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AHSA1 elisa kit :: Mouse AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS906159
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) (AHSA1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Activator of 90 kDa heat shock protein ATPase homolog 1, AHSA1 ELISA Kit

 Product Synonym Names    Mouse Activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1) ELISA kit; AHA1; C14orf3; p38; activator of heat shock 90kDa protein ATPase homolog 1; AHA1; activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
 Product Gene Name   

AHSA1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS906159 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q8BK64
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse AHSA1. No significant cross-reactivity or interference between Mouse AHSA1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    28 pg/ml-1800 pg/ml
 Sensitivity    7 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Neurobiology
 Protein Biological Process 3    Stress response
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AHSA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AHSA1 purchase    MBS906159 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) (AHSA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 28 pg/ml-1800 pg/ml in biological research samples containing AHSA1, with an estimated sensitivity of 7 pg/ml. The ELISA analytical biochemical technique of the MBS906159 kit is based on AHSA1 antibody-AHSA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AHSA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AHSA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AHSA1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AHSA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AHSA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AHSA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AHSA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of AHSA1 elisa kit    AHSA1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS906159. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AHSA1. It may not necessarily be applicable to this product.
 NCBI GI #    22122515
 NCBI GeneID    217737
 NCBI Accession #    NP_666148.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_146036.1 [Other Products]
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 UniProt Primary Accession #    Q8BK64 [Other Products]
 UniProt Secondary Accession #    Q8R3E6 [Other Products]
 UniProt Related Accession #    Q8BK64 [Other Products]
 Molecular Weight    38,117 Da
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 NCBI Official Full Name    activator of 90 kDa heat shock protein ATPase homolog 1
 NCBI Official Synonym Full Names    AHA1, activator of heat shock protein ATPase 1
 NCBI Official Symbol    Ahsa1 [Similar Products]
 NCBI Official Synonym Symbols   
p38; BC023857
[Similar Products]
 NCBI Protein Information    activator of 90 kDa heat shock protein ATPase homolog 1; AHA1, activator of heat shock protein ATPase homolog 1; AHA1, activator of heat shock 90kDa protein ATPase homolog 1
 UniProt Protein Name    Activator of 90 kDa heat shock protein ATPase homolog 1
 Protein Family    Activator of 90 kDa heat shock protein ATPase
 UniProt Gene Name    Ahsa1 [Similar Products]
 UniProt Synonym Gene Names    AHA1 [Similar Products]
 UniProt Entry Name    AHSA1_MOUSE
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 UniProt Comments for AHSA1    AHSA1: Cochaperone that stimulates HSP90 ATPase activity. May affect a step in the endoplasmic reticulum to Golgi trafficking. Belongs to the AHA1 family.

Protein type: Chaperone

Cellular Component: endoplasmic reticulum; cytoplasm

Molecular Function: ATPase activator activity; chaperone binding

Biological Process: positive regulation of ATPase activity; response to stress
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 Research Articles on AHSA1    1. Hsp90 phosphorylation on tyrosine313 promotes recruitment of AHA1, which stimulates Hsp90 ATPase activity, furthering the chaperoning process.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with AHSA1 elisa kitOrgans/Tissues associated with AHSA1 elisa kit
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >3 publications with AHSA1 and Fibrosis
 Neoplasms Antibodies  >1 publications with AHSA1 and Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with AHSA1 and Muscle
 Kidney Antibodies  >1 publications with AHSA1 and Kidney
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