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YWHAG elisa kit :: Mouse 14-3-3 protein gamma (YWHAG) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7220089
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

14-3-3 protein gamma (YWHAG), ELISA Kit

 Also Known As   

Mouse 14-3-3 protein gamma (YWHAG) ELISA Kit

 Product Gene Name   

YWHAG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605356
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of YWHAG. No significant cross-reactivity or interference between YWHAG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between YWHAG and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    50-1000ng/mL
 Sensitivity    1.0ng/mL
 Intended Uses    This YWHAG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse YWHAG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of YWHAG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for YWHAG purchase    MBS7220089 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 14-3-3 protein gamma (YWHAG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000ng/mL in biological research samples containing YWHAG, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS7220089 kit is based on YWHAG antibody-YWHAG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect YWHAG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, YWHAG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for YWHAG elisa kit

   Principle of the assay: YWHAG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-YWHAG antibody and an YWHAG-HRP conjugate. The assay sample and buffer are incubated together with YWHAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YWHAG concentration since YWHAG from samples and YWHAG-HRP conjugate compete for the anti-YWHAG antibody binding site. Since the number of sites is limited, as more sites are occupied by YWHAG from the sample, fewer sites are left to bind YWHAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YWHAG concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for YWHAG elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of YWHAG elisa kit    YWHAG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7220089. Click to request current manual
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NCBI/Uniprot data below describe general gene information for YWHAG. It may not necessarily be applicable to this product.
 NCBI GI #    21464101
 NCBI GeneID    7532
 NCBI Accession #    NP_036611.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_012479.3 [Other Products]
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 UniProt Secondary Accession #    O70457; P35214; Q6FH52; Q9UDP2; Q9UN99 [Other Products]
 UniProt Related Accession #    P61981 [Other Products]
 Molecular Weight    28,303 Da [Similar Products]
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 NCBI Official Full Name    14-3-3 protein gamma
 NCBI Official Synonym Full Names    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma
 NCBI Official Symbol    YWHAG [Similar Products]
 NCBI Official Synonym Symbols   
PPP1R170; 14-3-3GAMMA
[Similar Products]
 NCBI Protein Information    14-3-3 protein gamma; KCIP-1; 14-3-3 gamma; protein kinase C inhibitor protein 1; protein phosphatase 1, regulatory subunit 170; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
 UniProt Protein Name    14-3-3 protein gamma
 UniProt Synonym Protein Names   
Protein kinase C inhibitor protein 1; KCIP-1
 Protein Family    14-3-3 protein
 UniProt Gene Name    YWHAG [Similar Products]
 UniProt Synonym Gene Names    KCIP-1 [Similar Products]
 UniProt Entry Name    1433G_HUMAN
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 NCBI Summary for YWHAG    This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]
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 UniProt Comments for YWHAG    14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; cytosol

Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; insulin-like growth factor receptor binding; protein binding; protein kinase C binding; receptor tyrosine kinase binding

Biological Process: cellular response to insulin stimulus; apoptosis; regulation of neuron differentiation; organelle organization and biogenesis; regulation of signal transduction; negative regulation of protein kinase activity; regulation of synaptic plasticity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein targeting
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 Research Articles on YWHAG    1. The normalisation capability of 14-3-3 gamma was superior to traditional LC in quantifying Western blot signals of the platelet AD-biomarker Monoamine Oxidase B of patient versus controls.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with YWHAG elisa kitDiseases associated with YWHAG elisa kit
 Products by Pathway  Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Centrosome Maturation Pathway antibodies  Centrosome Maturation Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with YWHAG and Nervous System Diseases
 Brain Diseases Antibodies  >3 publications with YWHAG and Brain Diseases
 Heart Diseases Antibodies  >2 publications with YWHAG and Heart Diseases
 Congenital Abnormalities Antibodies  >2 publications with YWHAG and Congenital Abnormalities
 Learning Disorders Antibodies  >1 publications with YWHAG and Learning Disorders
 Disease Models, Animal Antibodies  >1 publications with YWHAG and Disease Models, Animal
 Hypertrophy Antibodies  >1 publications with YWHAG and Hypertrophy
 Necrosis Antibodies  >1 publications with YWHAG and Necrosis
Organs/Tissues associated with YWHAG elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with YWHAG and Brain
 Heart Antibodies  >3 publications with YWHAG and Heart
 Muscle Antibodies  >3 publications with YWHAG and Muscle
 Nerve Antibodies  >3 publications with YWHAG and Nerve
 Bone Antibodies  >2 publications with YWHAG and Bone
 Bone Marrow Antibodies  >2 publications with YWHAG and Bone Marrow
 Blood Antibodies  >1 publications with YWHAG and Blood
 Lung Antibodies  >1 publications with YWHAG and Lung
 Kidney Antibodies  >1 publications with YWHAG and Kidney
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