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Add To Compare List DVD/DHVD 3 elisa kit :: Mouse 1, 25-dihydroxyvitamin D3 ELISA Kit Catalog # MBS731103 Unit / Price 48-Strip-Wells / $495 +1 FREE 8GB USB 96-Strip-Wells / $700 +1 FREE 8GB USB 5x96-Strip-Wells / $3,105 +3 FREE 8GB USB 10x96-Strip-Wells / $5,865 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name 1, 25-dihydroxyvitamin D3 (DVD/DHVD 3), ELISA Kit Popular Item Full Product Name Mouse 1, 25-dihydroxyvitamin D3 ELISA Kit Product Synonym Names Mouse 1,25-dihydroxyvitamin D3 (1,25OHVD3) ELISA Kit; Mouse 1, 25-dihydroxyvitamin D3 ELISA Kit; 1, 25-dihydroxyvitamin D3; 1, 25-dihydroxyvitamin D3 (Mouse) Product Gene Name DVD/DHVD 3 elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual MBS731103 MSDS MSDS PDF Species Reactivity Mouse Specificity This assay has high sensitivity and excellent specificity for detection of 1,25OHVD3. No significant cross-reactivity or interference between 1,25OHVD3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 1,25OHVD3 and all the analogues, therefore, cross reaction may still exist in some cases. Samples Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Assay Type Quantitative Competitive Sensitivity 1.0 pg/mL Preparation and Storage Store all reagents at 2-8 degree C Sample Preparation We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team ISO Certification Manufactured in an ISO 13485:2016 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of DVD/DHVD 3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for DVD/DHVD 3 purchase MBS731103 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1, 25-dihydroxyvitamin D3 (DVD/DHVD 3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DVD/DHVD 3. The ELISA analytical biochemical technique of the MBS731103 kit is based on DVD/DHVD 3 antibody-DVD/DHVD 3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DVD/DHVD 3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DVD/DHVD 3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for DVD/DHVD 3 elisa kit Intended Uses: This 1,25OHVD3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse 1,25OHVD3. This ELISA kit for research use only, not for therapeutic or test applications!Principle of the Assay: 1,25OHVD3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-1,25OHVD3 antibody and an 1,25OHVD3-HRP conjugate. The assay sample and buffer are incubated together with 1,25OHVD3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 1,25OHVD3 concentration since 1,25OHVD3 from samples and 1,25OHVD3-HRP conjugate compete for the anti-1,25OHVD3 antibody binding site. Since the number of sites is limited, as more sites are occupied by 1,25OHVD3 from the sample, fewer sites are left to bind 1,25OHVD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 1,25OHVD3 concentration in each sample is interpolated from this standard curve. Product Categories/Family for DVD/DHVD 3 elisa kit Mouse ELISA Kit Typical Testing Data/Standard Curve (for reference only) of DVD/DHVD 3 elisa kit TOP Sample Manual Insert of MBS731103. Product References and Citations for DVD/DHVD 3 elisa kit DMP-1-mediated Ghr gene recombination compromises skeletal development and impairs skeletal response to intermittent PTH. Zhongbo Liu, Oran D. Kennedy, Luis Cardoso, Jelena Basta-Pljakic, Nicola C. Partridge, Mitchell B. Schaffler, Clifford J. Rosen and Shoshana Yakar (2015) TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP