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APO-E elisa kit :: Mouse APO-E ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2512274 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $310 +1 FREE 8GB USB
96-Strip-Wells  /  $355 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

APO-E, ELISA Kit

★Popular Item★
 Also Known As   

Mouse APO-E (Apolipoprotein E) ELISA Kit

 Product Gene Name   

APO-E elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2512274 COA    COA PDF
 OMIM    104310
 3D Structure    ModBase 3D Structure for P02649
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 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and some recombinant Mouse APO-E. No significant crossreactivity or interference between Mouse APO-E and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    1.56-100 ng/mL
 Sensitivity    Mouse APO-E is 0.94 ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of APO-E elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for APO-E purchase    MBS2512274 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the APO-E, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56-100 ng/mL in biological research samples containing APO-E, with an estimated sensitivity of Mouse APO-E is 0.94 ng/mL. The ELISA analytical biochemical technique of the MBS2512274 kit is based on APO-E antibody-APO-E antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APO-E antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APO-E. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for APO-E elisa kit

   Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Mouse APO-E concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Mouse APO-E. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Mouse APO-E and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Mouse APO-E, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse APO-E. The concentration of Mouse APO-E in samples can be calculated by comparing the OD of the samples with the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of APO-E elisa kit    APO-E elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2512274. Click to request current manual
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NCBI/Uniprot data below describe general gene information for APO-E. It may not necessarily be applicable to this product.
 NCBI GI #    4557325
 NCBI GeneID    348
 NCBI Accession #    NP_000032.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000041.3 [Other Products]
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 UniProt Primary Accession #    P02649 [Other Products]
 UniProt Secondary Accession #    Q9P2S4; B2RC15; C0JYY5 [Other Products]
 UniProt Related Accession #    P02649 [Other Products]
 Molecular Weight    36,154 Da
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 NCBI Official Full Name    apolipoprotein E isoform b
 NCBI Official Synonym Full Names    apolipoprotein E
 NCBI Official Symbol    APOE [Similar Products]
 NCBI Official Synonym Symbols   
AD2; LPG; APO-E; LDLCQ5
[Similar Products]
 NCBI Protein Information    apolipoprotein E; apolipoprotein E3
 UniProt Protein Name    Apolipoprotein E
 UniProt Gene Name    APOE [Similar Products]
 UniProt Synonym Gene Names    Apo-E [Similar Products]
 UniProt Entry Name    APOE_HUMAN
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 NCBI Summary for APO-E    The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
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 UniProt Comments for APO-E    APOE: Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. Defects in APOE are a cause of hyperlipoproteinemia type 3 (HLPP3); also known as familial dysbetalipoproteinemia. Individuals with HLPP3 are clinically characterized by xanthomas, yellowish lipid deposits in the palmar crease, or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women, it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE*2 alleles. More severe cases of HLPP3 have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE*4 variant are at higher risk of CAD. Genetic variations in APOE are associated with Alzheimer disease type 2 (AD2). It is a late-onset neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death. The APOE*4 allele is genetically associated with the common late onset familial and sporadic forms of Alzheimer disease. Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE*4 alleles in 42 families with late onset AD. Thus APOE*4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE*4 was virtually sufficient to cause AD by age 80. The mechanism by which APOE*4 participates in pathogenesis is not known. Defects in APOE are a cause of sea-blue histiocyte disease (SBHD); also known as sea-blue histiocytosis. This disorder is characterized by splenomegaly, mild thrombocytopenia and, in the bone marrow, numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses. Defects in APOE are a cause of lipoprotein glomerulopathy (LPG). LPG is an uncommon kidney disease characterized by proteinuria, progressive kidney failure, and distinctive lipoprotein thrombi in glomerular capillaries. It mainly affects people of Japanese and Chinese origin. The disorder has rarely been described in Caucasians. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted, signal peptide; Secreted; Lipid-binding

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: Golgi apparatus; microtubule; extracellular space; lysosome; endoplasmic reticulum; early endosome; dendrite; extracellular region; nuclear envelope; extracellular matrix; chylomicron; extrinsic to external side of plasma membrane; cell soma; membrane; late endosome; cytoplasm; plasma membrane; nucleus; vesicle

Molecular Function: heparin binding; lipid transporter activity; identical protein binding; protein homodimerization activity; metal chelating activity; beta-amyloid binding; cholesterol binding; antioxidant activity; protein binding; low-density lipoprotein receptor binding; hydroxyapatite binding; cholesterol transporter activity; phospholipid binding; lipid binding; tau protein binding

