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IL-6 elisa kit :: Interleukin-6 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS590011
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Wells  /  $605 +1 FREE 8GB USB
 
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 Product Name   

Interleukin-6 (IL-6), ELISA Kit

 Also Known As   

Interleukin-6 (IL-6)

 Product Gene Name   

IL-6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    147620
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 Specificity    This sandwich ELISA recognizes both natural and recombinant human IL-6. This kit exhibits no detectable cross-reactivity with human; SAA, EGF, IL-8, IL-16, MCAF, MCP-3, TNF-?, M-CSF, GM-CSF, TGF-beta1, RANTES, FGF, MIP-1alpha, CRP, EPO, IFN-gamma.
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 Samples    Cell Culture Supernatant, serum, plasma, and other biological fluids
 Assay Type    Sandwich
 Sensitivity    The minimum detectable dose of IL-6 was determined by adding two standard deviations to the mean optical density value of the zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 2.0 pg/mL and using Calibrator Diluent II is 1.75 pg/mL.
 Intra-assay Precision    To determine within-run precision, three different samples of known concentration were assayed by replicates of 20 in 1 assay.
 Inter-assay Precision    To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.
 Intended Uses    This Human IL-6 ELISA kit is to be used for the in vitro quantitative determination of human interleukin-6 (IL-6) concentrations in cell culture supernatant, serum, plasma, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.
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 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-6 purchase    MBS590011 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-6 (IL-6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-6, with an estimated sensitivity of The minimum detectable dose of IL-6 was determined by adding two standard deviations to the mean optical density value of the zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 2.0 pg/mL and using Calibrator Diluent II is 1.75 pg/mL. The ELISA analytical biochemical technique of the MBS590011 kit is based on IL-6 antibody-IL-6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatant, serum, plasma, and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-6 elisa kit

   Principle of the assay: This IL-6 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-6 and incubated. IL-6, if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-6 and other components of the sample. In order to quantitatively determine the amount of IL-6 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm +/- 2 nm. In order to measure the concentration of IL-6 in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-6 concentration (pg/mL). The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for IL-6 elisa kit    ELISA Kits for Human Cytokines and Receptors
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 Typical Testing Data/Standard Curve (for reference only) of IL-6 elisa kit    IL-6 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS590011. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-6. It may not necessarily be applicable to this product.
 NCBI GI #    10834984
 NCBI GeneID    3569
 NCBI Accession #    NP_000591.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000600.3 [Other Products]
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 UniProt Secondary Accession #    Q9UCU2; Q9UCU3; Q9UCU4 [Other Products]
 UniProt Related Accession #    P05231 [Other Products]
 Molecular Weight    23,718 Da
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 NCBI Official Full Name    interleukin-6
 NCBI Official Synonym Full Names    interleukin 6
 NCBI Official Symbol    IL6 [Similar Products]
 NCBI Official Synonym Symbols   
HGF; HSF; BSF2; IL-6; IFNB2
[Similar Products]
 NCBI Protein Information    interleukin-6; B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; IFN-beta-2; hybridoma growth factor; interferon beta-2; interferon, beta 2; interleukin BSF-2
 UniProt Protein Name    Interleukin-6
 UniProt Synonym Protein Names   
B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
 Protein Family    Interleukin
 UniProt Gene Name    IL6 [Similar Products]
 UniProt Entry Name    IL6_HUMAN
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 NCBI Summary for IL-6    This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
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 UniProt Comments for IL-6    IL6: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic- onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Belongs to the IL-6 superfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p21

Cellular Component: extracellular space; cytoplasm; extracellular region; interleukin-6 receptor complex; external side of plasma membrane

