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ACAT1 elisa kit :: Mouse Acetyl-CoA acetyltransferase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS285428 ACAT1 elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Acetyl-CoA acetyltransferase (ACAT1), ELISA Kit

 Also Known As   

Mouse Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) ELISA Kit

 Product Synonym Names    ACAT; MAT; T2; THIL; acetoacetyl Coenzyme A thiolase|mitochondrial acetoacetyl-CoA thiolase
 Product Gene Name   

ACAT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q8QZT1
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse ACAT1. No significant cross-reactivity or interference between Mouse ACAT1 and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ACAT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ACAT1 purchase    MBS285428 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acetyl-CoA acetyltransferase (ACAT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACAT1. The ELISA analytical biochemical technique of the MBS285428 kit is based on ACAT1 antibody-ACAT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACAT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACAT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ACAT1 elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ACAT1 in samples. An antibody specific for ACAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACAT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for ACAT1. It may not necessarily be applicable to this product.
 NCBI GI #    21450129
 NCBI GeneID    110446
 NCBI Accession #    NP_659033.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_144784.3 [Other Products]
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 UniProt Primary Accession #    Q8QZT1 [Other Products]
 UniProt Secondary Accession #    Q3TE92 [Other Products]
 UniProt Related Accession #    Q8QZT1 [Other Products]
 Molecular Weight    44,816 Da
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 NCBI Official Full Name    acetyl-CoA acetyltransferase, mitochondrial
 NCBI Official Synonym Full Names    acetyl-Coenzyme A acetyltransferase 1
 NCBI Official Symbol    Acat1 [Similar Products]
 NCBI Official Synonym Symbols   
Acat; 6330585C21Rik
[Similar Products]
 NCBI Protein Information    acetyl-CoA acetyltransferase, mitochondrial
 UniProt Protein Name    Acetyl-CoA acetyltransferase, mitochondrial
 UniProt Synonym Protein Names   
Acetoacetyl-CoA thiolase
 Protein Family    Acetyl-CoA acetyltransferase
 UniProt Gene Name    Acat1 [Similar Products]
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 UniProt Comments for ACAT1    ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.

Protein type: Acetyltransferase; Amino Acid Metabolism - lysine degradation; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 2.3.1.9; Lipid Metabolism - fatty acid; Lipid Metabolism - synthesis and degradation of ketone bodies; Mitochondrial; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis

Chromosomal Location of Human Ortholog: 9 A5.3|9 29.12 cM

Cellular Component: extracellular exosome; mitochondrial inner membrane; mitochondrial matrix; mitochondrion

Molecular Function: acetyl-CoA C-acetyltransferase activity; carbon-carbon lyase activity; catalytic activity; coenzyme binding; enzyme binding; ligase activity, forming carbon-carbon bonds; metal ion binding; protein homodimerization activity; transferase activity; transferase activity, transferring acyl groups; transferase activity, transferring groups other than amino-acyl groups

Biological Process: acetyl-CoA biosynthetic process; acetyl-CoA catabolic process; coenzyme A biosynthetic process; coenzyme A metabolic process; fatty acid beta-oxidation; isoleucine catabolic process; ketone body catabolic process; metabolic process; protein homooligomerization
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 Research Articles on ACAT1    1. Data show that microRNA miR-467b regulates the acetyl-CoA acetyltransferase 1 (ACAT1) expression via targeting ACAT1 3' untranslated regions (3'UTR).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ACAT1 elisa kitPathways associated with ACAT1 elisa kit
 Reference Product  PubMed Publications
 Hmgcs2 elisa kit  >6 publications with ACAT1 and Hmgcs2
 Oxct1 elisa kit  >5 publications with ACAT1 and Oxct1
 Ehhadh elisa kit  >2 publications with ACAT1 and Ehhadh
 Acacb elisa kit  >1 publications with ACAT1 and Acacb
 Hadha elisa kit  >1 publications with ACAT1 and Hadha
 Products by Pathway  Pathway Diagram
 2-methylbutyrate Biosynthesis Pathway antibodies  2-methylbutyrate Biosynthesis Pathway Diagram
 Branched-chain Amino Acid Catabolism Pathway antibodies  Branched-chain Amino Acid Catabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
Diseases associated with ACAT1 elisa kitOrgans/Tissues associated with ACAT1 elisa kit
 Disease Name  Pubmed Publications
 Atherosclerosis Antibodies  >88 publications with ACAT1 and Atherosclerosis
 Cardiovascular Diseases Antibodies  >61 publications with ACAT1 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >26 publications with ACAT1 and Nervous System Diseases
 Hypercholesterolemia Antibodies  >17 publications with ACAT1 and Hypercholesterolemia
 Inflammation Antibodies  >15 publications with ACAT1 and Inflammation
 Fatty Liver Antibodies  >15 publications with ACAT1 and Fatty Liver
 Liver Diseases Antibodies  >15 publications with ACAT1 and Liver Diseases
 Dyslipidemias Antibodies  >15 publications with ACAT1 and Dyslipidemias
 Hyperlipidemias Antibodies  >14 publications with ACAT1 and Hyperlipidemias
 Necrosis Antibodies  >11 publications with ACAT1 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >59 publications with ACAT1 and Blood
 Liver Antibodies  >53 publications with ACAT1 and Liver
 Intestine Antibodies  >22 publications with ACAT1 and Intestine
 Brain Antibodies  >19 publications with ACAT1 and Brain
 Muscle Antibodies  >16 publications with ACAT1 and Muscle
 Bone Antibodies  >13 publications with ACAT1 and Bone
 Bone Marrow Antibodies  >10 publications with ACAT1 and Bone Marrow
 Ovary Antibodies  >8 publications with ACAT1 and Ovary
 Heart Antibodies  >8 publications with ACAT1 and Heart
 Kidney Antibodies  >7 publications with ACAT1 and Kidney
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