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MPIF-1 elisa kit :: Monkey Myeloid Progenitor Inhibitory Factor 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS734515
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Myeloid Progenitor Inhibitory Factor 1 (MPIF-1), ELISA Kit

 Also Known As   

Monkey Myeloid Progenitor Inhibitory Factor 1 ELISA Kit

 Product Gene Name   

MPIF-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    602494
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 Species Reactivity    Monkey
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This MPIF-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey MPIF-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of MPIF-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MPIF-1 purchase    MBS734515 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myeloid Progenitor Inhibitory Factor 1 (MPIF-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MPIF-1, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS734515 kit is based on MPIF-1 antibody-MPIF-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MPIF-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MPIF-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for MPIF-1 elisa kit

   Principle of the Assay: MPIF-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MPIF-1 antibody and an MPIF-1-HRP conjugate. The assay sample and buffer are incubated together with MPIF-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MPIF-1 concentration since MPIF-1 from samples and MPIF-1-HRP conjugate compete for the anti-MPIF-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MPIF-1 from the sample, fewer sites are left to bind MPIF-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MPIF-1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for MPIF-1 elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of MPIF-1 elisa kit    MPIF-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS734515
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NCBI/Uniprot data below describe general gene information for MPIF-1. It may not necessarily be applicable to this product.
 NCBI GI #    1916250
 NCBI GeneID    6368
 NCBI Accession #    AAB51134.1 [Other Products]
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 UniProt Secondary Accession #    O00174; O75950; Q52LD4; B7ZKQ3 [Other Products]
 UniProt Related Accession #    P55773 [Other Products]
 Molecular Weight    15,290 Da
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 NCBI Official Full Name    MPIF-1
 NCBI Official Synonym Full Names    chemokine (C-C motif) ligand 23
 NCBI Official Symbol    CCL23 [Similar Products]
 NCBI Official Synonym Symbols   
CKb8; MIP3; Ckb-8; MIP-3; MPIF-1; SCYA23; Ckb-8-1; hmrp-2a; CK-BETA-8
[Similar Products]
 NCBI Protein Information    C-C motif chemokine 23; C6 beta-chemokine; macrophage inflammatory protein 3; myeloid progenitor inhibitory factor 1; small inducible cytokine subfamily A (Cys-Cys), member 23
 UniProt Protein Name    C-C motif chemokine 23
 UniProt Synonym Protein Names   
CK-beta-8; CKB-8; Macrophage inflammatory protein 3; MIP-3; Myeloid progenitor inhibitory factor 1; MPIF-1; Small-inducible cytokine A23Cleaved into the following 4 chains:CCL23(19-99); CCL23(22-99); CCL23(27-99); CCL23(30-99)
 UniProt Gene Name    CCL23 [Similar Products]
 UniProt Synonym Gene Names    MIP3; MPIF1; SCYA23; CKB-8; MIP-3; MPIF-1 [Similar Products]
 UniProt Entry Name    CCL23_HUMAN
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 NCBI Summary for MPIF-1    This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. In addition, the product of this gene is a potent agonist of the chemokine (C-C motif) receptor 1. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]
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 UniProt Comments for MPIF-1    CCL6: Shows chemotactic activity for monocytes, resting T- lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; chemokine activity

Biological Process: cellular calcium ion homeostasis; negative regulation of cell proliferation; monocyte chemotaxis; G-protein coupled receptor protein signaling pathway; cell-cell signaling; immune response; chemotaxis; inflammatory response; signal transduction
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 Research Articles on MPIF-1    1. ADAMTS 8, CCL23, and TNFSF15 are implicated in anti-angiogenic activities
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Monkey Myeloid Progenitor Inhibitory Factor 1 ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS2089538  Myeloid Progenitor Inhibitory Factor 1 (MPIF1) Mini Samples ELISA Kit  MBS453133  Human Myeloid Progenitor Inhibitory Factor 1 (MPIF1) ELISA Kit
 MBS2004368  Polyclonal Antibody to Myeloid Progenitor Inhibitory Factor 1 (MPIF1)  MBS016829  Fish Myeloid Progenitor Inhibitory Factor 1 ELISA Kit
 MBS2056714  FITC-Linked Polyclonal Antibody to Myeloid Progenitor Inhibitory Factor 1 (MPIF1)  MBS262926  Chicken Myeloid Progenitor Inhibitory Factor 1 (MPIF-1) ELISA Kit
 MBS066947  Camel Myeloid Progenitor Inhibitory Factor 1 ELISA Kit  MBS2056712  APC-Linked Polyclonal Antibody to Myeloid Progenitor Inhibitory Factor 1 (MPIF1)
 MBS020890  Rat Myeloid Progenitor Inhibitory Factor 1 ELISA Kit  MBS067359  Duck Myeloid Progenitor Inhibitory Factor 1 ELISA Kit
Products associated with MPIF-1 elisa kitPathways associated with MPIF-1 elisa kit
 Reference Product  PubMed Publications
 CCR1 elisa kit  >17 publications with MPIF-1 and CCR1
 CCR2 elisa kit  >5 publications with MPIF-1 and CCR2
 CCR3 elisa kit  >4 publications with MPIF-1 and CCR3
 CXCR4 elisa kit  >3 publications with MPIF-1 and CXCR4
 CCR5 elisa kit  >3 publications with MPIF-1 and CCR5
 CX3CR1 elisa kit  >2 publications with MPIF-1 and CX3CR1
 Products by Pathway  Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway antibodies  Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
Diseases associated with MPIF-1 elisa kitOrgans/Tissues associated with MPIF-1 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >7 publications with MPIF-1 and Carcinoma
 Cardiovascular Diseases Antibodies  >6 publications with MPIF-1 and Cardiovascular Diseases
 Premature Birth Antibodies  >3 publications with MPIF-1 and Premature Birth
 Obstetric Labor, Premature Antibodies  >3 publications with MPIF-1 and Obstetric Labor, Premature
 Leukemia, Myeloid, Acute Antibodies  >3 publications with MPIF-1 and Leukemia, Myeloid, Acute
 Heart Diseases Antibodies  >2 publications with MPIF-1 and Heart Diseases
 Fetal Resorption Antibodies  >1 publications with MPIF-1 and Fetal Resorption
 Bone Neoplasms Antibodies  >1 publications with MPIF-1 and Bone Neoplasms
 Fatty Liver Antibodies  >1 publications with MPIF-1 and Fatty Liver
 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >2 publications with MPIF-1 and Placenta
 Liver Antibodies  >2 publications with MPIF-1 and Liver
 Lung Antibodies  >1 publications with MPIF-1 and Lung
 Uncharacterized Tissue Antibodies  >1 publications with MPIF-1 and Uncharacterized Tissue
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