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EPO elisa kit :: Monkey EPO ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2509449
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $400 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,975 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,480 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EPO, ELISA Kit

★Popular Item★
 Also Known As   

Monkey EPO (Erythropoietin) ELISA Kit

 Product Gene Name   

EPO elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2509449 COA    COA PDF
 OMIM    133170
 3D Structure    ModBase 3D Structure for P01588
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 Species Reactivity    Monkey
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 Specificity    This kit recognizes natural and some recombinant Monkey EPO. No significant crossreactivity or interference between Monkey EPO and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    31.25-2000pg/mL
 Sensitivity    Min: 18.75pg/mL; Max: 2000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EPO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EPO purchase    MBS2509449 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EPO, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing EPO, with an estimated sensitivity of Min: 18.75pg/mL; Max: 2000pg/mL. The ELISA analytical biochemical technique of the MBS2509449 kit is based on EPO antibody-EPO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EPO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EPO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EPO elisa kit

   Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Monkey EPO concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Monkey EPO. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Monkey EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Monkey EPO, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey EPO. The concentration of Monkey EPO in samples can be calculated by comparing the OD of the samples with the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of EPO elisa kit    EPO elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2509449. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EPO. It may not necessarily be applicable to this product.
 NCBI GI #    62240997
 NCBI GeneID    2056
 NCBI Accession #    NP_000790.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000799.2 [Other Products]
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 UniProt Primary Accession #    P01588 [Other Products]
 UniProt Secondary Accession #    Q2M2L6; Q549U2; Q9UDZ0; Q9UEZ5; Q9UHA0 [Other Products]
 UniProt Related Accession #    P01588 [Other Products]
 Molecular Weight    21,307 Da
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 NCBI Official Full Name    erythropoietin
 NCBI Official Synonym Full Names    erythropoietin
 NCBI Official Symbol    EPO [Similar Products]
 NCBI Official Synonym Symbols   
EP; MVCD2
[Similar Products]
 NCBI Protein Information    erythropoietin; epoetin
 UniProt Protein Name    Erythropoietin
 UniProt Synonym Protein Names   
INN: Epoetin
 Protein Family    Erythropoietin
 UniProt Gene Name    EPO [Similar Products]
 UniProt Entry Name    EPO_HUMAN
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 NCBI Summary for EPO    This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]
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 UniProt Comments for EPO    EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: protein binding; erythropoietin receptor binding; hormone activity; protein kinase activator activity

Biological Process: hemoglobin biosynthetic process; erythrocyte maturation; apoptosis; blood circulation; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; response to testosterone stimulus; signal transduction; response to salt stress; response to vitamin A; peptidyl-serine phosphorylation; response to hyperoxia; positive regulation of tyrosine phosphorylation of Stat5 protein; response to estrogen stimulus; positive regulation of protein kinase activity; positive regulation of cell proliferation; response to axon injury; positive regulation of Ras protein signal transduction; response to electrical stimulus; positive regulation of neuron differentiation; positive regulation of DNA replication; embryo implantation; aging

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2
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 Research Articles on EPO    1. The main aim of this study was to investigate the dose-response relationship and determine the lowest effective dose of Erythropoietin(EPO) that reliably increases the osteogenic differentiation of human mesenchymal stromal cells (hMSCs).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with EPO elisa kitPathways associated with EPO elisa kit
 Reference Product  PubMed Publications
 EPOR elisa kit  >723 publications with EPO and EPOR
 JAK2 elisa kit  >325 publications with EPO and JAK2
 TNF elisa kit  >285 publications with EPO and TNF
 HIF1A elisa kit  >259 publications with EPO and HIF1A
 STAT3 elisa kit  >81 publications with EPO and STAT3
 VEGFA elisa kit  >46 publications with EPO and VEGFA
 GATA2 elisa kit  >28 publications with EPO and GATA2
 TFRC elisa kit  >4 publications with EPO and TFRC
 Products by Pathway  Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 EPO Receptor Signaling Pathway antibodies  EPO Receptor Signaling Pathway Diagram
 EPO Signaling Pathway antibodies  EPO Signaling Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 HIF-2-alpha Transcription Factor Network Pathway antibodies  HIF-2-alpha Transcription Factor Network Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
Diseases associated with EPO elisa kitOrgans/Tissues associated with EPO elisa kit
 Disease Name  Pubmed Publications
 Anemia Antibodies  >2800 publications with EPO and Anemia
 Kidney Diseases Antibodies  >1368 publications with EPO and Kidney Diseases
 Renal Insufficiency Antibodies  >1005 publications with EPO and Renal Insufficiency
 Kidney Failure, Chronic Antibodies  >925 publications with EPO and Kidney Failure, Chronic
 Vascular Diseases Antibodies  >881 publications with EPO and Vascular Diseases
 Nervous System Diseases Antibodies  >687 publications with EPO and Nervous System Diseases
 Inflammation Antibodies  >591 publications with EPO and Inflammation
 Central Nervous System Diseases Antibodies  >516 publications with EPO and Central Nervous System Diseases
 Necrosis Antibodies  >368 publications with EPO and Necrosis
 Heart Diseases Antibodies  >364 publications with EPO and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >805 publications with EPO and Brain
 Eye Antibodies  >158 publications with EPO and Eye
 Testis Antibodies  >33 publications with EPO and Testis
 Prostate Antibodies  >32 publications with EPO and Prostate
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