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ATP1b1 elisa kit :: Mouse ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022937 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1), ELISA Kit

 Also Known As   

ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) ELISA Kit

 Product Synonym Names    ATP1B; Sodium/potassium-dependent ATPase subunit beta-1
 Product Gene Name   

ATP1b1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    182330
 3D Structure    ModBase 3D Structure for P05026
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1).
No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.071ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ATP1b1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ATP1b1 purchase    MBS2022937 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing ATP1b1, with an estimated sensitivity of < 0.071ng/mL. The ELISA analytical biochemical technique of the MBS2022937 kit is based on ATP1b1 antibody-ATP1b1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP1b1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP1b1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ATP1b1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for ATP1b1 elisa kit    Signal transduction
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 Typical Testing Data/Standard Curve (for reference only) of ATP1b1 elisa kit    ATP1b1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022937. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ATP1b1. It may not necessarily be applicable to this product.
 NCBI GI #    119611239
 NCBI GeneID    481
 NCBI Accession #    EAW90833.1 [Other Products]
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 UniProt Primary Accession #    P05026 [Other Products]
 UniProt Secondary Accession #    Q5TGZ3 [Other Products]
 UniProt Related Accession #    P05026 [Other Products]
 Molecular Weight    34,893 Da
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 NCBI Official Full Name    ATPase, Na+/K+ transporting, beta 1 polypeptide, isoform CRA_b
 NCBI Official Synonym Full Names    ATPase, Na+/K+ transporting, beta 1 polypeptide
 NCBI Official Symbol    ATP1B1 [Similar Products]
 NCBI Official Synonym Symbols   
ATP1B
[Similar Products]
 NCBI Protein Information    sodium/potassium-transporting ATPase subunit beta-1
 UniProt Protein Name    Sodium/potassium-transporting ATPase subunit beta-1
 UniProt Synonym Protein Names   
Sodium/potassium-dependent ATPase subunit beta-1
 UniProt Gene Name    ATP1B1 [Similar Products]
 UniProt Synonym Gene Names    ATP1B [Similar Products]
 UniProt Entry Name    AT1B1_HUMAN
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 NCBI Summary for ATP1b1    The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]
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 UniProt Comments for ATP1b1    ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: basolateral plasma membrane; apical plasma membrane; plasma membrane; caveola; sodium:potassium-exchanging ATPase complex; sarcolemma; vesicle

Molecular Function: protein binding; ATPase activator activity; sodium:potassium-exchanging ATPase activity; ATPase binding

Biological Process: cellular calcium ion homeostasis; cellular sodium ion homeostasis; protein stabilization; potassium ion import; positive regulation of ATPase activity; regulation of gene expression; response to hypoxia; blood coagulation; cell adhesion; cellular potassium ion homeostasis; leukocyte migration; transmembrane transport; cardiac muscle contraction

Disease: Hypertension, Essential
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 Research Articles on ATP1b1    1. Impaired regulation and compromised activity of ion channel proteins contribute to the pathophysiology of vascular dementia.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ATP1b1 elisa kitPathways associated with ATP1b1 elisa kit
 Reference Product  PubMed Publications
 ATP1A1 elisa kit  >21 publications with ATP1b1 and ATP1A1
 ATP1A2 elisa kit  >8 publications with ATP1b1 and ATP1A2
 ATP1B3 elisa kit  >5 publications with ATP1b1 and ATP1B3
 ATP1B2 elisa kit  >5 publications with ATP1b1 and ATP1B2
 ATP1A3 elisa kit  >5 publications with ATP1b1 and ATP1A3
 FXYD2 elisa kit  >1 publications with ATP1b1 and FXYD2
 ATP1A4 elisa kit  >1 publications with ATP1b1 and ATP1A4
 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Basigin Interactions Pathway antibodies  Basigin Interactions Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
Diseases associated with ATP1b1 elisa kitOrgans/Tissues associated with ATP1b1 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >10 publications with ATP1b1 and Cardiovascular Diseases
 Hypertension Antibodies  >5 publications with ATP1b1 and Hypertension
 Kidney Diseases Antibodies  >5 publications with ATP1b1 and Kidney Diseases
 Adenocarcinoma Antibodies  >4 publications with ATP1b1 and Adenocarcinoma
 Hypertrophy Antibodies  >4 publications with ATP1b1 and Hypertrophy
 Nervous System Diseases Antibodies  >4 publications with ATP1b1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >3 publications with ATP1b1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >3 publications with ATP1b1 and Breast Neoplasms
 Fibrosis Antibodies  >3 publications with ATP1b1 and Fibrosis
 Ovarian Neoplasms Antibodies  >3 publications with ATP1b1 and Ovarian Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >27 publications with ATP1b1 and Kidney
 Muscle Antibodies  >16 publications with ATP1b1 and Muscle
 Blood Antibodies  >14 publications with ATP1b1 and Blood
 Heart Antibodies  >11 publications with ATP1b1 and Heart
 Intestine Antibodies  >7 publications with ATP1b1 and Intestine
 Brain Antibodies  >4 publications with ATP1b1 and Brain
 Stomach Antibodies  >3 publications with ATP1b1 and Stomach
 Lung Antibodies  >3 publications with ATP1b1 and Lung
 Embryonic Tissue Antibodies  >2 publications with ATP1b1 and Embryonic Tissue
 Prostate Antibodies  >2 publications with ATP1b1 and Prostate
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