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| Product Name
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Apolipoprotein L1 (APOL1), ELISA Kit
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| Also Known As
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Monkey Apolipoprotein L1 ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Manual Insert
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| OMIM
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gene 612551
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| Species Reactivity
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Monkey
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| Samples
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Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
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| Assay Type
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Competitive
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| Sensitivity
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1.0 ng/mL.
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| Intended Uses
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This ApoL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ApoL1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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| Preparation and Storage
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Store all reagents at 2-8 degree C.
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| Sample Preparation
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We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
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| Product Note
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Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
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| Other Notes
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Small volumes of APOL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for APOL1 purchase
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MBS743324 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apolipoprotein L1 (APOL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOL1, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS743324 kit is based on APOL1 antibody-APOL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for APOL1 elisa kit
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Principle of the Assay: ApoL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ApoL1 antibody and an ApoL1-HRP conjugate. The assay sample and buffer are incubated together with ApoL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoL1 concentration since ApoL1 from samples and ApoL1-HRP conjugate compete for the anti-ApoL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoL1 from the sample, fewer sites are left to bind ApoL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoL1 concentration in each sample is interpolated from this standard curve.
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| Product Categories/Family for APOL1 elisa kit
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Cardiovascular
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| Typical Testing Data/Standard Curve (for reference only) of APOL1 elisa kit
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| Sample Manual Insert of MBS743324 |
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| NCBI/Uniprot data below describe general gene information for APOL1. It may not necessarily be applicable to this product. |
| NCBI GI #
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211938440
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| NCBI GeneID
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8542
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| NCBI Accession #
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NP_001130012.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_001136540.1
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[Other Products]
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| UniProt Secondary Accession #
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O60804; Q5R3P7; Q5R3P8; Q96AB8; Q96PM4; Q9BQ03; A5PLQ4; B4DU12; E9PF24
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[Other Products]
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| UniProt Related Accession #
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O14791
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[Other Products]
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| Molecular Weight
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42,158 Da
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| NCBI Official Full Name
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apolipoprotein L1 isoform a
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| NCBI Official Synonym Full Names
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apolipoprotein L, 1
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| NCBI Official Symbol
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APOL1
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[Similar Products]
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| NCBI Official Synonym Symbols
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APOL; APO-L; FSGS4; APOL-I
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[Similar Products]
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| NCBI Protein Information
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apolipoprotein L1
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| UniProt Protein Name
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Apolipoprotein L1
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| UniProt Synonym Protein Names
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Apolipoprotein L; Apo-L; ApoL; Apolipoprotein L-I; ApoL-I
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| Protein Family
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Apolipoprotein
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| UniProt Gene Name
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APOL1
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[Similar Products]
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| UniProt Synonym Gene Names
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APOL; Apo-L; ApoL; ApoL-I
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[Similar Products]
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| UniProt Entry Name
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APOL1_HUMAN
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| NCBI Summary for APOL1
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This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
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| UniProt Comments for APOL1
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APOL1: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Lipid-binding; Channel, chloride; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: extracellular space; extracellular region; intrinsic to membrane
Molecular Function: chloride channel activity; protein binding; lipid binding
Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; killing of cells of another organism; cytolysis; innate immune response; lipoprotein metabolic process; chloride transport; lipid transport
Disease: Focal Segmental Glomerulosclerosis 4, Susceptibility To
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| Research Articles on APOL1
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1. African Americans at increased risk for APOL1-associated nephropathy (two APOL1 risk variants) with JC viruria had a lower prevalence of kidney disease, suggesting that JCV interaction with APOL1 genotype may influence kidney disease risk.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with APOL1 elisa kit | Pathways associated with APOL1 elisa kit |
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| Diseases associated with APOL1 elisa kit | Organs/Tissues associated with APOL1 elisa kit |
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ELISA Kit 
