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HSD17B13 elisa kit :: Mouse 17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7251954
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13), ELISA Kit

★Popular Item★
 Also Known As   

Mouse 17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13) ELISA Kit

 Product Gene Name   

HSD17B13 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    612127
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of HSD17B13. No significant cross-reactivity or interference between HSD17B13 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSD17B13 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This HSD17B13 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HSD17B13. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HSD17B13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HSD17B13 purchase    MBS7251954 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing HSD17B13, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7251954 kit is based on HSD17B13 antibody-HSD17B13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSD17B13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSD17B13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HSD17B13 elisa kit

   Principle of the assay: HSD17B13 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSD17B13 antibody and an HSD17B13-HRP conjugate. The assay sample and buffer are incubated together with HSD17B13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSD17B13 concentration since HSD17B13 from samples and HSD17B13-HRP conjugate compete for the anti-HSD17B13 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSD17B13 from the sample, fewer sites are left to bind HSD17B13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSD17B13 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for HSD17B13 elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of HSD17B13 elisa kit    HSD17B13 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7251954. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HSD17B13. It may not necessarily be applicable to this product.
 NCBI GI #    210032110
 NCBI GeneID    345275
 NCBI Accession #    NP_835236.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_178135.4 [Other Products]
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 UniProt Secondary Accession #    Q2M1L5; Q86W22; Q86W23; A8K9R9 [Other Products]
 UniProt Related Accession #    Q7Z5P4 [Other Products]
 Molecular Weight    29,605 Da [Similar Products]
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 NCBI Official Full Name    17-beta-hydroxysteroid dehydrogenase 13 isoform A
 NCBI Official Synonym Full Names    hydroxysteroid (17-beta) dehydrogenase 13
 NCBI Official Symbol    HSD17B13 [Similar Products]
 NCBI Official Synonym Symbols   
SCDR9; NIIL497; SDR16C3; HMFN0376
[Similar Products]
 NCBI Protein Information    17-beta-hydroxysteroid dehydrogenase 13; 17-beta-HSD 13; 17-beta hydroxysteroid dehydrogenase; short-chain dehydrogenase/reductase 9; short chain dehydrogenase/reductase family 16C, member 3
 UniProt Protein Name    17-beta-hydroxysteroid dehydrogenase 13
 UniProt Synonym Protein Names   
Short-chain dehydrogenase/reductase 9
 Protein Family    17-beta-hydroxysteroid dehydrogenase
 UniProt Gene Name    HSD17B13 [Similar Products]
 UniProt Synonym Gene Names    SCDR9; HMFN0376; UNQ497/PRO1014; 17-beta-HSD 13 [Similar Products]
 UniProt Entry Name    DHB13_HUMAN
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 UniProt Comments for HSD17B13    HSD17B13: Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Oxidoreductase; Secreted; EC 1.1.-.-

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: extracellular region; lipid particle

Molecular Function: oxidoreductase activity

Biological Process: positive regulation of lipid biosynthetic process
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 Research Articles on HSD17B13    1. A new human short-chain dehydrogenase/reductase gene SCDR9 is cloned and expressed.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with HSD17B13 elisa kitOrgans/Tissues associated with HSD17B13 elisa kit
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >1 publications with HSD17B13 and Fatty Liver
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2 publications with HSD17B13 and Liver
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