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cPLA2 elisa kit :: Monkey cPLA2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2503779 cPLA2 elisa kit
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cPLA2, ELISA Kit

 Also Known As   

Monkey cPLA2 (Cytosolic Phospholipase A2) ELISA Kit

 Product Gene Name   

cPLA2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    600522
 3D Structure    ModBase 3D Structure for P47712
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 Species Reactivity    Monkey
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 Specificity    This kit recognizes natural and recombinant Monkey cPLA2. No significant cross-reactivity or interference between Monkey cPLA2 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.094ng/mL
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 Preparation and Storage    Store at 4 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of cPLA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for cPLA2 purchase    MBS2503779 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cPLA2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing cPLA2, with an estimated sensitivity of 0.094ng/mL. The ELISA analytical biochemical technique of the MBS2503779 kit is based on cPLA2 antibody-cPLA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect cPLA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, cPLA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for cPLA2 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey cPLA2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to cPLA2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for cPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain cPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of cPLA2. You can calculate the concentration of cPLA2 in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2503779
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NCBI/Uniprot data below describe general gene information for cPLA2. It may not necessarily be applicable to this product.
 NCBI GI #    23943920
 NCBI GeneID    5321
 NCBI Accession #    NP_077734.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_024420.2 [Other Products]
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 UniProt Primary Accession #    P47712 [Other Products]
 UniProt Secondary Accession #    Q29R80; B1AKG4 [Other Products]
 UniProt Related Accession #    P47712 [Other Products]
 Molecular Weight    85,239 Da
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 NCBI Official Full Name    cytosolic phospholipase A2
 NCBI Official Synonym Full Names    phospholipase A2, group IVA (cytosolic, calcium-dependent)
 NCBI Official Symbol    PLA2G4A [Similar Products]
 NCBI Official Synonym Symbols   
PLA2G4; cPLA2-alpha
[Similar Products]
 NCBI Protein Information    cytosolic phospholipase A2; cPLA2; calcium-dependent phospholipid-binding protein; lysophospholipase; phosphatidylcholine 2-acylhydrolase; phospholipase A2 group IVA
 UniProt Protein Name    Cytosolic phospholipase A2
 UniProt Synonym Protein Names   
Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase
 UniProt Gene Name    PLA2G4A [Similar Products]
 UniProt Synonym Gene Names    CPLA2; PLA2G4; cPLA2 [Similar Products]
 UniProt Entry Name    PA24A_HUMAN
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 NCBI Summary for cPLA2    This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq, Jul 2008]
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 UniProt Comments for cPLA2    cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A.

Protein type: Lipid Metabolism - ether lipid; Phospholipase; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - linoleic acid; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4; Lipid Metabolism - arachidonic acid; EC 3.1.1.5

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cytoplasmic membrane-bound vesicle; mitochondrial inner membrane; cytoplasm; cytosol

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; calcium ion binding; lysophospholipase activity

Biological Process: icosanoid biosynthetic process; platelet activation; platelet activating factor biosynthetic process; phospholipid catabolic process; phospholipid metabolic process; icosanoid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; arachidonic acid metabolic process; arachidonic acid secretion; blood coagulation; regulation of cell proliferation
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 Research Articles on cPLA2    1. Data indicate 3-(1-Aryl-1H-indol-5-yl)propanoic acids a potent inhibitor of cytosolic phospholipase A2alpha (cPLA2alpha).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with cPLA2 elisa kitPathways associated with cPLA2 elisa kit
 Reference Product  PubMed Publications
 PTGS2 elisa kit  >308 publications with cPLA2 and PTGS2
 PLA2G6 elisa kit  >108 publications with cPLA2 and PLA2G6
 PTGS1 elisa kit  >96 publications with cPLA2 and PTGS1
 ALOX5 elisa kit  >62 publications with cPLA2 and ALOX5
 SYK elisa kit  >8 publications with cPLA2 and SYK
 PLD2 elisa kit  >4 publications with cPLA2 and PLD2
 KAT5 elisa kit  >2 publications with cPLA2 and KAT5
 MKNK1 elisa kit  >1 publications with cPLA2 and MKNK1
 Products by Pathway  Pathway Diagram
 ADP Signalling Through P2Y Purinoceptor 1 Pathway antibodies  ADP Signalling Through P2Y Purinoceptor 1 Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Acyl Chain Remodeling Of CL Pathway antibodies  Acyl Chain Remodeling Of CL Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Acyl Chain Remodelling Of PE Pathway antibodies  Acyl Chain Remodelling Of PE Pathway Diagram
 Acyl Chain Remodelling Of PG Pathway antibodies  Acyl Chain Remodelling Of PG Pathway Diagram
 Acyl Chain Remodelling Of PI Pathway antibodies  Acyl Chain Remodelling Of PI Pathway Diagram
 Acyl Chain Remodelling Of PS Pathway antibodies  Acyl Chain Remodelling Of PS Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
Diseases associated with cPLA2 elisa kitOrgans/Tissues associated with cPLA2 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >314 publications with cPLA2 and Inflammation
 Necrosis Antibodies  >175 publications with cPLA2 and Necrosis
 Nervous System Diseases Antibodies  >147 publications with cPLA2 and Nervous System Diseases
 Disease Models, Animal Antibodies  >121 publications with cPLA2 and Disease Models, Animal
 Lung Neoplasms Antibodies  >35 publications with cPLA2 and Lung Neoplasms
 Edema Antibodies  >35 publications with cPLA2 and Edema
 Heart Diseases Antibodies  >33 publications with cPLA2 and Heart Diseases
 Neoplasms, Experimental Antibodies  >31 publications with cPLA2 and Neoplasms, Experimental
 Liver Diseases Antibodies  >29 publications with cPLA2 and Liver Diseases
 Pain Antibodies  >27 publications with cPLA2 and Pain
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >284 publications with cPLA2 and Brain
 Vascular Antibodies  >216 publications with cPLA2 and Vascular
 Lung Antibodies  >200 publications with cPLA2 and Lung
 Kidney Antibodies  >145 publications with cPLA2 and Kidney
 Embryonic Tissue Antibodies  >64 publications with cPLA2 and Embryonic Tissue
 Ovary Antibodies  >46 publications with cPLA2 and Ovary
 Eye Antibodies  >45 publications with cPLA2 and Eye
 Uterus Antibodies  >42 publications with cPLA2 and Uterus
 Skin Antibodies  >37 publications with cPLA2 and Skin
 Intestine Antibodies  >36 publications with cPLA2 and Intestine
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