|
| Product Name
|
|
Transferrin Receptor (TFR), ELISA Kit
|
| Also Known As
|
|
Monkey Transferrin Receptor ELISA Kit
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
| Sample Manual Insert
|
|
 
|
| Request for Current Manual Insert
|
|
 |
|
| OMIM
|
|
190010
|
|
| Species Reactivity
|
|
Monkey
|
|
|
|
|
| Samples
|
|
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
|
| Assay Type
|
|
Sandwich
|
| Detection Range
|
|
2.5-50ng/mL
|
| Sensitivity
|
|
1.0ng/mL
|
| Intended Uses
|
|
This TFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey TFR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
|
|
|
| Preparation and Storage
|
|
Store all reagents at 2-8 degree C.
|
| Sample Preparation
|
|
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
|
| Product Note
|
|
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
|
| Other Notes
|
|
Small volumes of TFR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
| Searchable Terms for TFR purchase
|
|
MBS735274 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transferrin Receptor (TFR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50ng/mL in biological research samples containing TFR, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS735274 kit is based on TFR antibody-TFR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TFR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TFR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
|
|
TOP
|
|
Related Product Information for TFR elisa kit
|
|
Principle of the Assay: TFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TFR antibody and an TFR-HRP conjugate. The assay sample and buffer are incubated together with TFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFR concentration since TFR from samples and TFR-HRP conjugate compete for the anti-TFR antibody binding site. Since the number of sites is limited, as more sites are occupied by TFR from the sample, fewer sites are left to bind TFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFR concentration in each sample is interpolated from this standard curve.
|
| Product Categories/Family for TFR elisa kit
|
|
Cardiovascular
|
|
|
|
| Typical Testing Data/Standard Curve (for reference only) of TFR elisa kit
|
|
|
|
TOP
|
|
| Sample Manual Insert of MBS735274. Click to request current manual
|
|
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for TFR. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
339516
|
| NCBI GeneID
|
|
7037
|
| NCBI Accession #
|
|
AAA61153.1
|
[Other Products]
|
|
|
| UniProt Secondary Accession #
|
|
Q1HE24; Q59G55; Q9UCN0; Q9UCU5; Q9UDF9; Q9UK21; D3DXB0
|
[Other Products]
|
| UniProt Related Accession #
|
|
P02786
|
[Other Products]
|
| Molecular Weight
|
|
84,871 Da
|
|
TOP
|
| NCBI Official Full Name
|
|
transferrin receptor
|
| NCBI Official Synonym Full Names
|
|
transferrin receptor
|
| NCBI Official Symbol
|
|
TFRC
|
[Similar Products]
|
| NCBI Official Synonym Symbols
|
|
T9; TR; TFR; p90; CD71; TFR1; TRFR
|
[Similar Products]
|
| NCBI Protein Information
|
|
transferrin receptor protein 1; transferrin receptor (p90, CD71)
|
| UniProt Protein Name
|
|
Transferrin receptor protein 1
|
| UniProt Synonym Protein Names
|
|
T9; p90; CD_antigen: CD71Cleaved into the following chain:Transferrin receptor protein 1, serum form; sTfR
|
| Protein Family
|
|
Transferrin receptor protein
|
| UniProt Gene Name
|
|
TFRC
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
TR; TfR; TfR1; Trfr; sTfR
|
[Similar Products]
|
| UniProt Entry Name
|
|
TFR1_HUMAN
|
|
|
| UniProt Comments for TFR
|
|
TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system.
Protein type: Receptor, misc.; Membrane protein, integral; Cell surface
Chromosomal Location of Human Ortholog: 3q29
Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; membrane; perinuclear region of cytoplasm; plasma membrane; melanosome; endosome; vesicle; external side of plasma membrane
Molecular Function: identical protein binding; protein binding; transferrin receptor activity; double-stranded RNA binding
Biological Process: receptor-mediated endocytosis; viral reproduction; cellular iron ion homeostasis; positive regulation of bone resorption; transferrin transport; regulation of cell growth; osteoclast differentiation; transmembrane transport; regulation of cell proliferation
|
|
TOP
|
|
| Research Articles on TFR
|
|
1. results suggest that Transferrin or a TF-TFR2 complex may have a role in the etiology of PD, possibly through iron misregulation or mitochondrial dysfunction within dopaminergic neurons.
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Products associated with TFR elisa kit | Pathways associated with TFR elisa kit |
|
|
|
| Diseases associated with TFR elisa kit | Organs/Tissues associated with TFR elisa kit |
|
|
|
|
|
|
TOP
|
ELISA Kit 
