Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow CH50 rightarrow Mouse CH50  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • kielin-chordin-like protein (KCP) ELISA Kit
 • G protein-coupled receptor 120 (GPR120) ELISA Kit
 • diaphanous homolog 1 (EHI_118260) Antibody
 • RAC1-S71 Antibody
 • Calcium Sensing Receptor (CASR) Antibody
 • N-Terminal Brain Natriuretic Peptide (NT-BNP) ELISA Kit
 • Microcystin-LR Antibody
 • S100 calcium binding protein A2 (S100A2) ELISA Kit
 • RSV Antibody
 • TGF beta1 Antibody
 • Sulfotransferase 1A3-1A4 (SULT1A3) Recombinant Protein
 • microtubule-associated protein 1 light chain 3 beta (MAP1LC3... ELISA Kit
 • Myoglobin (MB) Recombinant Protein
 • Chlamydia pneumoniae antibody (Cpn-IgG) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CH50 elisa kit :: Mouse 50% complement hemolysis (CH50) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS264677
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $290 +1 FREE 8GB USB
96-Strip-Wells  /  $450 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

50% complement hemolysis (CH50), ELISA Kit

★Popular Item★
 Also Known As   

Mouse 50% complement hemolysis (CH50) ELISA Kit

 Product Gene Name   

CH50 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Mouse
Section Bar
 Specificity    No cross-reaction with other factors.
Section Bar
 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    100 U/ml-1.56 U/ml
 Sensitivity    Up to 0.5 U/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CH50 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CH50 purchase    MBS264677 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 50% complement hemolysis (CH50) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 100 U/ml-1.56 U/ml in biological research samples containing CH50, with an estimated sensitivity of Up to 0.5 U/ml. The ELISA analytical biochemical technique of the MBS264677 kit is based on CH50 antibody-CH50 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CH50 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CH50. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for CH50 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse CH50 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
Section Bar
 Typical Standard Curve/Testing Data of CH50 elisa kit    CH50 elisa kit Typical Standard Curve/Testing Data image
Table BarTOPTable Bar
Sample Manual Insert of MBS264677. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CH50. It may not necessarily be applicable to this product.
 NCBI GI #    262050656
 NCBI GeneID    280678
 NCBI Accession #    NP_001159957.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001166485.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q27992; Q27993 [Other Products]
 UniProt Related Accession #    P01030 [Other Products]
 Molecular Weight    101,885 Da
Table BarTOPTable Bar
 NCBI Official Full Name    complement C4
 NCBI Official Symbol    C4A [Similar Products]
 NCBI Official Synonym Symbols   
C4
[Similar Products]
 NCBI Protein Information    complement C4
 UniProt Protein Name    Complement C4
 UniProt Gene Name    C4 [Similar Products]
 UniProt Entry Name    CO4_BOVIN
Section Bar
 UniProt Comments for CH50    Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes ().
Table BarTOPTable Bar
 Research Articles on CH50    1. The C4A-complete transcript is the main transcript under normal physiological conditions, while C4A-AS is augmented when mastitis develops.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with CH50 elisa kitPathways associated with CH50 elisa kit
 Reference Product  PubMed Publications
 C1S elisa kit  >28 publications with CH50 and C1S
 C8A elisa kit  >4 publications with CH50 and C8A
 C4BPA elisa kit  >1 publications with CH50 and C4BPA
 C1QB elisa kit  >1 publications with CH50 and C1QB
 MASP2 elisa kit  >1 publications with CH50 and MASP2
 Products by Pathway  Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram
Organs/Tissues associated with CH50 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >66 publications with CH50 and Liver
 Kidney Antibodies  >52 publications with CH50 and Kidney
 Skin Antibodies  >39 publications with CH50 and Skin
 Brain Antibodies  >18 publications with CH50 and Brain
 Muscle Antibodies  >18 publications with CH50 and Muscle
 Whole Body Antibodies  >3 publications with CH50 and Whole Body
 Intestine Antibodies  >3 publications with CH50 and Intestine
 Mammary Gland Antibodies  >2 publications with CH50 and Mammary Gland
 Adipose Tissue Antibodies  >2 publications with CH50 and Adipose Tissue
 Ovary Antibodies  >2 publications with CH50 and Ovary
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions