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Cy.C elisa kit :: Monkey Cytochrome C ELISA Kit

Scan QR to view Datasheet Catalog #    MBS743215
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cytochrome C (Cy.C), ELISA Kit

★Popular Item★
 Also Known As   

Monkey Cytochrome C ELISA Kit

 Product Gene Name   

Cy.C elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 612004
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 Species Reactivity    Monkey
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    2.5-50ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This Cy.C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey Cy.C. This ELISA kit for research use only, not for therapeutic or diagnostic applications
 !Intended Uses    This Cy.C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey Cy.C. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Cy.C elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Cy.C purchase    MBS743215 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome C (Cy.C) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50ng/mL in biological research samples containing Cy.C, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS743215 kit is based on Cy.C antibody-Cy.C antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cy.C antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Cy.C. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Cy.C elisa kit

   Principle of the Assay: Cy.C ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti- Cy.C antibody and an Cy.C-HRP conjugate.The assay sample and buffer are incubated together with Cy.C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Cy.C concentration since Cy.C from samples and Cy.C-HRP conjugate compete for the anti-Cy.C antibody binding site. Since the number of sites is limited, as more sites are occupied by Cy.C from the sample, fewer sites are left to bind Cy.C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Cy.C concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Cy.C elisa kit    Cancer
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 Typical Testing Data/Standard Curve (for reference only) of Cy.C elisa kit    Cy.C elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS743215. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Cy.C. It may not necessarily be applicable to this product.
 NCBI GI #    11128019
 NCBI GeneID    54205
 NCBI Accession #    NP_061820.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_018947.5 [Other Products]
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 UniProt Secondary Accession #    P00001; Q6NUR2; Q6NX69; Q96BV4; A4D166; B2R4I1 [Other Products]
 UniProt Related Accession #    P99999 [Other Products]
 Molecular Weight    11,749 Da
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 NCBI Official Full Name    cytochrome c
 NCBI Official Synonym Full Names    cytochrome c, somatic
 NCBI Official Symbol    CYCS [Similar Products]
 NCBI Official Synonym Symbols   
CYC; HCS; THC4
[Similar Products]
 NCBI Protein Information    cytochrome c
 UniProt Protein Name    Cytochrome c
 Protein Family    Cytochrome
 UniProt Gene Name    CYCS [Similar Products]
 UniProt Synonym Gene Names    CYC [Similar Products]
 UniProt Entry Name    CYC_HUMAN
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 NCBI Summary for Cy.C    This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
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 UniProt Comments for Cy.C    CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family.

Protein type: Oxidoreductase; Protein phosphatase, Ser/Thr (non-receptor); Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: protein phosphatase type 2A complex; mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; nucleus; cytosol

Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; protein binding; iron ion binding; heme binding; protein serine/threonine phosphatase activity

Biological Process: mitochondrion organization and biogenesis; cellular metabolic process; response to reactive oxygen species; dephosphorylation; apoptosis; organelle organization and biogenesis; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; DNA fragmentation during apoptosis; caspase activation via cytochrome c; transmembrane transport; mitochondrial electron transport, ubiquinol to cytochrome c

Disease: Thrombocytopenia 4
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 Research Articles on Cy.C    1. proposed that mutation of residue 41, and interaction with cardiolipin, increase peroxidase activity by altering the 40-57 Omega loop and its hydrogen bond network with the propionate of haem ring A; these changes enhance access of hydrogen peroxide and substrate to the haem
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Cy.C elisa kitPathways associated with Cy.C elisa kit
 Reference Product  PubMed Publications
 BCL2 elisa kit  >3 publications with Cy.C and BCL2
 APAF1 elisa kit  >2 publications with Cy.C and APAF1
 PPP2CA elisa kit  >1 publications with Cy.C and PPP2CA
 CYC1 elisa kit  >1 publications with Cy.C and CYC1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway antibodies  Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
Diseases associated with Cy.C elisa kitOrgans/Tissues associated with Cy.C elisa kit
 Disease Name  Pubmed Publications
 Thrombocytopenia Antibodies  >6 publications with Cy.C and Thrombocytopenia
 Nervous System Diseases Antibodies  >1 publications with Cy.C and Nervous System Diseases
 Lung Diseases Antibodies  >1 publications with Cy.C and Lung Diseases
 Neoplasm Metastasis Antibodies  >1 publications with Cy.C and Neoplasm Metastasis
 Hypertrophy Antibodies  >1 publications with Cy.C and Hypertrophy
 Neoplasms, Experimental Antibodies  >1 publications with Cy.C and Neoplasms, Experimental
 Myocardial Infarction Antibodies  >1 publications with Cy.C and Myocardial Infarction
 Fibrosis Antibodies  >1 publications with Cy.C and Fibrosis
 Thrombocytopenia 4 Antibodies  >1 publications with Cy.C and Thrombocytopenia 4
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4 publications with Cy.C and Blood
 Prostate Antibodies  >3 publications with Cy.C and Prostate
 Liver Antibodies  >2 publications with Cy.C and Liver
 Lung Antibodies  >2 publications with Cy.C and Lung
 Vascular Antibodies  >2 publications with Cy.C and Vascular
 Bone Antibodies  >2 publications with Cy.C and Bone
 Intestine Antibodies  >1 publications with Cy.C and Intestine
 Muscle Antibodies  >1 publications with Cy.C and Muscle
 Skin Antibodies  >1 publications with Cy.C and Skin
 Testis Antibodies  >1 publications with Cy.C and Testis
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