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Arg1 elisa kit :: Monkey Arginase 1 (Liver-type) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS748033
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Arginase 1 (Liver-type) (Arg1), ELISA Kit

★Popular Item★
 Also Known As   

Monkey Arginase 1 (Liver-type) ELISA Kit

 Product Gene Name   

Arg1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    608313
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 Species Reactivity    Monkey
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 Specificity    This assay has high sensitivity and excellent specificity for detection of L-ARG1. No significant cross-reactivity or interference between L-ARG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between L-ARG1 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    5-100ng/mL
 Sensitivity    1.0ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Arg1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Arg1 purchase    MBS748033 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Arginase 1 (Liver-type) (Arg1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5-100ng/mL in biological research samples containing Arg1, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS748033 kit is based on Arg1 antibody-Arg1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Arg1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Arg1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for Arg1 elisa kit

   Intended Uses: This L-ARG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey L-ARG1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: L-ARG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-L-ARG1 antibody and an L-ARG1-HRP conjugate. The assay sample and buffer are incubated together with L-ARG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the L-ARG1 concentration since L-ARG1 from samples and L-ARG1-HRP conjugate compete for the anti-L-ARG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by L-ARG1 from the sample, fewer sites are left to bind L-ARG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The L-ARG1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Arg1 elisa kit    Signal Transduction
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 Typical Standard Curve/Testing Data of Arg1 elisa kit    Arg1 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS748033. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Arg1. It may not necessarily be applicable to this product.
 NCBI GI #    346986435
 NCBI GeneID    383
 NCBI Accession #    NP_001231367.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001244438.1 [Other Products]
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 UniProt Secondary Accession #    Q5JWT5; Q5JWT6; Q8TE72; Q9BS50; A6NEA0 [Other Products]
 UniProt Related Accession #    P05089 [Other Products]
 Molecular Weight    25,356 Da
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 NCBI Official Full Name    arginase-1 isoform 1
 NCBI Official Synonym Full Names    arginase 1
 NCBI Official Symbol    ARG1 [Similar Products]
 NCBI Protein Information    arginase-1; arginase, liver; type I arginase; liver-type arginase
 UniProt Protein Name    Arginase-1
 UniProt Synonym Protein Names   
Liver-type arginase; Type I arginase
 Protein Family    Arginase
 UniProt Gene Name    ARG1 [Similar Products]
 UniProt Entry Name    ARGI1_HUMAN
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 NCBI Summary for Arg1    Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
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 UniProt Comments for Arg1    ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.3.1; Hydrolase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: extracellular space; neuron projection; cell soma; cytoplasm; cytosol; nucleus

Molecular Function: manganese ion binding; arginase activity

Biological Process: response to drug; mammary gland involution; maternal process involved in pregnancy; response to herbicide; response to amino acid stimulus; liver development; response to vitamin A; response to manganese ion; response to selenium ion; response to vitamin E; response to cadmium ion; response to zinc ion; response to methylmercury; arginine catabolic process; positive regulation of endothelial cell proliferation; response to axon injury; collagen biosynthetic process; lung development; response to amine stimulus; urea cycle

Disease: Argininemia
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 Research Articles on Arg1    1. Serum arginase I might regulate serum L-arginine and 3-nitrotyrosine via L-arginine.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Arg1 elisa kitPathways associated with Arg1 elisa kit
 Reference Product  PubMed Publications
 NOS2 elisa kit  >53 publications with Arg1 and NOS2
 NOS3 elisa kit  >11 publications with Arg1 and NOS3
 STAT3 elisa kit  >6 publications with Arg1 and STAT3
 ASL elisa kit  >6 publications with Arg1 and ASL
 NOS1 elisa kit  >6 publications with Arg1 and NOS1
 OTC elisa kit  >5 publications with Arg1 and OTC
 OAT elisa kit  >2 publications with Arg1 and OAT
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 IL4-mediated Signaling Events Pathway antibodies  IL4-mediated Signaling Events Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
Diseases associated with Arg1 elisa kitOrgans/Tissues associated with Arg1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >100 publications with Arg1 and Inflammation
 Lung Diseases Antibodies  >58 publications with Arg1 and Lung Diseases
 Disease Models, Animal Antibodies  >57 publications with Arg1 and Disease Models, Animal
 Nervous System Diseases Antibodies  >47 publications with Arg1 and Nervous System Diseases
 Immune System Diseases Antibodies  >40 publications with Arg1 and Immune System Diseases
 Necrosis Antibodies  >32 publications with Arg1 and Necrosis
 Cardiovascular Diseases Antibodies  >29 publications with Arg1 and Cardiovascular Diseases
 Asthma Antibodies  >27 publications with Arg1 and Asthma
 Fibrosis Antibodies  >22 publications with Arg1 and Fibrosis
 Lung Neoplasms Antibodies  >21 publications with Arg1 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >193 publications with Arg1 and Blood
 Muscle Antibodies  >108 publications with Arg1 and Muscle
 Lung Antibodies  >84 publications with Arg1 and Lung
 Brain Antibodies  >82 publications with Arg1 and Brain
 Liver Antibodies  >57 publications with Arg1 and Liver
 Bone Marrow Antibodies  >34 publications with Arg1 and Bone Marrow
 Eye Antibodies  >26 publications with Arg1 and Eye
 Spleen Antibodies  >25 publications with Arg1 and Spleen
 Skin Antibodies  >16 publications with Arg1 and Skin
 Uterus Antibodies  >10 publications with Arg1 and Uterus
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