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IL-5 elisa kit :: Interleukin-5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS590010
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Wells  /  $605 +1 FREE 8GB USB
 
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 Product Name   

Interleukin-5 (IL-5), ELISA Kit

 Also Known As   

Interleukin-5 (IL-5)

 Product Gene Name   

IL-5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    147850
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 Specificity    This sandwich ELISA can detect both natural and recombinant human IL-5. This kit exhibits no detectable cross-reactivity with human; GM-CSF, GRO a, GRO b, INF-g, INF a, IL-1a, IL-1b, IL-1 RA, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL- 11, IL-12, IL-13, IL-15, TNF-a, mouse IL-5 and IL-9.
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 Samples    Serum, plasma, cell culture medium, and urine
 Assay Type    Sandwich
 Sensitivity    The minimum detectable quantities of human IL-5 as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 7.5 pg/mL and 6.3 pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities.
 Intra-assay Precision    To determine within-run precision, three different samples of known concentration were assayed by replicates of 20 in 1 assay.
 Inter-assay Precision    To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.
 Intended Uses    This Human Interleukin 5 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 5 (IL-5) concentrations in serum, plasma, cell culture medium, and urine. This kit is intended for LABORATORY RESEARCH ONLY and is not to be used for diagnostic or therapeutic purposes.
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 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-5 purchase    MBS590010 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-5 (IL-5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-5, with an estimated sensitivity of The minimum detectable quantities of human IL-5 as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 7.5 pg/mL and 6.3 pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities. The ELISA analytical biochemical technique of the MBS590010 kit is based on IL-5 antibody-IL-5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture medium, and urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-5 elisa kit

   Principle of the assay: This IL-5 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-5 and incubated. IL-5, if present, will bind and become immobilized by the antibody pre-coated on the wells and then become "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-5 and other components of the sample. In order to quantitatively determine the amount of IL-5 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-5, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.
 Product Categories/Family for IL-5 elisa kit    ELISA Kits for Human Cytokines and Receptors
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 Typical Testing Data/Standard Curve (for reference only) of IL-5 elisa kit    IL-5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS590010. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-5. It may not necessarily be applicable to this product.
 NCBI GI #    4504671
 NCBI GeneID    3567
 NCBI Accession #    NP_000870.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000879.2 [Other Products]
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 UniProt Secondary Accession #    Q13840 [Other Products]
 UniProt Related Accession #    P05113 [Other Products]
 Molecular Weight    15,238 Da
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 NCBI Official Full Name    interleukin-5
 NCBI Official Synonym Full Names    interleukin 5
 NCBI Official Symbol    IL5 [Similar Products]
 NCBI Official Synonym Symbols   
EDF; TRF; IL-5
[Similar Products]
 NCBI Protein Information    interleukin-5; B-cell differentiation factor I; T-cell replacing factor; colony-stimulating factor, eosinophil; eosinophil differentiation factor
 UniProt Protein Name    Interleukin-5
 UniProt Synonym Protein Names   
B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
 Protein Family    Interleukin
 UniProt Gene Name    IL5 [Similar Products]
 UniProt Entry Name    IL5_HUMAN
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 NCBI Summary for IL-5    This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
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 UniProt Comments for IL-5    IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; immune response; positive regulation of transcription factor activity; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation
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 Research Articles on IL-5    1. HDM-specific IL-5 responses at age 3 years or later are the best measure of T cell function for predicting asthma at age 8 years.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-5 elisa kitPathways associated with IL-5 elisa kit
 Reference Product  PubMed Publications
 IL4 elisa kit  >216 publications with IL-5 and IL4
 IL2 elisa kit  >101 publications with IL-5 and IL2
 IL3 elisa kit  >50 publications with IL-5 and IL3
 JUN elisa kit  >37 publications with IL-5 and JUN
 IL9 elisa kit  >25 publications with IL-5 and IL9
 IL5RA elisa kit  >24 publications with IL-5 and IL5RA
 CSF2RB elisa kit  >5 publications with IL-5 and CSF2RB
 SDCBP elisa kit  >1 publications with IL-5 and SDCBP
 FOS elisa kit  >1 publications with IL-5 and FOS
 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
Diseases associated with IL-5 elisa kitOrgans/Tissues associated with IL-5 elisa kit
 Disease Name  Pubmed Publications
 Hypersensitivity Antibodies  >133 publications with IL-5 and Hypersensitivity
 Asthma Antibodies  >114 publications with IL-5 and Asthma
 Inflammation Antibodies  >111 publications with IL-5 and Inflammation
 Lung Diseases Antibodies  >88 publications with IL-5 and Lung Diseases
 Eosinophilia Antibodies  >71 publications with IL-5 and Eosinophilia
 Necrosis Antibodies  >61 publications with IL-5 and Necrosis
 Bronchial Hyperreactivity Antibodies  >13 publications with IL-5 and Bronchial Hyperreactivity
 Fibrosis Antibodies  >13 publications with IL-5 and Fibrosis
 Hyperplasia Antibodies  >10 publications with IL-5 and Hyperplasia
 Dermatitis, Atopic Antibodies  >9 publications with IL-5 and Dermatitis, Atopic
 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >8 publications with IL-5 and Thymus
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