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IL-8 elisa kit :: Interleukin-8 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS590014
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Wells  /  $605 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin-8 (IL-8), ELISA Kit

 Also Known As   

Interleukin-8 (IL-8)

 Product Gene Name   

IL-8 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    146930
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 Specificity    This sandwich ELISA recognizes both natural and recombinant human IL-8. This kit exhibits no detectable cross-reactivity with human IL-1?, EGF, MCAF, RANTES, and SAA.
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 Samples    Cell Culture Supernatant, serum, plasma, and other biological fluids
 Assay Type    Sandwich
 Sensitivity    The minimum detectable dose of IL-8 was determined by adding two standard deviations to the mean optical density value of the 20 zero-standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 10 pg/mL and using Calibrator Diluent II is 4 pg/mL.
 Intra-assay Precision    To determine within-run precision, three different samples of known concentration were assayed by using 20 replicates in 1 assay.
 Inter-assay Precision    To determine between-run precision, three different samples of known concentration were assayed by using replicates on 20 different assays.
 Intended Uses    This Human IL-8 ELISA kit is to be used for the in vitro quantitative determination of human interleukin-8 (IL-8) concentrations in cell culture supernatant, serum, plasma, and other biological fluids. This kit is intended for LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.
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 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-8 purchase    MBS590014 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-8 (IL-8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-8, with an estimated sensitivity of The minimum detectable dose of IL-8 was determined by adding two standard deviations to the mean optical density value of the 20 zero-standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 10 pg/mL and using Calibrator Diluent II is 4 pg/mL. The ELISA analytical biochemical technique of the MBS590014 kit is based on IL-8 antibody-IL-8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatant, serum, plasma, and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-8 elisa kit

   Principle of the assay: This IL-8 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IL-8. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IL-8, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-8 and other components of the sample. In order to quantify the amount of IL-8 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for IL-8 is added to each well to "sandwich" the IL-8 immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-8 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm +/- 2nm. In order to measure the concentration of IL-8 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus IL-8 concentration (pg/mL). The concentration of IL-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for IL-8 elisa kit    ELISA Kits for Human Cytokines and Receptors
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 Typical Testing Data/Standard Curve (for reference only) of IL-8 elisa kit    IL-8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS590014. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-8. It may not necessarily be applicable to this product.
 NCBI GI #    10834978
 NCBI GeneID    3576
 NCBI Accession #    NP_000575.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000584.3 [Other Products]
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 UniProt Secondary Accession #    Q6FGF6; Q6LAE6; Q96RG6; Q9C077; Q9UCE1; Q9UCR8; Q9UCR9; Q9UCS0; B2R4L8 [Other Products]
 UniProt Related Accession #    P10145 [Other Products]
 Molecular Weight    11,338 Da
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 NCBI Official Full Name    interleukin-8
 NCBI Official Synonym Full Names    chemokine (C-X-C motif) ligand 8
 NCBI Official Symbol    CXCL8 [Similar Products]
 NCBI Official Synonym Symbols   
IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
[Similar Products]
 NCBI Protein Information    interleukin-8; T-cell chemotactic factor; alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; emoctakin; granulocyte chemotactic protein 1; interleukin 8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; neutrophil-activating peptide 1; small inducible cytokine subfamily B, member 8; tumor necrosis factor-induced gene 1
 UniProt Protein Name    Interleukin-8
 UniProt Synonym Protein Names   
C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1
 Protein Family    Interleukin
 UniProt Gene Name    CXCL8 [Similar Products]
 UniProt Entry Name    IL8_HUMAN
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 NCBI Summary for IL-8    The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL-8    IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 4q13-q21

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; interleukin-8 receptor binding

Biological Process: regulation of cell adhesion; neutrophil chemotaxis; neutrophil activation; unfolded protein response; negative regulation of G-protein coupled receptor protein signaling pathway; calcium-mediated signaling; chemotaxis; signal transduction; regulation of retroviral genome replication; induction of positive chemotaxis; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; receptor internalization; response to molecule of bacterial origin; immune response; angiogenesis; inflammatory response; cell motility; cell cycle arrest; embryonic gut development
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 Research Articles on IL-8    1. high millimolar concentrations of ATP increased IL-8 and MCP-1 release by the glioma cells stimulated with suboptimal LPS concentration which were blocked by P2X7 and P2Y6 antagonists
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IL-8 elisa kitDiseases associated with IL-8 elisa kit
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 ATF4 Activates Genes Pathway antibodies  ATF4 Activates Genes Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >632 publications with IL-8 and Inflammation
 Necrosis Antibodies  >317 publications with IL-8 and Necrosis
 Pulmonary Disease, Chronic Obstructive Antibodies  >81 publications with IL-8 and Pulmonary Disease, Chronic Obstructive
 Adenocarcinoma Antibodies  >34 publications with IL-8 and Adenocarcinoma
 Breast Neoplasms Antibodies  >27 publications with IL-8 and Breast Neoplasms
 Kidney Diseases Antibodies  >20 publications with IL-8 and Kidney Diseases
 Colorectal Neoplasms Antibodies  >15 publications with IL-8 and Colorectal Neoplasms
 Pulmonary Fibrosis Antibodies  >15 publications with IL-8 and Pulmonary Fibrosis
 Fever Antibodies  >14 publications with IL-8 and Fever
 Prostatic Neoplasms Antibodies  >14 publications with IL-8 and Prostatic Neoplasms
Organs/Tissues associated with IL-8 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >760 publications with IL-8 and Blood
 Lung Antibodies  >247 publications with IL-8 and Lung
 Vascular Antibodies  >202 publications with IL-8 and Vascular
 Skin Antibodies  >123 publications with IL-8 and Skin
 Bone Antibodies  >72 publications with IL-8 and Bone
 Brain Antibodies  >59 publications with IL-8 and Brain
 Muscle Antibodies  >55 publications with IL-8 and Muscle
 Intestine Antibodies  >52 publications with IL-8 and Intestine
 Heart Antibodies  >51 publications with IL-8 and Heart
 Bone Marrow Antibodies  >49 publications with IL-8 and Bone Marrow
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