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| Product Name
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P-Cadherin, ELISA Kit
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| Also Known As
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Monkey P-Cadherin (Cadherin, Placental) ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| OMIM
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114021
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| 3D Structure
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ModBase 3D Structure for P22223
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| Species Reactivity
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Monkey
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| Specificity
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This kit recognizes natural and recombinant Monkey P-Cadherin. No significant cross-reactivity or interference between Monkey P-Cadherin and analogues was observed.
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| Samples
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Serum, plasma and other biological fluids.
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| Assay Type
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Sandwich
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| Detection Range
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0.156-10ng/mL
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| Sensitivity
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0.094ng/mL
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| Preparation and Storage
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Store at 4 degree C.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of P-Cadherin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for P-Cadherin purchase
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MBS2505158 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the P-Cadherin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing P-Cadherin, with an estimated sensitivity of 0.094ng/mL. The ELISA analytical biochemical technique of the MBS2505158 kit is based on P-Cadherin antibody-P-Cadherin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect P-Cadherin antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, P-Cadherin. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey P-Cadherin concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to P-Cadherin. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for P-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain P-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of P-Cadherin. You can calculate the concentration of P-Cadherin in the samples by comparing the OD of the samples to the standard curve.
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| Sample Manual Insert of MBS2505158. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for P-Cadherin. It may not necessarily be applicable to this product. |
| NCBI GI #
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2950172
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| NCBI GeneID
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1001
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| NCBI Accession #
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CAA65093.1
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[Other Products]
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| UniProt Primary Accession #
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P22223
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[Other Products]
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| UniProt Secondary Accession #
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Q05DI6; B2R6F4
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[Other Products]
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| UniProt Related Accession #
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P22223
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[Other Products]
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| Molecular Weight
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86,552 Da
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| NCBI Official Full Name
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P-cadherin, partial
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| NCBI Official Synonym Full Names
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cadherin 3, type 1, P-cadherin (placental)
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| NCBI Official Symbol
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CDH3
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[Similar Products]
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| NCBI Official Synonym Symbols
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CDHP; HJMD; PCAD
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[Similar Products]
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| NCBI Protein Information
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cadherin-3; calcium-dependent adhesion protein, placental
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| UniProt Protein Name
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Cadherin-3
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| UniProt Synonym Protein Names
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Placental cadherin; P-cadherin
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| UniProt Gene Name
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CDH3
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[Similar Products]
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| UniProt Synonym Gene Names
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CDHP; P-cadherin
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[Similar Products]
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| UniProt Entry Name
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CADH3_HUMAN
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| NCBI Summary for P-Cadherin
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This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. [provided by RefSeq, Jul 2008]
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| UniProt Comments for P-Cadherin
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CDH3: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Interacts with CDCP1. Expressed in some normal epithelial tissues and in some carcinoma cell lines. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: cell-cell adherens junction; cytoplasm; plasma membrane; integral to membrane
Molecular Function: calcium ion binding
Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; response to drug; intercellular junction assembly and maintenance; wound healing; positive regulation of melanin biosynthetic process; Wnt receptor signaling pathway through beta-catenin; hair cycle process; retinal homeostasis; negative regulation of transforming growth factor-beta2 production; cell-cell adhesion; negative regulation of catagen; positive regulation of monophenol monooxygenase activity; visual perception; keratinization; cell adhesion; homophilic cell adhesion
Disease: Ectodermal Dysplasia, Ectrodactyly, And Macular Dystrophy Syndrome; Hypotrichosis, Congenital, With Juvenile Macular Dystrophy
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| Research Articles on P-Cadherin
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1. Positive N-cadherin and P-cadherin expression closely correlated with clinicopathological and biological behaviors, and poor-prognosis of gallbladder cancer.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with P-Cadherin elisa kit | Pathways associated with P-Cadherin elisa kit |
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| Diseases associated with P-Cadherin elisa kit | Organs/Tissues associated with P-Cadherin elisa kit |
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ELISA Kit 
