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GLP-2 elisa kit :: Monkey Glucagon Like Peptide 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS009996 GLP-2 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glucagon Like Peptide 2 (GLP-2), ELISA Kit

 Also Known As   

Monkey Glucagon Like Peptide 2 ELISA Kit

 Product Gene Name   

GLP-2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    138030
 3D Structure    ModBase 3D Structure for P01275
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 Species Reactivity    Monkey
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 Specificity    No significant cross-reactivity or interference between Monkey GLP-2 and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    0.25 ng/ml - 8 ng/ml.
 Sensitivity    0.1 ng/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GLP-2 concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey GLP-2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GLP-2 and GLP-2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GLP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GLP-2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GLP-2 purchase    MBS009996 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glucagon Like Peptide 2 (GLP-2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.25 ng/ml - 8 ng/ml in biological research samples containing GLP-2, with an estimated sensitivity of 0.1 ng/ml. The ELISA analytical biochemical technique of the MBS009996 kit is based on GLP-2 antibody-GLP-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GLP-2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GLP-2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for GLP-2 elisa kit

   Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GLP-2 concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey GLP-2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GLP-2 and GLP-2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GLP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS009996. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GLP-2. It may not necessarily be applicable to this product.
 NCBI GI #    4503945
 NCBI GeneID    2641
 NCBI Accession #    NP_002045.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002054.4 [Other Products]
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 UniProt Primary Accession #    P01275 [Other Products]
 UniProt Secondary Accession #    Q53TP6; A6NN65 [Other Products]
 UniProt Related Accession #    P01275 [Other Products]
 Molecular Weight    20,909 Da
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 NCBI Official Full Name    glucagon preproprotein
 NCBI Official Synonym Full Names    glucagon
 NCBI Official Symbol    GCG [Similar Products]
 NCBI Official Synonym Symbols   
GLP1; GLP2; GRPP
[Similar Products]
 NCBI Protein Information    glucagon; preproglucagon; glucagon-like peptide 1; glucagon-like peptide 2; glicentin-related polypeptide
 UniProt Protein Name    Glucagon
 UniProt Synonym Protein Names   
Incretin hormone
 UniProt Gene Name    GCG [Similar Products]
 UniProt Synonym Gene Names    GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2 [Similar Products]
 UniProt Entry Name    GLUC_HUMAN
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 NCBI Summary for GLP-2    The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GLP-2    GCG: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. GLP-1 and GLP-2 are induced in response to nutrient ingestion. Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. Belongs to the glucagon family.

Protein type: Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 2q36-q37

Cellular Component: extracellular space; endoplasmic reticulum lumen; extracellular region; plasma membrane

Molecular Function: identical protein binding; protein binding; hormone activity; glucagon receptor binding; receptor binding

Biological Process: positive regulation of protein binding; positive regulation of histone H3-K4 methylation; signal transduction; positive regulation of peptidyl-serine phosphorylation; response to starvation; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; cell proliferation; cellular protein metabolic process; positive regulation of protein kinase activity; positive regulation of cAMP biosynthetic process; energy reserve metabolic process; feeding behavior; regulation of insulin secretion; negative regulation of apoptosis
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 Research Articles on GLP-2    1. findings show genetic polymorphism in rs12104705 of GCG may interact with smoking and physical activity to modify the risk of type 2 diabetes mellitus in Han Chinese
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GLP-2 elisa kitPathways associated with GLP-2 elisa kit
 Reference Product  PubMed Publications
 INS elisa kit  >12 publications with GLP-2 and INS
 GLP1R elisa kit  >7 publications with GLP-2 and GLP1R
 GCGR elisa kit  >5 publications with GLP-2 and GCGR
 SST elisa kit  >4 publications with GLP-2 and SST
 GIPR elisa kit  >2 publications with GLP-2 and GIPR
 SCT elisa kit  >2 publications with GLP-2 and SCT
 FOXA2 elisa kit  >2 publications with GLP-2 and FOXA2
 Products by Pathway  Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Glucagon Signaling In Metabolic Regulation Pathway antibodies  Glucagon Signaling In Metabolic Regulation Pathway Diagram
 Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway antibodies  Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway Diagram
 Glucagon-type Ligand Receptors Pathway antibodies  Glucagon-type Ligand Receptors Pathway Diagram
Diseases associated with GLP-2 elisa kitOrgans/Tissues associated with GLP-2 elisa kit
 Disease Name  Pubmed Publications
 Muscular Diseases Antibodies  >72 publications with GLP-2 and Muscular Diseases
 Diabetes Mellitus, Type 2 Antibodies  >23 publications with GLP-2 and Diabetes Mellitus, Type 2
 Vascular Diseases Antibodies  >15 publications with GLP-2 and Vascular Diseases
 Obesity Antibodies  >14 publications with GLP-2 and Obesity
 Insulin Resistance Antibodies  >12 publications with GLP-2 and Insulin Resistance
 Kidney Diseases Antibodies  >11 publications with GLP-2 and Kidney Diseases
 Hyperglycemia Antibodies  >10 publications with GLP-2 and Hyperglycemia
 Hyperinsulinism Antibodies  >9 publications with GLP-2 and Hyperinsulinism
 Heart Diseases Antibodies  >8 publications with GLP-2 and Heart Diseases
 Liver Diseases Antibodies  >7 publications with GLP-2 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >34 publications with GLP-2 and Pancreas
 Intestine Antibodies  >17 publications with GLP-2 and Intestine
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