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AIF elisa kit :: Mouse AIF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2503405
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AIF, ELISA Kit

★Popular Item★
 Also Known As   

Mouse AIF (Apoptosis Inducing Factor) ELISA Kit

 Product Gene Name   

AIF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    300169
 3D Structure    ModBase 3D Structure for O95831
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse AIF. No significant cross-reactivity or interference between Mouse AIF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse AIF and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    7.813-500pg/mL
 Sensitivity    Min: 4.688pg/mL; Max: 500pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AIF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AIF purchase    MBS2503405 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AIF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.813-500pg/mL in biological research samples containing AIF, with an estimated sensitivity of Min: 4.688pg/mL; Max: 500pg/mL. The ELISA analytical biochemical technique of the MBS2503405 kit is based on AIF antibody-AIF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AIF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AIF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AIF elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AIF in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AIF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse AIF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse AIF. You can calculate the concentration of Mouse AIF in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of AIF elisa kit    AIF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2503405. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AIF. It may not necessarily be applicable to this product.
 NCBI GI #    4757732
 NCBI GeneID    9131
 NCBI Accession #    NP_004199.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004208.3 [Other Products]
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 UniProt Primary Accession #    O95831 [Other Products]
 UniProt Secondary Accession #    Q1L6K4; Q1L6K6; Q2QKE4; Q5JUZ7; Q6I9X6; Q9Y3I3; Q9Y3I4; A4QPB4; B1ALN1; B2RB08; D3DTE9 [Other Products]
 UniProt Related Accession #    O95831 [Other Products]
 Molecular Weight    26,033 Da
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 NCBI Official Full Name    apoptosis-inducing factor 1, mitochondrial isoform 1
 NCBI Official Synonym Full Names    apoptosis-inducing factor, mitochondrion-associated, 1
 NCBI Official Symbol    AIFM1 [Similar Products]
 NCBI Official Synonym Symbols   
AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6
[Similar Products]
 NCBI Protein Information    apoptosis-inducing factor 1, mitochondrial; Neuropathy, axonal motor-sensory, with deafness and mental retardation; neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome); programmed cell death 8 (apoptosis-inducing factor); striatal apoptosis-inducing factor
 UniProt Protein Name    Apoptosis-inducing factor 1, mitochondrial
 UniProt Synonym Protein Names   
Programmed cell death protein 8
 Protein Family    Putative apoptosis-inducing factor
 UniProt Gene Name    AIFM1 [Similar Products]
 UniProt Synonym Gene Names    AIF; PDCD8 [Similar Products]
 UniProt Entry Name    AIFM1_HUMAN
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 NCBI Summary for AIF    This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]
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 UniProt Comments for AIF    PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Apoptosis; EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; oxidoreductase activity, acting on NADH or NADPH; electron carrier activity; DNA binding; NAD(P)H oxidase activity

Biological Process: chromosome condensation; caspase activation; neuron differentiation; mitochondrial respiratory chain complex I assembly; neuron apoptosis; cell redox homeostasis; apoptosis; positive regulation of apoptosis; DNA fragmentation during apoptosis; DNA catabolic process

Disease: Cowchock Syndrome
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 Research Articles on AIF    1. review describes the multistep course of parthanatos and its dependence on PAR signalling and nuclear AIF translocation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AIF elisa kitPathways associated with AIF elisa kit
 Reference Product  PubMed Publications
 CASP3 elisa kit  >101 publications with AIF and CASP3
 XIAP elisa kit  >12 publications with AIF and XIAP
 ENDOG elisa kit  >5 publications with AIF and ENDOG
 HSP72 elisa kit  >1 publications with AIF and HSP72
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation By HSP70 Pathway antibodies  Apoptosis Modulation By HSP70 Pathway Diagram
 Ceramide Signaling Pathway antibodies  Ceramide Signaling Pathway Diagram
Diseases associated with AIF elisa kitOrgans/Tissues associated with AIF elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >36 publications with AIF and Necrosis
 Nervous System Diseases Antibodies  >28 publications with AIF and Nervous System Diseases
 Brain Diseases Antibodies  >25 publications with AIF and Brain Diseases
 Breast Neoplasms Antibodies  >16 publications with AIF and Breast Neoplasms
 Carcinoma, Hepatocellular Antibodies  >11 publications with AIF and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >10 publications with AIF and Liver Neoplasms
 Neoplasms, Experimental Antibodies  >4 publications with AIF and Neoplasms, Experimental
 Heart Diseases Antibodies  >4 publications with AIF and Heart Diseases
 Disease Models, Animal Antibodies  >4 publications with AIF and Disease Models, Animal
 Inflammation Antibodies  >3 publications with AIF and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >33 publications with AIF and Blood
 Brain Antibodies  >33 publications with AIF and Brain
 Lung Antibodies  >19 publications with AIF and Lung
 Liver Antibodies  >15 publications with AIF and Liver
 Muscle Antibodies  >13 publications with AIF and Muscle
 Vascular Antibodies  >9 publications with AIF and Vascular
 Heart Antibodies  >8 publications with AIF and Heart
 Prostate Antibodies  >8 publications with AIF and Prostate
 Skin Antibodies  >8 publications with AIF and Skin
 Stomach Antibodies  >6 publications with AIF and Stomach
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