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IL18 elisa kit :: Monkey Interleukin 18 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS744025
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 18 (IL18), ELISA Kit

★Popular Item★
 Also Known As   

Monkey Interleukin 18 ELISA Kit

 Product Gene Name   

IL18 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Monkey
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IL-18. No significant cross-reactivity or interference between IL-18 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-18 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL18 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL18 purchase    MBS744025 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 18 (IL18) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing IL18, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS744025 kit is based on IL18 antibody-IL18 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL18 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL18. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL18 elisa kit

   Intended Uses: This IL-18 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IL-18. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: IL-18 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-18 antibody and an IL-18-HRP conjugate. The assay sample and buffer are incubated together with IL-18-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-18 concentration since IL-18 from samples and IL-18-HRP conjugate compete for the anti-IL-18 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-18 from the sample, fewer sites are left to bind IL-18-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-18 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for IL18 elisa kit    Immunology
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 Typical Standard Curve/Testing Data of IL18 elisa kit    IL18 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS744025. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL18. It may not necessarily be applicable to this product.
 NCBI GI #    9506805
 NCBI GeneID    29197
 NCBI Accession #    NP_062038.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_019165.1 [Other Products]
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 UniProt Secondary Accession #    O88749; P97637; Q541E6 [Other Products]
 UniProt Related Accession #    P97636 [Other Products]
 Molecular Weight    20,056 Da
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 NCBI Official Full Name    interleukin-18
 NCBI Official Synonym Full Names    interleukin 18
 NCBI Official Symbol    Il18 [Similar Products]
 NCBI Official Synonym Symbols   
IL-18
[Similar Products]
 NCBI Protein Information    interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor
 UniProt Protein Name    Interleukin-18
 UniProt Synonym Protein Names   
Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma
 Protein Family    Interleukin
 UniProt Gene Name    Il18 [Similar Products]
 UniProt Synonym Gene Names    Igif; IL-18; IFN-gamma-inducing factor; IL-1 gamma [Similar Products]
 UniProt Entry Name    IL18_RAT
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 NCBI Summary for IL18    a pro-inflammatory cytokine; acts as a modulator of immune functions [RGD, Feb 2006]
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 UniProt Comments for IL18    Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
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 Research Articles on IL18    1. IL-18 serves as a positive regulator of hepatocyte proliferation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IL18 elisa kitDiseases associated with IL18 elisa kit
 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Hypertrophy Model Pathway antibodies  Hypertrophy Model Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1182 publications with IL18 and Inflammation
 Necrosis Antibodies  >789 publications with IL18 and Necrosis
 Cardiovascular Diseases Antibodies  >425 publications with IL18 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >405 publications with IL18 and Disease Models, Animal
 Lung Diseases Antibodies  >325 publications with IL18 and Lung Diseases
 Kidney Diseases Antibodies  >301 publications with IL18 and Kidney Diseases
 Hypersensitivity Antibodies  >265 publications with IL18 and Hypersensitivity
 Liver Diseases Antibodies  >235 publications with IL18 and Liver Diseases
 Arthritis, Rheumatoid Antibodies  >220 publications with IL18 and Arthritis, Rheumatoid
 Heart Diseases Antibodies  >198 publications with IL18 and Heart Diseases
Organs/Tissues associated with IL18 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >351 publications with IL18 and Kidney
 Lung Antibodies  >314 publications with IL18 and Lung
 Spleen Antibodies  >281 publications with IL18 and Spleen
 Brain Antibodies  >209 publications with IL18 and Brain
 Connective Tissue Antibodies  >72 publications with IL18 and Connective Tissue
 Stomach Antibodies  >40 publications with IL18 and Stomach
 Eye Antibodies  >37 publications with IL18 and Eye
 Ovary Antibodies  >27 publications with IL18 and Ovary
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