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| Product Name
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PSA/KLK3, ELISA Kit ★Popular Item★
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| Also Known As
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Monkey PSA/KLK3 (Prostate Specific Antigen) ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| MBS2503876 COA
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| OMIM
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176820
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| 3D Structure
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ModBase 3D Structure for P07288
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| Species Reactivity
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Monkey
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| Specificity
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This kit recognizes natural and recombinant Monkey PSA/KLK3. No significant cross-reactivity or interference between Monkey PSA/KLK3 and analogues was observed.
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| Samples
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Serum, plasma and other biological fluids.
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| Assay Type
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Sandwich
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| Detection Range
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0.313-20ng/mL
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| Sensitivity
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The minimum detectable dose of Monkey PSA/KLK3 is 0.188ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
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| Intended Uses
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This ELISA kit applies to the in vitro quantitative determination of Monkey PSA/KLK3 concentrations in serum, plasma and other biological fluids.
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| Preparation and Storage
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Store at 4 degree C.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of PSA/KLK3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for PSA/KLK3 purchase
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MBS2503876 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PSA/KLK3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing PSA/KLK3, with an estimated sensitivity of The minimum detectable dose of Monkey PSA/KLK3 is 0.188ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). The ELISA analytical biochemical technique of the MBS2503876 kit is based on PSA/KLK3 antibody-PSA/KLK3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PSA/KLK3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PSA/KLK3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PSA/KLK3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PSA/KLK3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PSA/KLK3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PSA/KLK3. You can calculate the concentration of PSA/KLK3 in the samples by comparing the OD of the samples to the standard curve.
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| Sample Manual Insert of MBS2503876. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for PSA/KLK3. It may not necessarily be applicable to this product. |
| NCBI GI #
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4502173
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| NCBI GeneID
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354
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| NCBI Accession #
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NP_001639.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_001648.2
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[Other Products]
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| UniProt Primary Accession #
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P07288
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[Other Products]
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| UniProt Secondary Accession #
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Q15096; Q16272; Q86TG8; Q8IXI4; C9JXH3; G3V0H4; G3XAE3
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[Other Products]
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| UniProt Related Accession #
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P07288
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[Other Products]
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| Molecular Weight
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25,116 Da
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| NCBI Official Full Name
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prostate-specific antigen isoform 1 preproprotein
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| NCBI Official Synonym Full Names
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kallikrein-related peptidase 3
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| NCBI Official Symbol
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KLK3
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[Similar Products]
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| NCBI Official Synonym Symbols
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APS; PSA; hK3; KLK2A1
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[Similar Products]
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| NCBI Protein Information
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prostate-specific antigen; P-30 antigen; gamma-seminoprotein; kallikrein-3; prostate specific antigen; semenogelase; seminin
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| UniProt Protein Name
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Prostate-specific antigen
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| UniProt Synonym Protein Names
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Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase
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| UniProt Gene Name
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KLK3
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[Similar Products]
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| UniProt Synonym Gene Names
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APS; PSA; Seminin
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[Similar Products]
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| UniProt Entry Name
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KLK3_HUMAN
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| NCBI Summary for PSA/KLK3
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Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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| UniProt Comments for PSA/KLK3
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PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.
Protein type: EC 3.4.21.77; Secreted; Protease; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19q13.41
Cellular Component: extracellular region; nucleus
Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis
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| Research Articles on PSA/KLK3
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1. Suggest that the occurrence of PCa is related to the genotype of KLK3 SNPs rs2735839.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with PSA/KLK3 elisa kit | Pathways associated with PSA/KLK3 elisa kit |
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| Diseases associated with PSA/KLK3 elisa kit | Organs/Tissues associated with PSA/KLK3 elisa kit |
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ELISA Kit 
