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WNT5B elisa kit :: Human wingless-type MMTV integration site family, member 5B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS941658
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

wingless-type MMTV integration site family, member 5B (WNT5B), ELISA Kit

★Popular Item★
 Also Known As   

Human Protein Wnt-5b, WNT5B ELISA Kit

 Product Synonym Names    Human Protein Wnt-5b (WNT5B) ELISA kit; MGC2648; WNT-5B protein; wingless-type MMTV integration site family; member 5B
 Product Gene Name   

WNT5B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS941658 Testing Data    Testing Data PDF
 OMIM    606361
 3D Structure    ModBase 3D Structure for Q9H1J7
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human WNT5B. No significant cross-reactivity or interference between human WNT5B and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.156 ng/ml -10 ng/ml.
 Sensitivity    The minimum detectable dose of human WNT5B is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of WNT5B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for WNT5B purchase    MBS941658 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the wingless-type MMTV integration site family, member 5B (WNT5B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml -10 ng/ml in biological research samples containing WNT5B, with an estimated sensitivity of The minimum detectable dose of human WNT5B is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS941658 kit is based on WNT5B antibody-WNT5B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT5B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT5B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for WNT5B elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT5B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT5B bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of WNT5B elisa kit    WNT5B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS941658. Click to request current manual
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NCBI/Uniprot data below describe general gene information for WNT5B. It may not necessarily be applicable to this product.
 NCBI GI #    17402919
 NCBI GeneID    81029
 NCBI Accession #    NP_110402.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_030775.2 [Other Products]
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 UniProt Primary Accession #    Q9H1J7 [Other Products]
 UniProt Secondary Accession #    Q96S49; Q9BV04; A8K315; D3DUP9 [Other Products]
 UniProt Related Accession #    Q9H1J7 [Other Products]
 Molecular Weight    40,323 Da
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 NCBI Official Full Name    protein Wnt-5b
 NCBI Official Synonym Full Names    wingless-type MMTV integration site family, member 5B
 NCBI Official Symbol    WNT5B [Similar Products]
 NCBI Protein Information    protein Wnt-5b; WNT-5B protein
 UniProt Protein Name    Protein Wnt-5b
 UniProt Gene Name    WNT5B [Similar Products]
 UniProt Entry Name    WNT5B_HUMAN
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 NCBI Summary for WNT5B    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
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 UniProt Comments for WNT5B    WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; plasma membrane; extracellular region

Molecular Function: frizzled binding; receptor binding

Biological Process: fat cell differentiation; neuron differentiation; cell fate commitment; Wnt receptor signaling pathway; wound healing; chondrocyte differentiation; positive regulation of fat cell differentiation; positive regulation of cell migration
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 Research Articles on WNT5B    1. Ursolic acid suppressed the expression of Wnt5alpha/beta and beta-catenin, inducing apoptosis in prostate cancer cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with WNT5B elisa kitPathways associated with WNT5B elisa kit
 Reference Product  PubMed Publications
 PORCN elisa kit  >1 publications with WNT5B and PORCN
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
Diseases associated with WNT5B elisa kitOrgans/Tissues associated with WNT5B elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >8 publications with WNT5B and Breast Neoplasms
 Skin Diseases Antibodies  >6 publications with WNT5B and Skin Diseases
 Stomach Diseases Antibodies  >4 publications with WNT5B and Stomach Diseases
 Disease Models, Animal Antibodies  >4 publications with WNT5B and Disease Models, Animal
 Fibrosis Antibodies  >3 publications with WNT5B and Fibrosis
 Drug Toxicity Antibodies  >3 publications with WNT5B and Drug Toxicity
 Necrosis Antibodies  >3 publications with WNT5B and Necrosis
 Brain Diseases Antibodies  >3 publications with WNT5B and Brain Diseases
 Heart Defects, Congenital Antibodies  >2 publications with WNT5B and Heart Defects, Congenital
 Liver Diseases Antibodies  >2 publications with WNT5B and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >18 publications with WNT5B and Embryonic Tissue
 Bone Antibodies  >15 publications with WNT5B and Bone
 Muscle Antibodies  >12 publications with WNT5B and Muscle
 Brain Antibodies  >9 publications with WNT5B and Brain
 Lung Antibodies  >8 publications with WNT5B and Lung
 Kidney Antibodies  >8 publications with WNT5B and Kidney
 Eye Antibodies  >7 publications with WNT5B and Eye
 Liver Antibodies  >6 publications with WNT5B and Liver
 Heart Antibodies  >6 publications with WNT5B and Heart
 Intestine Antibodies  >5 publications with WNT5B and Intestine
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