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COL11A2 elisa kit :: Monkey Collagen alpha-2 (XI) chain (COL11A2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7226382
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
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 Product Name   

Collagen alpha-2 (XI) chain (COL11A2), ELISA Kit

★Popular Item★
 Also Known As   

Monkey Collagen alpha-2 (XI) chain (COL11A2) ELISA Kit

 Product Gene Name   

COL11A2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    259420
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 Species Reactivity    Monkey
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 Specificity    This assay has high sensitivity and excellent specificity for detection of COL11alpha2. No significant cross-reactivity or interference between COL11alpha2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COL11alpha2 and all the analogues, therefore, cross reaction may still exist in some cases
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of COL11A2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for COL11A2 purchase    MBS7226382 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen alpha-2 (XI) chain (COL11A2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing COL11A2, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7226382 kit is based on COL11A2 antibody-COL11A2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL11A2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL11A2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for COL11A2 elisa kit

   Intended Uses: This COL11alpha2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey COL11alpha2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: COL11alpha2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COL11alpha2 antibody and an COL11 alpha2-HRP conjugate. The assay sample and buffer are incubated together with COL11alpha2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL11alpha2 concentration since COL11alpha2 from samples and COL11 alpha2-HRP conjugate compete for the anti-COL11alpha2 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL11 alpha2 from the sample, fewer sites are left to bind COL11alpha2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL11alpha2 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for COL11A2 elisa kit    Signal Transduction
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 Typical Standard Curve/Testing Data of COL11A2 elisa kit    COL11A2 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS7226382. Click to request current manual
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NCBI/Uniprot data below describe general gene information for COL11A2. It may not necessarily be applicable to this product.
 NCBI GI #    296439507
 NCBI GeneID    1278
 NCBI Accession #    P08123.7 [Other Products]
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 UniProt Secondary Accession #    P02464; Q13897; Q13997; Q13998; Q14038; Q14057; Q15177; Q15947; Q16480; Q16511; Q7Z5S6 [Other Products]
 UniProt Related Accession #    P08123 [Other Products]
 Molecular Weight    129,314 Da
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 NCBI Official Full Name    Collagen alpha-2(I) chain
 NCBI Official Synonym Full Names    collagen, type I, alpha 2
 NCBI Official Symbol    COL1A2 [Similar Products]
 NCBI Official Synonym Symbols   
OI4
[Similar Products]
 NCBI Protein Information    collagen alpha-2(I) chain; collagen alpha-2(I) chain; type I procollagen; alpha 2(I)-collagen; alpha-2 type I collagen; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-2 chain
 UniProt Protein Name    Collagen alpha-2(I) chain
 UniProt Synonym Protein Names   
Alpha-2 type I collagen
 Protein Family    Collagen
 UniProt Gene Name    COL1A2 [Similar Products]
 UniProt Entry Name    CO1A2_HUMAN
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 NCBI Summary for COL11A2    This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
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 UniProt Comments for COL11A2    COL1A2: Type I collagen is a member of group I collagen (fibrillar forming collagen). Defects in COL1A2 are the cause of Ehlers-Danlos syndrome type 7B (EDS7B). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7B is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations. Defects in COL1A2 are a cause of osteogenesis imperfecta type 1 (OI1). A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta. Defects in COL1A2 are a cause of osteogenesis imperfecta type 2 (OI2); also known as osteogenesis imperfecta congenita (OIC) or lethal perinatal. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency. Defects in COL1A2 are the cause of Ehlers-Danlos syndrome autosomal recessive cardiac valvular form (EDSCV). A connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. In addition to joint laxity, skin hyperextensibility and friability, and abnormal scar formation, patients have mitral valve prolapse and insufficiency, mitral regurgitation, and aortic insufficiency. Defects in COL1A2 are a cause of osteogenesis imperfecta type 3 (OI3). A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta. Defects in COL1A2 are a cause of osteogenesis imperfecta type 4 (OI4); also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta. A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1. Belongs to the fibrillar collagen family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; extracellular region; collagen type I

Molecular Function: protein binding, bridging; identical protein binding; protein binding; metal ion binding; extracellular matrix structural constituent; platelet-derived growth factor binding; SMAD binding

Biological Process: platelet activation; blood vessel development; receptor-mediated endocytosis; extracellular matrix organization and biogenesis; collagen fibril organization; skin morphogenesis; Rho protein signal transduction; odontogenesis; extracellular matrix disassembly; collagen catabolic process; regulation of blood pressure; transforming growth factor beta receptor signaling pathway; blood coagulation; skeletal development; leukocyte migration

Disease: Ehlers-danlos Syndrome, Type Vii, Autosomal Dominant; Osteogenesis Imperfecta, Type Ii; Ehlers-danlos Syndrome, Autosomal Recessive, Cardiac Valvular Form; Osteogenesis Imperfecta, Type Iii; Osteoporosis; Osteogenesis Imperfecta, Type Iv
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 Research Articles on COL11A2    1. this study highlights an essential role for Sin3B in IFN-c induced COL1A2 repression in smooth muscle cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with COL11A2 elisa kitDiseases associated with COL11A2 elisa kit
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Collagen Biosynthesis And Modifying Enzymes Pathway antibodies  Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Endothelins Pathway antibodies  Endothelins Pathway Diagram
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >184 publications with COL11A2 and Fibrosis
 Cardiovascular Diseases Antibodies  >92 publications with COL11A2 and Cardiovascular Diseases
 Liver Diseases Antibodies  >42 publications with COL11A2 and Liver Diseases
 Inflammation Antibodies  >39 publications with COL11A2 and Inflammation
 Osteoporosis Antibodies  >36 publications with COL11A2 and Osteoporosis
 Heart Diseases Antibodies  >34 publications with COL11A2 and Heart Diseases
 Necrosis Antibodies  >32 publications with COL11A2 and Necrosis
 Liver Cirrhosis Antibodies  >30 publications with COL11A2 and Liver Cirrhosis
 Kidney Diseases Antibodies  >23 publications with COL11A2 and Kidney Diseases
 Carcinoma Antibodies  >22 publications with COL11A2 and Carcinoma
Organs/Tissues associated with COL11A2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >270 publications with COL11A2 and Bone
 Connective Tissue Antibodies  >195 publications with COL11A2 and Connective Tissue
 Skin Antibodies  >188 publications with COL11A2 and Skin
 Muscle Antibodies  >77 publications with COL11A2 and Muscle
 Blood Antibodies  >73 publications with COL11A2 and Blood
 Liver Antibodies  >70 publications with COL11A2 and Liver
 Embryonic Tissue Antibodies  >50 publications with COL11A2 and Embryonic Tissue
 Vascular Antibodies  >48 publications with COL11A2 and Vascular
 Lung Antibodies  >42 publications with COL11A2 and Lung
 Heart Antibodies  >42 publications with COL11A2 and Heart
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