Molecular Function: protein binding; interleukin-6 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of collagen biosynthetic process; negative regulation of cytokine secretion; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; positive regulation of leukocyte chemotaxis; glucose homeostasis; bone remodeling; activation of NF-kappaB transcription factor; response to caffeine; positive regulation of tyrosine phosphorylation of Stat3 protein; muscle maintenance; response to antibiotic; monocyte chemotaxis; regulation of cell shape; positive regulation of T-helper 2 cell differentiation; positive regulation of acute inflammatory response; negative regulation of gluconeogenesis; acute-phase response; positive regulation of T cell proliferation; cell growth; response to electrical stimulus; defense response to virus; neurite development; response to drug; platelet activation; positive regulation of protein import into nucleus, translocation; positive regulation of interleukin-6 production; defense response to protozoan; negative regulation of fat cell differentiation; response to amino acid stimulus; positive regulation of chemokine production; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; response to heat; cell redox homeostasis; positive regulation of B cell activation; neutrophil apoptosis; negative regulation of protein kinase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to calcium ion; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of muscle development; positive regulation of translation; positive regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; neutrophil mediated immunity; negative regulation of caspase activity; regulation of circadian sleep/wake cycle, non-REM sleep; defense response to Gram-negative bacterium; response to insulin stimulus; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to peptidoglycan; positive regulation of cell proliferation; hepatic immune response; inflammatory response; negative regulation of chemokine biosynthetic process; aging; response to nutrient levels; cytokine and chemokine mediated signaling pathway; positive regulation of immunoglobulin secretion; endocrine pancreas development; positive regulation of peptidyl-serine phosphorylation; regulation of angiogenesis; humoral immune response; positive regulation of protein kinase B signaling cascade; positive regulation of osteoblast differentiation; response to cold; positive regulation of neuron differentiation; positive regulation of DNA replication; positive regulation of transmission of nerve impulse

Disease: Rheumatoid Arthritis, Systemic Juvenile; Arteriovenous Malformations Of The Brain; Kaposi Sarcoma, Susceptibility To; Inflammatory Bowel Disease 1
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 Research Articles on IL-6    1. Findings indicate that the IL-6 -572 allele G may be beneficial for spontaneous clearance of chronic hepatitis b virus infection.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-6 elisa kitPathways associated with IL-6 elisa kit
 Reference Product  PubMed Publications
 JUN elisa kit  >596 publications with IL-6 and JUN
 STAT3 elisa kit  >395 publications with IL-6 and STAT3
 IL1B elisa kit  >287 publications with IL-6 and IL1B
 IL17A elisa kit  >122 publications with IL-6 and IL17A
 IL6R elisa kit  >121 publications with IL-6 and IL6R
 JAK2 elisa kit  >65 publications with IL-6 and JAK2
 STAT1 elisa kit  >62 publications with IL-6 and STAT1
 SOCS3 elisa kit  >55 publications with IL-6 and SOCS3
 FOS elisa kit  >46 publications with IL-6 and FOS
 IL6ST elisa kit  >46 publications with IL-6 and IL6ST
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
Diseases associated with IL-6 elisa kitOrgans/Tissues associated with IL-6 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2417 publications with IL-6 and Inflammation
 Necrosis Antibodies  >1741 publications with IL-6 and Necrosis
 Autoimmune Diseases Antibodies  >454 publications with IL-6 and Autoimmune Diseases
 Obesity Antibodies  >301 publications with IL-6 and Obesity
 Skin Diseases Antibodies  >299 publications with IL-6 and Skin Diseases
 Arthritis, Rheumatoid Antibodies  >271 publications with IL-6 and Arthritis, Rheumatoid
 Atherosclerosis Antibodies  >244 publications with IL-6 and Atherosclerosis
 Myocardial Ischemia Antibodies  >231 publications with IL-6 and Myocardial Ischemia
 Ischemia Antibodies  >118 publications with IL-6 and Ischemia
 Diabetes Mellitus, Type 2 Antibodies  >111 publications with IL-6 and Diabetes Mellitus, Type 2
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3059 publications with IL-6 and Blood
 Vascular Antibodies  >676 publications with IL-6 and Vascular
 Bone Antibodies  >422 publications with IL-6 and Bone
 Lung Antibodies  >412 publications with IL-6 and Lung
 Brain Antibodies  >378 publications with IL-6 and Brain
 Muscle Antibodies  >305 publications with IL-6 and Muscle
 Kidney Antibodies  >272 publications with IL-6 and Kidney
 Connective Tissue Antibodies  >212 publications with IL-6 and Connective Tissue
 Skin Antibodies  >187 publications with IL-6 and Skin
 Eye Antibodies  >129 publications with IL-6 and Eye
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