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HSD11b1 elisa kit :: Mouse 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2025514 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
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10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1), ELISA Kit

 Also Known As   

11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ELISA Kit

 Product Synonym Names    11DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; SDR26C1; Corticosteroid 11-beta-dehydrogenase isozyme 1; Short Chain Dehydrogenase/Reductase Family 26C,Member 1
 Product Gene Name   

HSD11b1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600713
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1).
No significant cross-reactivity or interference between 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.115ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HSD11b1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HSD11b1 purchase    MBS2025514 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing HSD11b1, with an estimated sensitivity of < 0.115ng/mL. The ELISA analytical biochemical technique of the MBS2025514 kit is based on HSD11b1 antibody-HSD11b1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSD11b1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSD11b1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HSD11b1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for HSD11b1 elisa kit    Enzyme & Kinase; Metabolic pathway; Endocrinology; Cardiovascular biology; Neuroscience; Hepatology; Hormone metabolism
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 Typical Testing Data/Standard Curve (for reference only) of HSD11b1 elisa kit    HSD11b1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2025514. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HSD11b1. It may not necessarily be applicable to this product.
 NCBI GI #    15146338
 NCBI GeneID    3290
 NCBI Accession #    AAK83653.1 [Other Products]
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 UniProt Secondary Accession #    B2R9Z1; D3DT89 [Other Products]
 UniProt Related Accession #    P28845 [Other Products]
 Molecular Weight    32,401 Da
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 NCBI Official Full Name    11-beta hydroxysteroid dehydrogenase type 1
 NCBI Official Synonym Full Names    hydroxysteroid (11-beta) dehydrogenase 1
 NCBI Official Symbol    HSD11B1 [Similar Products]
 NCBI Official Synonym Symbols   
HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1
[Similar Products]
 NCBI Protein Information    corticosteroid 11-beta-dehydrogenase isozyme 1
 UniProt Protein Name    Corticosteroid 11-beta-dehydrogenase isozyme 1
 UniProt Synonym Protein Names   
11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11-beta-HSD1; Short chain dehydrogenase/reductase family 26C member 1
 Protein Family    Corticosteroid 11-beta-dehydrogenase isozyme
 UniProt Gene Name    HSD11B1 [Similar Products]
 UniProt Synonym Gene Names    HSD11; HSD11L; SDR26C1; 11-DH; 11-beta-HSD1 [Similar Products]
 UniProt Entry Name    DHI1_HUMAN
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 NCBI Summary for HSD11b1    The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2011]
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 UniProt Comments for HSD11b1    HSD11B1: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7- ketocholesterol to 7-beta-hydroxycholesterol. Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD). In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Lipid Metabolism - C21-steroid hormone; EC 1.1.1.146; Endoplasmic reticulum; Membrane protein, integral; Oxidoreductase; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 1q32-q41

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: 11-beta-hydroxysteroid dehydrogenase activity

Biological Process: steroid metabolic process; glucocorticoid biosynthetic process; lung development

Disease: Cortisone Reductase Deficiency 2
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 Research Articles on HSD11b1    1. The polymorphism of HSD11B1 may be a cause of childhood obesity, or even associated with the complication of childhood obesity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HSD11b1 elisa kitPathways associated with HSD11b1 elisa kit
 Reference Product  PubMed Publications
 PPARG elisa kit  >6 publications with HSD11b1 and PPARG
 CYP11B2 elisa kit  >4 publications with HSD11b1 and CYP11B2
 CYP11B1 elisa kit  >4 publications with HSD11b1 and CYP11B1
 HSD3B1 elisa kit  >4 publications with HSD11b1 and HSD3B1
 HSD3B2 elisa kit  >3 publications with HSD11b1 and HSD3B2
 CYP2D6 elisa kit  >1 publications with HSD11b1 and CYP2D6
 AKR1D1 elisa kit  >1 publications with HSD11b1 and AKR1D1
 Products by Pathway  Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Glucocorticoid &amp; Mineralcorticoid Metabolism Pathway antibodies  Glucocorticoid &amp; Mineralcorticoid Metabolism Pathway Diagram
 Glucocorticoid Biosynthesis Pathway antibodies  Glucocorticoid Biosynthesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Steroid Hormones And Vitamin D Pathway antibodies  Metabolism Of Steroid Hormones And Vitamin D Pathway Diagram
Diseases associated with HSD11b1 elisa kitOrgans/Tissues associated with HSD11b1 elisa kit
 Disease Name  Pubmed Publications
 Obesity Antibodies  >87 publications with HSD11b1 and Obesity
 Insulin Resistance Antibodies  >54 publications with HSD11b1 and Insulin Resistance
 Hypertension Antibodies  >33 publications with HSD11b1 and Hypertension
 Cardiovascular Diseases Antibodies  >22 publications with HSD11b1 and Cardiovascular Diseases
 Obesity, Abdominal Antibodies  >19 publications with HSD11b1 and Obesity, Abdominal
 Liver Diseases Antibodies  >18 publications with HSD11b1 and Liver Diseases
 Inflammation Antibodies  >18 publications with HSD11b1 and Inflammation
 Weight Gain Antibodies  >13 publications with HSD11b1 and Weight Gain
 Necrosis Antibodies  >11 publications with HSD11b1 and Necrosis
 Cortisone reductase deficiency Antibodies  >11 publications with HSD11b1 and Cortisone reductase deficiency
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >80 publications with HSD11b1 and Blood
 Liver Antibodies  >62 publications with HSD11b1 and Liver
 Connective Tissue Antibodies  >52 publications with HSD11b1 and Connective Tissue
 Ovary Antibodies  >25 publications with HSD11b1 and Ovary
 Kidney Antibodies  >21 publications with HSD11b1 and Kidney
 Brain Antibodies  >19 publications with HSD11b1 and Brain
 Muscle Antibodies  >15 publications with HSD11b1 and Muscle
 Lung Antibodies  >13 publications with HSD11b1 and Lung
 Placenta Antibodies  >11 publications with HSD11b1 and Placenta
 Uterus Antibodies  >8 publications with HSD11b1 and Uterus
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