Biological Process: negative regulation of MAP kinase activity; lipoprotein catabolic process; phototransduction, visible light; cGMP-mediated signaling; positive regulation of axon extension; axon regeneration in the peripheral nervous system; positive regulation of membrane protein ectodomain proteolysis; synaptic transmission, cholinergic; intracellular transport; triacylglycerol catabolic process; oligodendrocyte differentiation; negative regulation of neuron apoptosis; cholesterol catabolic process; long-chain fatty acid transport; cholesterol metabolic process; regulation of Cdc42 protein signal transduction; positive regulation of nitric-oxide synthase activity; negative regulation of blood coagulation; lipoprotein metabolic process; positive regulation of lipid biosynthetic process; regulation of axon extension; negative regulation of blood vessel endothelial cell migration; maintenance of cellular localization; cholesterol homeostasis; response to reactive oxygen species; response to ethanol; positive regulation of cGMP biosynthetic process; regulation of gene expression; lipoprotein biosynthetic process; protein import; negative regulation of endothelial cell proliferation; nitric oxide mediated signal transduction; regulation of neuronal synaptic plasticity; response to dietary excess; vasodilation; response to insulin stimulus; positive regulation of low-density lipoprotein receptor catabolic process; phospholipid efflux; retinoid metabolic process; negative regulation of cholesterol biosynthetic process; aging; receptor-mediated endocytosis; response to retinoic acid; negative regulation of lipid biosynthetic process; neurite regeneration; cholesterol efflux; cytoskeleton organization and biogenesis; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; reverse cholesterol transport; triacylglycerol metabolic process; negative regulation of inflammatory response; fatty acid homeostasis; artery morphogenesis

Disease: Macular Degeneration, Age-related, 1; Alzheimer Disease 2; Alzheimer Disease 4; Lipoprotein Glomerulopathy; Sea-blue Histiocyte Disease
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 Research Articles on APO-E    1. Age and APOE 4 genotype were associated with higher cerebral uptake of the amyloid tracer 11C-PIB; in this sample of cognitively healthy elderly individuals, men exhibited higher 11C-PIB uptake than women
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with APO-E elisa kitPathways associated with APO-E elisa kit
 Reference Product  PubMed Publications
 APOB elisa kit  >1470 publications with APO-E and APOB
 LDLR elisa kit  >543 publications with APO-E and LDLR
 LPL elisa kit  >322 publications with APO-E and LPL
 SCARB1 elisa kit  >112 publications with APO-E and SCARB1
 MAPT elisa kit  >103 publications with APO-E and MAPT
 LRP1 elisa kit  >74 publications with APO-E and LRP1
 APOC3 elisa kit  >73 publications with APO-E and APOC3
 VLDLR elisa kit  >53 publications with APO-E and VLDLR
 LIPC elisa kit  >41 publications with APO-E and LIPC
 LRP8 elisa kit  >15 publications with APO-E and LRP8
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 HDL-mediated Lipid Transport Pathway antibodies  HDL-mediated Lipid Transport Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
Diseases associated with APO-E elisa kitOrgans/Tissues associated with APO-E elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >6008 publications with APO-E and Cardiovascular Diseases
 Alzheimer Disease Antibodies  >5597 publications with APO-E and Alzheimer Disease
 Atherosclerosis Antibodies  >4769 publications with APO-E and Atherosclerosis
 Arteriosclerosis Antibodies  >3968 publications with APO-E and Arteriosclerosis
 Hyperlipidemias Antibodies  >1803 publications with APO-E and Hyperlipidemias
 Inflammation Antibodies  >1584 publications with APO-E and Inflammation
 Cognition Disorders Antibodies  >1345 publications with APO-E and Cognition Disorders
 Plaque, Atherosclerotic Antibodies  >1205 publications with APO-E and Plaque, Atherosclerotic
 Coronary Disease Antibodies  >1122 publications with APO-E and Coronary Disease
 Hypercholesterolemia Antibodies  >1037 publications with APO-E and Hypercholesterolemia
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4307 publications with APO-E and Brain
 Vascular Antibodies  >4049 publications with APO-E and Vascular
 Liver Antibodies  >1861 publications with APO-E and Liver
 Heart Antibodies  >1568 publications with APO-E and Heart
 Muscle Antibodies  >1179 publications with APO-E and Muscle
 Nerve Antibodies  >624 publications with APO-E and Nerve
 Bone Antibodies  >575 publications with APO-E and Bone
 Kidney Antibodies  >502 publications with APO-E and Kidney
 Lung Antibodies  >269 publications with APO-E and Lung
 Spleen Antibodies  >232 publications with APO-E and Spleen
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