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| Product Name
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A Disintegrin And Metalloprotease (ADAM), ELISA Kit ★Popular Item★
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| Also Known As
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Mouse A Disintegrin And Metalloprotease ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| Species Reactivity
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Mouse
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| Samples
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Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
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| Assay Type
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Sandwich
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| Detection Range
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1.0-25ng/mL
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| Sensitivity
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0.1ng/mL
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| Intended Uses
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This ADAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ADAM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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| Preparation and Storage
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Store all reagents at 2-8 degree C.
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| Sample Preparation
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We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of ADAM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for ADAM purchase
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MBS740613 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloprotease (ADAM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing ADAM, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS740613 kit is based on ADAM antibody-ADAM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADAM elisa kit
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Principle of the Assay: ADAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM antibody and an ADAM-HRP conjugate. The assay sample and buffer are incubated together with ADAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM concentration since ADAM from samples and ADAM-HRP conjugate compete for the anti-ADAM antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM from the sample, fewer sites are left to bind ADAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM concentration in each sample is interpolated from this standard curve.
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| Product Categories/Family for ADAM elisa kit
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Cardiovascular
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| Typical Testing Data/Standard Curve (for reference only) of ADAM elisa kit
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| Sample Manual Insert of MBS740613. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for ADAM. It may not necessarily be applicable to this product. |
| NCBI GI #
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133778949
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| NCBI GeneID
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108153
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| NCBI Accession #
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NP_001003911.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_001003911.2
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[Other Products]
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| UniProt Secondary Accession #
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B2RUI8; E9QMY0
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[Other Products]
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| UniProt Related Accession #
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Q68SA9
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[Other Products]
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| Molecular Weight
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177,712 Da
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| NCBI Official Full Name
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A disintegrin and metalloproteinase with thrombospondin motifs 7
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| NCBI Official Synonym Full Names
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a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 7
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| NCBI Official Symbol
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Adamts7
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[Similar Products]
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| NCBI Official Synonym Symbols
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ADAM-TS7; ADAMTS7B
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[Similar Products]
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| NCBI Protein Information
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A disintegrin and metalloproteinase with thrombospondin motifs 7; COMPase; ADAMTS-7; ADAM-TS 7; metalloprotease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 7
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| UniProt Protein Name
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A disintegrin and metalloproteinase with thrombospondin motifs 7
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| UniProt Synonym Protein Names
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COMPase
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| Protein Family
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Eukaryotic translation initiation factor
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| UniProt Gene Name
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Adamts7
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[Similar Products]
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| UniProt Synonym Gene Names
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ADAM-TS 7; ADAM-TS7; ADAMTS-7
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[Similar Products]
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| UniProt Entry Name
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ATS7_MOUSE
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| UniProt Comments for ADAM
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ADAMTS7: Metalloprotease that may play a role in the degradation of COMP.
Protein type: Motility/polarity/chemotaxis; Cell surface; EC 3.4.24.-; Secreted; Protease; Secreted, signal peptide
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; cell surface; extracellular region
Molecular Function: peptidase activity; zinc ion binding; metallopeptidase activity; hydrolase activity; metalloendopeptidase activity; metal ion binding
Biological Process: proteolysis involved in cellular protein catabolic process; negative regulation of chondrocyte differentiation; proteolysis
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| Research Articles on ADAM
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1. ADAMTS-7 and TNF-alpha form a positive feedback loop in the regulation of cartilage degradation and osteoarthritis progression.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Pathways associated with ADAM elisa kit | Diseases associated with ADAM elisa kit |
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| Organs/Tissues associated with ADAM elisa kit |
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ELISA Kit 
