion based on experiment in:Ref.25
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 Research Articles on ERBB2    1. High HER2 expression is associated with gastric carcinomas with advanced stages.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ERBB2 elisa kitPathways associated with ERBB2 elisa kit
 Reference Product  PubMed Publications
 EGFR elisa kit  >2849 publications with ERBB2 and EGFR
 ERBB3 elisa kit  >792 publications with ERBB2 and ERBB3
 SRC elisa kit  >386 publications with ERBB2 and SRC
 GRB2 elisa kit  >109 publications with ERBB2 and GRB2
 GRB7 elisa kit  >79 publications with ERBB2 and GRB7
 ERBB2IP elisa kit  >42 publications with ERBB2 and ERBB2IP
 SHC1 elisa kit  >34 publications with ERBB2 and SHC1
 UBC elisa kit  >3 publications with ERBB2 and UBC
 HSP90AA1 elisa kit  >1 publications with ERBB2 and HSP90AA1
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
Diseases associated with ERBB2 elisa kitOrgans/Tissues associated with ERBB2 elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >12013 publications with ERBB2 and Breast Neoplasms
 Skin Diseases Antibodies  >11172 publications with ERBB2 and Skin Diseases
 Carcinoma Antibodies  >6549 publications with ERBB2 and Carcinoma
 Adenocarcinoma Antibodies  >3435 publications with ERBB2 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >2824 publications with ERBB2 and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >1659 publications with ERBB2 and Neoplasms, Experimental
 Disease Progression Antibodies  >1581 publications with ERBB2 and Disease Progression
 Lymphatic Metastasis Antibodies  >1297 publications with ERBB2 and Lymphatic Metastasis
 Gastrointestinal Neoplasms Antibodies  >1251 publications with ERBB2 and Gastrointestinal Neoplasms
 Lung Neoplasms Antibodies  >1163 publications with ERBB2 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1754 publications with ERBB2 and Blood
 Lymph Node Antibodies  >1489 publications with ERBB2 and Lymph Node
 Lung Antibodies  >1099 publications with ERBB2 and Lung
 Stomach Antibodies  >512 publications with ERBB2 and Stomach
 Brain Antibodies  >474 publications with ERBB2 and Brain
 Prostate Antibodies  >450 publications with ERBB2 and Prostate
 Liver Antibodies  >431 publications with ERBB2 and Liver
 Bone Antibodies  >368 publications with ERBB2 and Bone
 Mammary Gland Antibodies  >349 publications with ERBB2 and Mammary Gland
 Heart Antibodies  >309 publications with ERBB2 and Heart
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