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Hsd3b1 elisa kit :: Mouse 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2887667
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (Hsd3b1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit

 Product Synonym Names    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; Hsd3b1; Hsd3b
 Product Gene Name   

Hsd3b1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2887667 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P24815
 Species Reactivity    Mouse
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Hsd3b1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Hsd3b1 purchase    MBS2887667 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (Hsd3b1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hsd3b1. The ELISA analytical biochemical technique of the MBS2887667 kit is based on Hsd3b1 antibody-Hsd3b1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hsd3b1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Hsd3b1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Hsd3b1 elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Hsd3b1 elisa kit    Hsd3b1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2887667. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Hsd3b1. It may not necessarily be applicable to this product.
 NCBI GI #    756398329
 NCBI GeneID    15492
 NCBI Accession #    NP_001291729.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001304800.1 [Other Products]
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 UniProt Primary Accession #    P24815 [Other Products]
 UniProt Secondary Accession #    Q7TQ00 [Other Products]
 UniProt Related Accession #    P24815 [Other Products]
 Molecular Weight    42,062 Da
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 NCBI Official Full Name    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
 NCBI Official Synonym Full Names    hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
 NCBI Official Symbol    Hsd3b1 [Similar Products]
 NCBI Official Synonym Symbols   
D3Ertd383e; 3-beta-HSD I
[Similar Products]
 NCBI Protein Information    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
 UniProt Protein Name    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
 UniProt Synonym Protein Names   
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I
 Protein Family    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
 UniProt Gene Name    Hsd3b1 [Similar Products]
 UniProt Synonym Gene Names    Hsd3b; 3-beta-HSD I [Similar Products]
 UniProt Entry Name    3BHS1_MOUSE
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 UniProt Comments for Hsd3b1    HSD3B1: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17- alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone. Belongs to the 3-beta-HSD family.

Protein type: EC 1.1.1.145; EC 5.3.3.1; Isomerase; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Mitochondrial; Oxidoreductase

Cellular Component: mitochondrion

Molecular Function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity; steroid delta-isomerase activity

Biological Process: steroid biosynthetic process
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 Research Articles on Hsd3b1    1. LH induction of 3beta-HSD in nonpregnant mouse endometrium.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Hsd3b1 elisa kitPathways associated with Hsd3b1 elisa kit
 Reference Product  PubMed Publications
 Cyp11a1 elisa kit  >75 publications with Hsd3b1 and Cyp11a1
 Cyp17a1 elisa kit  >61 publications with Hsd3b1 and Cyp17a1
 Cyp19a1 elisa kit  >52 publications with Hsd3b1 and Cyp19a1
 Srd5a1 elisa kit  >19 publications with Hsd3b1 and Srd5a1
 Hsd17b3 elisa kit  >17 publications with Hsd3b1 and Hsd17b3
 Cyp11b2 elisa kit  >9 publications with Hsd3b1 and Cyp11b2
 Cyp11b1 elisa kit  >7 publications with Hsd3b1 and Cyp11b1
 Cyp21a1 elisa kit  >3 publications with Hsd3b1 and Cyp21a1
 Products by Pathway  Pathway Diagram
 Aldosterone Synthesis And Secretion Pathway antibodies  Aldosterone Synthesis And Secretion Pathway Diagram
 Aldosterone Synthesis And Secretion Pathway antibodies  Aldosterone Synthesis And Secretion Pathway Diagram
 Androgen Biosynthesis Pathway antibodies  Androgen Biosynthesis Pathway Diagram
 C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies  C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
 C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies  C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
 Glucocorticoid & Mineralcorticoid Metabolism Pathway antibodies  Glucocorticoid & Mineralcorticoid Metabolism Pathway Diagram
 Glucocorticoid Biosynthesis Pathway antibodies  Glucocorticoid Biosynthesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
Diseases associated with Hsd3b1 elisa kitOrgans/Tissues associated with Hsd3b1 elisa kit
 Disease Name  Pubmed Publications
 Genital Diseases, Male Antibodies  >18 publications with Hsd3b1 and Genital Diseases, Male
 Hyperplasia Antibodies  >15 publications with Hsd3b1 and Hyperplasia
 Hypertension Antibodies  >12 publications with Hsd3b1 and Hypertension
 Prostatic Neoplasms Antibodies  >12 publications with Hsd3b1 and Prostatic Neoplasms
 Cardiovascular Diseases Antibodies  >11 publications with Hsd3b1 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >7 publications with Hsd3b1 and Breast Neoplasms
 Necrosis Antibodies  >5 publications with Hsd3b1 and Necrosis
 Obesity Antibodies  >3 publications with Hsd3b1 and Obesity
 Hypertrophy Antibodies  >3 publications with Hsd3b1 and Hypertrophy
 Infertility, Male Antibodies  >3 publications with Hsd3b1 and Infertility, Male
 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >45 publications with Hsd3b1 and Ovary
 Other Antibodies  >42 publications with Hsd3b1 and Other
 Testis Antibodies  >42 publications with Hsd3b1 and Testis
 Blood Antibodies  >36 publications with Hsd3b1 and Blood
 Adrenal Gland Antibodies  >17 publications with Hsd3b1 and Adrenal Gland
 Embryonic Tissue Antibodies  >11 publications with Hsd3b1 and Embryonic Tissue
 Uterus Antibodies  >8 publications with Hsd3b1 and Uterus
 Liver Antibodies  >6 publications with Hsd3b1 and Liver
 Kidney Antibodies  >4 publications with Hsd3b1 and Kidney
 Extraembryonic Tissue Antibodies  >2 publications with Hsd3b1 and Extraembryonic Tissue
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