Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow PRG4 rightarrow Monkey PRG4  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • SIRT3 Antibody
 • CD88-C5aR (Phospho-Ser338) Antibody
 • ERAS Antibody
 • P210 ELISA Kit
 • Immunoglobulin G1 (IgG1) ELISA Kit
 • Hepatocyte Nuclear Factor 6 Beta (HNF6beta) ELISA Kit
 • Growth Factor Receptor Bound Protein 10 (GRB10) ELISA Kit
 • Forkhead Box Protein P1 (FOXP1) ELISA Kit
 • Soluble Interleukin 2 Recepter (IL-2SR) ELISA Kit
 • Signal Transducer and Activator of Transcription 3 (STAT3) ELISA Kit
 • Cadherin, Epithelial (CDHE) ELISA Kit
 • Cathepsin L (CTSL) ELISA Kit
 • Endotoxin (EDT) ELISA Kit
 • Hexokinase (HK) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PRG4 elisa kit :: Monkey Proteoglycan 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS745971
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Proteoglycan 4 (PRG4), ELISA Kit

 Also Known As   

Monkey Proteoglycan 4 ELISA Kit

 Product Gene Name   

PRG4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    604283
Section Bar
 Species Reactivity    Monkey
Section Bar
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Sensitivity    1.0 ng/mL.
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PRG4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PRG4 purchase    MBS745971 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proteoglycan 4 (PRG4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRG4, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS745971 kit is based on PRG4 antibody-PRG4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRG4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRG4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for PRG4 elisa kit

   Intended Uses: This PRG4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey PRG4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PRG4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PRG4. Standards or samples are then added to the microtiter plate wells and PRG4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PRG4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PRG4 are added to each well to "sandwich" the PRG4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PRG4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRG4 concentration in each sample is interpolated from this standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of PRG4 elisa kit    PRG4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS745971. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PRG4. It may not necessarily be applicable to this product.
 NCBI GI #    50345548
 NCBI Accession #    AAT74746.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q6DNC4; Q6DNC5; Q6ZMZ5; Q9BX49 [Other Products]
 Molecular Weight    146,468 Da
Table BarTOPTable Bar
 NCBI Official Full Name    proteoglycan 4, partial
 UniProt Protein Name    Proteoglycan 4
 UniProt Synonym Protein Names   
Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycanCleaved into the following chain:Proteoglycan 4 C-terminal part
 Protein Family    Proteoglycan
 UniProt Gene Name    PRG4 [Similar Products]
 UniProt Synonym Gene Names    MSF; SZP [Similar Products]
 UniProt Entry Name    PRG4_HUMAN
Section Bar
 UniProt Comments for PRG4    PRG4: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Defects in PRG4 are the cause of camptodactyly- arthropathy-coxa vara-pericarditis syndrome (CACP); also known as Jacobs syndrome. CACP is an autosomal recessive disorder. Individuals with CACP have normal appearing joints at birth but with advancing age develop joint failure associated with noninflammatory synoviocyte hyperplasia and subintimal fibrosis of the synovial capsule. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q25-q31

Cellular Component: extracellular space

Molecular Function: scavenger receptor activity; polysaccharide binding

Biological Process: cell proliferation; receptor-mediated endocytosis; immune response; negative regulation of interleukin-6 biosynthetic process; regulation of cell proliferation

Disease: Camptodactyly-arthropathy-coxa Vara-pericarditis Syndrome
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions