Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow 5-LOX rightarrow Mouse 5-LOX  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • RANBP1 Antibody
 • BCKDK Antibody
 • Surfactant Associated Protein A (SP-A) ELISA Kit
 • Collagen alpha-1 (X) chain (Col10a1) Recombinant Protein
 • Soluble Cluster of differentiation 86 (sCD86) ELISA Kit
 • B9D1 Antibody
 • Leuconostoc mesenteroides subsp. Cremoris D-lactate dehydrog... Recombinant Protein
 • PFKL Antibody
 • Helicobacter pylori Vacuolating cytotoxin autotransporter (H... Antibody
 • Ataxin 2 (ATXN2) Antibody
 • P 38 Mitogen-Activated Protein Kinase (P38MAPK) ELISA Kit
 • BLOC1S6 Antibody
 • PSAP ELISA Kit
 • Permeability Glycoprotein (Pgp) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

5-LOX elisa kit :: Mouse 5-lipoxygenase (5-LOX) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2601248
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

5-lipoxygenase (5-LOX), ELISA Kit

★Popular Item★
 Also Known As   

Mouse 5-lipoxygenase (5-LOX) ELISA Kit

 Product Gene Name   

5-LOX elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    152390
Section Bar
 Species Reactivity    Mouse
Section Bar
 Specificity    No cross-reaction with other factors.
Section Bar
 Samples    Serum, plasma or cell culture supernatant and organizations in the natural and recombinant 5-LOX concentration
 Assay Type    Sandwich
 Detection Range    10 ng/ml-0.156 ng/ml
 Sensitivity    0.05 ng/ml.
 Intra-assay Precision    ? 8%
 Inter-assay Precision    ? 12%
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of 5-LOX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for 5-LOX purchase    MBS2601248 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-lipoxygenase (5-LOX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156 ng/ml in biological research samples containing 5-LOX, with an estimated sensitivity of 0.05 ng/ml. The ELISA analytical biochemical technique of the MBS2601248 kit is based on 5-LOX antibody-5-LOX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 5-LOX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 5-LOX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant and organizations in the natural and recombinant 5-LOX concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for 5-LOX elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse 5-LOX monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of 5-LOX elisa kit    5-LOX elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS2601248. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for 5-LOX. It may not necessarily be applicable to this product.
 NCBI GI #    187167
 NCBI GeneID    240
 NCBI Accession #    AAA63212.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q5JQ14; B7ZLS0; E5FPY5; E5FPY7; E5FPY8 [Other Products]
 UniProt Related Accession #    P09917 [Other Products]
 Molecular Weight    56,291 Da
Table BarTOPTable Bar
 NCBI Official Full Name    5-lipoxygenase, partial
 NCBI Official Synonym Full Names    arachidonate 5-lipoxygenase
 NCBI Official Symbol    ALOX5 [Similar Products]
 NCBI Official Synonym Symbols   
5-LO; 5LPG; LOG5; 5-LOX
[Similar Products]
 NCBI Protein Information    arachidonate 5-lipoxygenase; LOX-5; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform
 UniProt Protein Name    Arachidonate 5-lipoxygenase
 UniProt Gene Name    ALOX5 [Similar Products]
 UniProt Synonym Gene Names    LOG5; 5-LO; 5-lipoxygenase [Similar Products]
 UniProt Entry Name    LOX5_HUMAN
Section Bar
 NCBI Summary for 5-LOX    This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Section Bar
 UniProt Comments for 5-LOX    5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.

Protein type: EC 1.13.11.34; Lipid Metabolism - arachidonic acid; Oxidoreductase; Nuclear envelope; Lipid Metabolism - linoleic acid

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol

Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity

Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Asthma, Susceptibility To
Table BarTOPTable Bar
 Research Articles on 5-LOX    1. Polymorphisms within FLT3, EGFR, NEIL3, and ALOX5 may contribute to the occurrence of GBM.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with 5-LOX elisa kitDiseases associated with 5-LOX elisa kit
 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Eicosanoid Synthesis Pathway antibodies  Eicosanoid Synthesis Pathway Diagram
 IL-5 Signaling Pathway antibodies  IL-5 Signaling Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Ovarian Steroidogenesis Pathway antibodies  Ovarian Steroidogenesis Pathway Diagram
 Ovarian Steroidogenesis Pathway antibodies  Ovarian Steroidogenesis Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >586 publications with 5-LOX and Inflammation
 Asthma Antibodies  >251 publications with 5-LOX and Asthma
 Lung Diseases Antibodies  >237 publications with 5-LOX and Lung Diseases
 Atherosclerosis Antibodies  >123 publications with 5-LOX and Atherosclerosis
 Necrosis Antibodies  >115 publications with 5-LOX and Necrosis
 Edema Antibodies  >94 publications with 5-LOX and Edema
 Heart Diseases Antibodies  >59 publications with 5-LOX and Heart Diseases
 Neoplasms, Experimental Antibodies  >52 publications with 5-LOX and Neoplasms, Experimental
 Pain Antibodies  >49 publications with 5-LOX and Pain
 Reperfusion Injury Antibodies  >43 publications with 5-LOX and Reperfusion Injury
Organs/Tissues associated with 5-LOX elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1141 publications with 5-LOX and Blood
 Lung Antibodies  >258 publications with 5-LOX and Lung
 Brain Antibodies  >134 publications with 5-LOX and Brain
 Muscle Antibodies  >118 publications with 5-LOX and Muscle
 Bone Antibodies  >81 publications with 5-LOX and Bone
 Heart Antibodies  >72 publications with 5-LOX and Heart
 Kidney Antibodies  >66 publications with 5-LOX and Kidney
 Bone Marrow Antibodies  >59 publications with 5-LOX and Bone Marrow
 Intestine Antibodies  >51 publications with 5-LOX and Intestine
 Prostate Antibodies  >39 publications with 5-LOX and Prostate
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions