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M-CSF elisa kit :: Macrophage Colony Stimulating Factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS590023
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Wells  /  $605 +1 FREE 8GB USB
 
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 Product Name   

Macrophage Colony Stimulating Factor (M-CSF), ELISA Kit

 Also Known As   

Macrophage Colony Stimulating Factor (M-CSF)

 Product Gene Name   

M-CSF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    120420
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 Specificity    This sandwich ELISA can detect both natural and recombinant human M-CSF. This kit exhibits no significant cross-reactivity with human IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-7, IL-8, IL-15, IL-16, TNF-alpha, TGF-beta, and TNF-beta.
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 Samples    Serum, plasma and Cell Culture Supernatant
 Assay Type    Sandwich
 Sensitivity    The minimum detectable quantities of human M-CSF as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 15 pg/mL and 37pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities.
 Intra-assay Precision    To determine within-run precision, 3 different sera were assayed by using 10 replicates in 1 assay.
 Inter-assay Precision    To determine between-run precision, 3 different sera were assayed by using replicates on 20 different assays.
 Intended Uses    This Human M-CSF ELISA Kit is to be used for the in vitro quantitative determination of human Macrophage Colony Stimulating Factor (M-CSF) concentrations in serum, plasma and cell culture supernatant. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.
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 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of M-CSF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for M-CSF purchase    MBS590023 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Macrophage Colony Stimulating Factor (M-CSF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing M-CSF, with an estimated sensitivity of The minimum detectable quantities of human M-CSF as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 15 pg/mL and 37pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities. The ELISA analytical biochemical technique of the MBS590023 kit is based on M-CSF antibody-M-CSF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect M-CSF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, M-CSF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and Cell Culture Supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for M-CSF elisa kit

   Principle of the assay: This M-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to M-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for M-CSF and incubated. If present, M-CSF will bind and become immobilized by the antibody pre-coated on the wells and then become "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound MCSF and other components of the sample. In order to quantitatively determine the amount of M-CSF present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain M-CSF, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of M-CSF in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus M-CSF concentration (pg/mL). The concentration of M-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for M-CSF elisa kit    ELISA Kits for Human Cytokines and Receptors
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 Typical Testing Data/Standard Curve (for reference only) of M-CSF elisa kit    M-CSF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS590023. Click to request current manual
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NCBI/Uniprot data below describe general gene information for M-CSF. It may not necessarily be applicable to this product.
 NCBI GI #    27262663
 NCBI GeneID    1435
 NCBI Accession #    NP_757349.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_172210.2 [Other Products]
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 UniProt Secondary Accession #    Q13130; Q14086; Q14806; Q5VVF3; Q5VVF4; Q9UQR8; A8K6J5 [Other Products]
 UniProt Related Accession #    P09603 [Other Products]
 Molecular Weight    29,215 Da
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 NCBI Official Full Name    macrophage colony-stimulating factor 1 isoform b
 NCBI Official Synonym Full Names    colony stimulating factor 1 (macrophage)
 NCBI Official Symbol    CSF1 [Similar Products]
 NCBI Official Synonym Symbols   
MCSF; CSF-1
[Similar Products]
 NCBI Protein Information    macrophage colony-stimulating factor 1; lanimostim
 UniProt Protein Name    Macrophage colony-stimulating factor 1
 UniProt Synonym Protein Names   
Lanimostim
 UniProt Gene Name    CSF1 [Similar Products]
 UniProt Entry Name    CSF1_HUMAN
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 NCBI Summary for M-CSF    The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
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 UniProt Comments for M-CSF    M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; integral to membrane; plasma membrane; receptor complex

Molecular Function: protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of monocyte differentiation; macrophage differentiation; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; positive regulation of multicellular organism growth; osteoclast differentiation; odontogenesis; monocyte activation; positive regulation of cell proliferation; hemopoiesis; inflammatory response; positive regulation of macrophage differentiation; cell differentiation; positive regulation of cell-matrix adhesion; ossification; reproductive developmental process; positive regulation of odontogenesis of dentine-containing teeth; cell proliferation; positive regulation of mononuclear cell proliferation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; innate immune response; positive regulation of Ras protein signal transduction; regulation of ossification; positive regulation of cell migration
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 Research Articles on M-CSF    1. potent profibrotic factor in hepatitis C virus liver fibrosis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with M-CSF elisa kitPathways associated with M-CSF elisa kit
 Reference Product  PubMed Publications
 CSF1R elisa kit  >96 publications with M-CSF and CSF1R
 JUN elisa kit  >22 publications with M-CSF and JUN
 GAPDH elisa kit  >3 publications with M-CSF and GAPDH
 MYC elisa kit  >3 publications with M-CSF and MYC
 ITGB3 elisa kit  >2 publications with M-CSF and ITGB3
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Integrins In Angiogenesis Pathway antibodies  Integrins In Angiogenesis Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
Diseases associated with M-CSF elisa kitOrgans/Tissues associated with M-CSF elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >39 publications with M-CSF and Inflammation
 Carcinoma Antibodies  >26 publications with M-CSF and Carcinoma
 Necrosis Antibodies  >22 publications with M-CSF and Necrosis
 Breast Neoplasms Antibodies  >21 publications with M-CSF and Breast Neoplasms
 Lung Neoplasms Antibodies  >14 publications with M-CSF and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >10 publications with M-CSF and Neoplasms, Experimental
 Lung Diseases Antibodies  >9 publications with M-CSF and Lung Diseases
 Liver Diseases Antibodies  >7 publications with M-CSF and Liver Diseases
 Osteitis Deformans Antibodies  >6 publications with M-CSF and Osteitis Deformans
 Cardiovascular Diseases Antibodies  >4 publications with M-CSF and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >71 publications with M-CSF and Blood
 Bone Antibodies  >62 publications with M-CSF and Bone
 Lung Antibodies  >29 publications with M-CSF and Lung
 Liver Antibodies  >18 publications with M-CSF and Liver
 Brain Antibodies  >16 publications with M-CSF and Brain
 Connective Tissue Antibodies  >13 publications with M-CSF and Connective Tissue
 Lymph Node Antibodies  >12 publications with M-CSF and Lymph Node
 Intestine Antibodies  >10 publications with M-CSF and Intestine
 Kidney Antibodies  >10 publications with M-CSF and Kidney
 Spleen Antibodies  >9 publications with M-CSF and Spleen
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