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Grb7 elisa kit :: Monkey Grb7 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2504909 Grb7 elisa kit
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Grb7, ELISA Kit

 Also Known As   

Monkey Grb7 (Growth Factor Receptor Bound Protein 7) ELISA Kit

 Product Gene Name   

Grb7 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601522
 3D Structure    ModBase 3D Structure for Q14451
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 Species Reactivity    Monkey
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 Specificity    This kit recognizes natural and recombinant Monkey Grb7. No significant cross-reactivity or interference between Monkey Grb7 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    62.5-4000pg/mL
 Sensitivity    37.5pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Grb7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Grb7 purchase    MBS2504909 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Grb7, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4000pg/mL in biological research samples containing Grb7, with an estimated sensitivity of 37.5pg/mL. The ELISA analytical biochemical technique of the MBS2504909 kit is based on Grb7 antibody-Grb7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Grb7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Grb7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Grb7 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey Grb7 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Grb7. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Grb7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Grb7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Grb7. You can calculate the concentration of Grb7 in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2504909. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Grb7. It may not necessarily be applicable to this product.
 NCBI GI #    334883159
 NCBI GeneID    2886
 NCBI Accession #    NP_001229371.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001242442.1 [Other Products]
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 UniProt Primary Accession #    Q14451 [Other Products]
 UniProt Secondary Accession #    Q53YD3; Q92568; Q96DF9; Q9Y220; B2RAV1; B3KNL0; B3KWP9; B7WP75; J3KQM4 [Other Products]
 UniProt Related Accession #    Q14451 [Other Products]
 Molecular Weight    55,920 Da
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 NCBI Official Full Name    growth factor receptor-bound protein 7 isoform b
 NCBI Official Synonym Full Names    growth factor receptor-bound protein 7
 NCBI Official Symbol    GRB7 [Similar Products]
 NCBI Protein Information    growth factor receptor-bound protein 7; B47; GRB7 adapter protein; epidermal growth factor receptor GRB-7
 UniProt Protein Name    Growth factor receptor-bound protein 7
 UniProt Synonym Protein Names   
B47; Epidermal growth factor receptor GRB-7; GRB7 adapter protein
 Protein Family    Growth factor receptor-bound protein
 UniProt Gene Name    GRB7 [Similar Products]
 UniProt Entry Name    GRB7_HUMAN
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 NCBI Summary for Grb7    The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
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 UniProt Comments for Grb7    GRB7: Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress. Homodimer. Interacts (via SH2 domain) with EGFR, ERBB2, ERBB3 (when phosphorylated), ERBB4 (when phosphorylated), EPHB1, INSR, FGFR1, PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with SHC1. Interacts with RND1. Interacts (when tyrosine phosphorylated) with FHL2 and HAX1. Interacts (via SH2 domain) with RET and PTK2/FAK1. Interacts (when not phosphorylated) with ELAVL1. In stressed cells, but not in normal cells, part of a complex that contains at least GRB7, PTK2/FAK1, STAU1, ELAVL1 and TIA1. Interacts (via SH2 domain) with KIT (phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Belongs to the GRB7/10/14 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cell projection; focal adhesion; stress granule; plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; RNA binding; protein kinase binding; phosphoinositide binding

Biological Process: epidermal growth factor receptor signaling pathway; stress granule assembly; positive regulation of signal transduction; negative regulation of translation; blood coagulation; leukocyte migration; positive regulation of cell migration
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 Research Articles on Grb7    1. apo Grb7 SH2 domain crystallized in the trigonal space group P63, whereas the G7-B1-Grb7 SH2 domain complex crystallized in the monoclinic space group P21
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Grb7 elisa kitPathways associated with Grb7 elisa kit
 Reference Product  PubMed Publications
 SRC elisa kit  >86 publications with Grb7 and SRC
 ERBB2 elisa kit  >81 publications with Grb7 and ERBB2
 KIT elisa kit  >8 publications with Grb7 and KIT
 ERBB3 elisa kit  >7 publications with Grb7 and ERBB3
 RET elisa kit  >5 publications with Grb7 and RET
 TEK elisa kit  >3 publications with Grb7 and TEK
 EPHB1 elisa kit  >3 publications with Grb7 and EPHB1
 FYN elisa kit  >2 publications with Grb7 and FYN
 RND1 elisa kit  >1 publications with Grb7 and RND1
 Products by Pathway  Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 EPHB Forward Signaling Pathway antibodies  EPHB Forward Signaling Pathway Diagram
 GRB7 Events In ERBB2 Signaling Pathway antibodies  GRB7 Events In ERBB2 Signaling Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Kit Receptor Signaling Pathway antibodies  Kit Receptor Signaling Pathway Diagram
 PDGFR-beta Signaling Pathway antibodies  PDGFR-beta Signaling Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
Diseases associated with Grb7 elisa kitOrgans/Tissues associated with Grb7 elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >55 publications with Grb7 and Breast Neoplasms
 Adenocarcinoma Antibodies  >14 publications with Grb7 and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >11 publications with Grb7 and Neoplasms, Experimental
 Disease Models, Animal Antibodies  >6 publications with Grb7 and Disease Models, Animal
 Inflammation Antibodies  >4 publications with Grb7 and Inflammation
 Necrosis Antibodies  >3 publications with Grb7 and Necrosis
 Ovarian Neoplasms Antibodies  >3 publications with Grb7 and Ovarian Neoplasms
 Pancreatic Neoplasms Antibodies  >2 publications with Grb7 and Pancreatic Neoplasms
 Anemia Antibodies  >1 publications with Grb7 and Anemia
 Carcinoma, Hepatocellular Antibodies  >1 publications with Grb7 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >11 publications with Grb7 and Stomach
 Lymph Node Antibodies  >9 publications with Grb7 and Lymph Node
 Liver Antibodies  >7 publications with Grb7 and Liver
 Kidney Antibodies  >5 publications with Grb7 and Kidney
 Testis Antibodies  >4 publications with Grb7 and Testis
 Mammary Gland Antibodies  >3 publications with Grb7 and Mammary Gland
 Prostate Antibodies  >3 publications with Grb7 and Prostate
 Eye Antibodies  >3 publications with Grb7 and Eye
 Esophagus Antibodies  >3 publications with Grb7 and Esophagus
 Embryonic Tissue Antibodies  >3 publications with Grb7 and Embryonic Tissue
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