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TM elisa kit :: Monkey Thrombomodulin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS738224
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Thrombomodulin (TM), ELISA Kit

★Popular Item★
 Also Known As   

Monkey Thrombomodulin ELISA Kit

 Product Gene Name   

TM elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    614486
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 Species Reactivity    Monkey
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 Specificity    This assay has high sensitivity and excellent specificity for detection of TM. No significant cross-reactivity or interference between TM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TM and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    250-5000pg/mL
 Sensitivity    1.0pg/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TM purchase    MBS738224 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thrombomodulin (TM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 250-5000pg/mL in biological research samples containing TM, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS738224 kit is based on TM antibody-TM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TM elisa kit

   Intended Uses: This TM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey TM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||TM ELISA kit applies the competitive enzyme immunoassay technique `utilizing a monoclonal anti-TM antibody and an TM-HRP conjugate. The assay sample and buffer are incubated together with TM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TM concentration since TM from samples and TM-HRP conjugate compete for the anti-TM antibody binding site. Since the number of sites is limited, as more sites are occupied by TM from the sample, fewer sites are left to bind TM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TM concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for TM elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve of TM elisa kit    TM elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS738224. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TM. It may not necessarily be applicable to this product.
 NCBI GI #    339657
 NCBI GeneID    7056
 NCBI Accession #    AAB59508.1 [Other Products]
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 UniProt Secondary Accession #    Q8IV29; Q9UC32 [Other Products]
 UniProt Related Accession #    P07204 [Other Products]
 Molecular Weight    60,329 Da
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 NCBI Official Full Name    thrombomodulin
 NCBI Official Synonym Full Names    thrombomodulin
 NCBI Official Symbol    THBD [Similar Products]
 NCBI Official Synonym Symbols   
TM; THRM; AHUS6; BDCA3; CD141; THPH12
[Similar Products]
 NCBI Protein Information    thrombomodulin; fetomodulin; CD141 antigen
 UniProt Protein Name    Thrombomodulin
 UniProt Synonym Protein Names   
Fetomodulin; CD_antigen: CD141
 Protein Family    Thrombomodulin
 UniProt Gene Name    THBD [Similar Products]
 UniProt Synonym Gene Names    THRM; TM [Similar Products]
 UniProt Entry Name    TRBM_HUMAN
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 NCBI Summary for TM    The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TM    thrombomodulin: Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THPH12). A hemostatic disorder characterized by a tendency to thrombosis. Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p11.2

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; calcium ion binding; receptor activity

Biological Process: negative regulation of fibrinolysis; response to cAMP; negative regulation of blood coagulation; response to lipopolysaccharide; female pregnancy; signal transduction; blood coagulation; leukocyte migration; response to X-ray

Disease: Thrombophilia Due To Thrombomodulin Defect; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 6
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 Research Articles on TM    1. TM 1418C/T polymorphism is independent predictor of AMI and synergies with smoking.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TM elisa kitPathways associated with TM elisa kit
 Reference Product  PubMed Publications
 JUN elisa kit  >21 publications with TM and JUN
 PROCR elisa kit  >8 publications with TM and PROCR
 CRP elisa kit  >3 publications with TM and CRP
 PROC elisa kit  >3 publications with TM and PROC
 Products by Pathway  Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway antibodies  Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway Diagram
Diseases associated with TM elisa kitOrgans/Tissues associated with TM elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >43 publications with TM and Cardiovascular Diseases
 Inflammation Antibodies  >34 publications with TM and Inflammation
 Thrombosis Antibodies  >27 publications with TM and Thrombosis
 Thrombophilia Antibodies  >19 publications with TM and Thrombophilia
 Kidney Diseases Antibodies  >19 publications with TM and Kidney Diseases
 Disseminated Intravascular Coagulation Antibodies  >15 publications with TM and Disseminated Intravascular Coagulation
 Atypical hemolytic uremic syndrome Antibodies  >9 publications with TM and Atypical hemolytic uremic syndrome
 Nervous System Diseases Antibodies  >9 publications with TM and Nervous System Diseases
 Heart Diseases Antibodies  >9 publications with TM and Heart Diseases
 Lung Diseases Antibodies  >8 publications with TM and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >87 publications with TM and Blood
 Vascular Antibodies  >39 publications with TM and Vascular
 Kidney Antibodies  >13 publications with TM and Kidney
 Liver Antibodies  >10 publications with TM and Liver
 Lung Antibodies  >10 publications with TM and Lung
 Brain Antibodies  >8 publications with TM and Brain
 Bone Antibodies  >7 publications with TM and Bone
 Heart Antibodies  >7 publications with TM and Heart
 Embryonic Tissue Antibodies  >6 publications with TM and Embryonic Tissue
 Stomach Antibodies  >5 publications with TM and Stomach
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