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A-Glu elisa kit :: Monkey alpha-Glucosidase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS744987
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alpha-Glucosidase, ELISA Kit

★Popular Item★
 Also Known As   

Monkey alpha-Glucosidase ELISA Kit

 Product Synonym Names    Monkey a-Glucosidase ELISA Kit
 Product Gene Name   

A-Glu elisa kit

[Similar Products]
 Product Synonym Gene Name    A-Glu [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606800
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 Species Reactivity    Monkey
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 Specificity    This assay has high sensitivity and excellent specificity for detection of AGLU. No significant cross-reactivity or interference between AGLU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGLU and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    25-500ng/mL
 Sensitivity    1.0ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of A-Glu elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for A-Glu purchase    MBS744987 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-Glucosidase, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25-500ng/mL in biological research samples containing A-Glu, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS744987 kit is based on A-Glu antibody-A-Glu antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect A-Glu antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, A-Glu. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for A-Glu elisa kit

   Intended Uses: This AGLU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey AGLU.

Principle of the Assay: AGLU ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGLU antibody and an AGLU-HRP conjugate. The assay sample and buffer are incubated together with AGLU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGLU concentration since AGLU from samples and AGLU-HRP conjugate compete for the anti-AGLU antibody binding site. Since the number of sites is limited, as more sites are occupied by AGLU from the sample, fewer sites are left to bind AGLU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGLU concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of A-Glu elisa kit    A-Glu elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS744987. Click to request current manual
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NCBI/Uniprot data below describe general gene information for A-Glu. It may not necessarily be applicable to this product.
 NCBI GI #    114159823
 NCBI GeneID    2548
 NCBI Accession #    ABI53718.1 [Other Products]
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 UniProt Secondary Accession #    Q09GN4; Q14351; Q16302; Q8IWE7 [Other Products]
 UniProt Related Accession #    P10253 [Other Products]
 Molecular Weight    105,324 Da
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 NCBI Official Full Name    alpha-glucosidase
 NCBI Official Synonym Full Names    glucosidase, alpha; acid
 NCBI Official Symbol    GAA [Similar Products]
 NCBI Official Synonym Symbols   
LYAG
[Similar Products]
 NCBI Protein Information    lysosomal alpha-glucosidase; acid maltase; aglucosidase alfa
 UniProt Protein Name    Lysosomal alpha-glucosidase
 UniProt Synonym Protein Names   
Acid maltase; Aglucosidase alfaCleaved into the following 2 chains:76 kDa lysosomal alpha-glucosidase; 70 kDa lysosomal alpha-glucosidase
 Protein Family    Alpha-glucosidase
 UniProt Gene Name    GAA [Similar Products]
 UniProt Entry Name    LYAG_HUMAN
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 NCBI Summary for A-Glu    This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for A-Glu    GAA: Essential for the degradation of glygogen to glucose in lysosomes. Defects in GAA are the cause of glycogen storage disease type 2 (GSD2); also called acid alpha-glucosidase (GAA) deficiency or acid maltase deficiency (AMD). GSD2 is a metabolic disorder with a broad clinical spectrum. The severe infantile form, or Pompe disease, presents at birth with massive accumulation of glycogen in muscle, heart and liver. Cardiomyopathy and muscular hypotonia are the cardinal features of this form whose life expectancy is less than two years. The juvenile and adult forms present as limb-girdle muscular dystrophy beginning in the lower limbs. Final outcome depends on respiratory muscle failure. Patients with the adult form can be free of clinical symptoms for most of their life but finally develop a slowly progressive myopathy. Belongs to the glycosyl hydrolase 31 family.

Protein type: Contractile; Hydrolase; Carbohydrate Metabolism - galactose; EC 3.2.1.20; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 17q25.2-q25.3

Cellular Component: membrane; lysosome; lysosomal membrane

Molecular Function: alpha-glucosidase activity; maltase activity; carbohydrate binding

Biological Process: heart morphogenesis; maltose metabolic process; tissue development; glycogen catabolic process; vacuolar sequestering; glucose metabolic process; sucrose metabolic process; locomotory behavior; muscle maintenance; neuromuscular process controlling posture; lysosome organization and biogenesis; diaphragm contraction; neuromuscular process controlling balance; regulation of the force of heart contraction; cardiac muscle contraction

Disease: Glycogen Storage Disease Ii
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 Research Articles on A-Glu    1. The phenotype LO-GSDII with GAA mutation in the North of Italy seems not significantly different from other LO-GSDII populations in Europe or the USA.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with A-Glu elisa kitPathways associated with A-Glu elisa kit
 Reference Product  PubMed Publications
 AGA elisa kit  >27 publications with A-Glu and AGA
 GLA elisa kit  >8 publications with A-Glu and GLA
 YY1 elisa kit  >2 publications with A-Glu and YY1
 MGAM elisa kit  >1 publications with A-Glu and MGAM
 AGL elisa kit  >1 publications with A-Glu and AGL
 Products by Pathway  Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram
 Starch And Sucrose Metabolism Pathway antibodies  Starch And Sucrose Metabolism Pathway Diagram
 Starch And Sucrose Metabolism Pathway antibodies  Starch And Sucrose Metabolism Pathway Diagram
Diseases associated with A-Glu elisa kitOrgans/Tissues associated with A-Glu elisa kit
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >732 publications with A-Glu and Brain Diseases
 Glycogen Storage Disease Type II Antibodies  >339 publications with A-Glu and Glycogen Storage Disease Type II
 Cardiovascular Diseases Antibodies  >123 publications with A-Glu and Cardiovascular Diseases
 Cardiomyopathy, Hypertrophic Antibodies  >54 publications with A-Glu and Cardiomyopathy, Hypertrophic
 Liver Neoplasms Antibodies  >43 publications with A-Glu and Liver Neoplasms
 Kidney Diseases Antibodies  >42 publications with A-Glu and Kidney Diseases
 Atrophy Antibodies  >41 publications with A-Glu and Atrophy
 Hypertrophy Antibodies  >34 publications with A-Glu and Hypertrophy
 Hypertrophy, Left Ventricular Antibodies  >15 publications with A-Glu and Hypertrophy, Left Ventricular
 Fibrosis Antibodies  >13 publications with A-Glu and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >345 publications with A-Glu and Blood
 Muscle Antibodies  >317 publications with A-Glu and Muscle
 Brain Antibodies  >149 publications with A-Glu and Brain
 Kidney Antibodies  >62 publications with A-Glu and Kidney
 Skin Antibodies  >62 publications with A-Glu and Skin
 Vascular Antibodies  >49 publications with A-Glu and Vascular
 Pancreas Antibodies  >44 publications with A-Glu and Pancreas
 Bone Antibodies  >34 publications with A-Glu and Bone
 Lung Antibodies  >29 publications with A-Glu and Lung
 Embryonic Tissue Antibodies  >16 publications with A-Glu and Embryonic Tissue
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