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ANGPTL3 elisa kit :: Mouse ANGPTL3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS177160
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ANGPTL3, ELISA Kit

★Popular Item★
 Also Known As   

Mouse ANGPTL3 PicoKine ELISA Kit

 Product Synonym Names    Angiopoietin-related protein 3; angiopoietin-like 3; ANG 5; ANG-5; ANG5; Angiopoietin 5; Angiopoietin like 3; Angiopoietin related protein 3; Angiopoietin-5; Angiopoietin-like protein 3; Angiopoietin-related protein 3; ANGL3_HUMAN; ANGPT5; ANGPTL3; ANL3; FHBL2; OTTHUMP00000010719
 Product Gene Name   

ANGPTL3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9R182
 Species Reactivity    Mouse
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 Specificity    Natural and recombinant mouse ANGPTL3
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 Samples    Cell culture supernates, serum and plasma (heparin, EDTA)
 Assay Type    Sandwich
 Detection Range    156pg/ml-10,000pg/ml
 Sensitivity    < 10pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ANGPTL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ANGPTL3 purchase    MBS177160 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ANGPTL3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 156pg/ml-10,000pg/ml in biological research samples containing ANGPTL3, with an estimated sensitivity of < 10pg/ml. The ELISA analytical biochemical technique of the MBS177160 kit is based on ANGPTL3 antibody-ANGPTL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANGPTL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANGPTL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma (heparin, EDTA). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ANGPTL3 elisa kit

   Principle of the assay: mouse ANGPTL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ANGPTL3 has been precoated onto 96-well plates. Standards(sf21, S17-T455) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ANGPTL3 amount of sample captured in plate.
Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
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 Typical Testing Data/Standard Curve (for reference only) of ANGPTL3 elisa kit    Mouse ANGPTL3 PicoKine ELISA Kit standard curve
ANGPTL3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS177160. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ANGPTL3. It may not necessarily be applicable to this product.
 NCBI GI #    25008124
 NCBI GeneID    30924
 NCBI Accession #    Q9R182.1 [Other Products]
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 UniProt Primary Accession #    Q9R182 [Other Products]
 UniProt Related Accession #    Q9R182 [Other Products]
 Molecular Weight    52,543 Da
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 NCBI Official Full Name    Angiopoietin-related protein 3
 NCBI Official Synonym Full Names    angiopoietin-like 3
 NCBI Official Symbol    Angptl3 [Similar Products]
 NCBI Official Synonym Symbols   
hypl
[Similar Products]
 NCBI Protein Information    angiopoietin-related protein 3; hypolipidemia; angiopoietin-like protein 3
 UniProt Protein Name    Angiopoietin-related protein 3
 UniProt Synonym Protein Names   
Angiopoietin-like protein 3
 Protein Family    Angiopoietin-related protein
 UniProt Gene Name    Angptl3 [Similar Products]
 UniProt Entry Name    ANGL3_MOUSE
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 NCBI Summary for ANGPTL3    This gene encodes a member of the angiopoietin-like family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may inhibit triglyceride metabolism. Homozygous knockout mice for this gene exhibit reduced plasma lipid concentrations, including reduced plasma triglyceride concentrations, and enhanced activity of enzymes involved in triglyceride metabolism. [provided by RefSeq, Aug 2015]
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 UniProt Comments for ANGPTL3    ANGPTL3: Defects in ANGPTL3 are the cause of familial hypobetalipoproteinemia type 2 (FHBL2); also called combined hypobetalipoproteinemia familial. FHBL2 is a disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Inhibitor

Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome; extracellular region

Molecular Function: integrin binding; enzyme inhibitor activity; phospholipase inhibitor activity; growth factor activity

Biological Process: cholesterol metabolic process; phospholipid catabolic process; glycerol metabolic process; cell-matrix adhesion; lipid homeostasis; positive regulation of lipid catabolic process; fatty acid metabolic process; signal transduction; positive regulation of angiogenesis; sequestering of lipid; cholesterol homeostasis; triacylglycerol metabolic process; acylglycerol homeostasis; phospholipid metabolic process; negative regulation of catalytic activity; artery morphogenesis; positive regulation of lipid metabolic process; negative regulation of lipoprotein lipase activity; positive regulation of cell migration; phospholipid homeostasis
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 Research Articles on ANGPTL3    1. Angptl3 could induce actin filament rearrangement, mainly in lamellipodia formation, and that this process was mediated by integrin alpha(V)beta-mediated FAK and PI3K phosphorylation and Rac1 activation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ANGPTL3 elisa kitDiseases associated with ANGPTL3 elisa kit
 Products by Pathway  Pathway Diagram
 PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies  PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
 XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies  XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >22 publications with ANGPTL3 and Cardiovascular Diseases
 Fatty Liver Antibodies  >19 publications with ANGPTL3 and Fatty Liver
 Liver Diseases Antibodies  >12 publications with ANGPTL3 and Liver Diseases
 Disease Models, Animal Antibodies  >6 publications with ANGPTL3 and Disease Models, Animal
 Carcinoma, Hepatocellular Antibodies  >4 publications with ANGPTL3 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >3 publications with ANGPTL3 and Liver Neoplasms
 Kidney Diseases Antibodies  >3 publications with ANGPTL3 and Kidney Diseases
 Proteinuria Antibodies  >3 publications with ANGPTL3 and Proteinuria
 Inflammation Antibodies  >3 publications with ANGPTL3 and Inflammation
 Hypertension Antibodies  >2 publications with ANGPTL3 and Hypertension
Organs/Tissues associated with ANGPTL3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >63 publications with ANGPTL3 and Liver
 Heart Antibodies  >19 publications with ANGPTL3 and Heart
 Kidney Antibodies  >7 publications with ANGPTL3 and Kidney
 Brain Antibodies  >4 publications with ANGPTL3 and Brain
 Lung Antibodies  >3 publications with ANGPTL3 and Lung
 Eye Antibodies  >1 publications with ANGPTL3 and Eye
 Embryonic Tissue Antibodies  >1 publications with ANGPTL3 and Embryonic Tissue
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