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AgRP elisa kit :: Mouse AgRP ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2509262
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
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 Product Name   


★Popular Item★
 Also Known As   

Mouse AgRP (Agouti Related Protein) ELISA Kit

 Product Gene Name   

AgRP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601665
 3D Structure    ModBase 3D Structure for O00253
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse AgRP. No significant cross-reactivity or interference between Mouse AgRP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse AgRP and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    Min: 9.375pg/mL; Max: 1000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AgRP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AgRP purchase    MBS2509262 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AgRP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing AgRP, with an estimated sensitivity of Min: 9.375pg/mL; Max: 1000pg/mL. The ELISA analytical biochemical technique of the MBS2509262 kit is based on AgRP antibody-AgRP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AgRP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AgRP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AgRP elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AgRP in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AgRP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse AgRP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AgRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse AgRP. You can calculate the concentration of Mouse AgRP in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of AgRP elisa kit    AgRP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2509262. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AgRP. It may not necessarily be applicable to this product.
 NCBI GI #    4501995
 NCBI GeneID    181
 NCBI Accession #    NP_001129.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001138.1 [Other Products]
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 UniProt Primary Accession #    O00253 [Other Products]
 UniProt Secondary Accession #    O15459; Q2TBD9 [Other Products]
 UniProt Related Accession #    O00253 [Other Products]
 Molecular Weight    14,440 Da
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 NCBI Official Full Name    agouti-related protein
 NCBI Official Synonym Full Names    agouti related neuropeptide
 NCBI Official Symbol    AGRP [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    agouti-related protein; agouti related protein homolog
 UniProt Protein Name    Agouti-related protein
 Protein Family    Agouti-related protein
 UniProt Gene Name    AGRP [Similar Products]
 UniProt Synonym Gene Names    AGRT; ART [Similar Products]
 UniProt Entry Name    AGRP_HUMAN
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 NCBI Summary for AgRP    This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]
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 UniProt Comments for AgRP    AGRP: Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner. Genetic variations in AGRP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: extracellular space; cell soma; Golgi lumen

Molecular Function: neuropeptide hormone activity; receptor binding

Biological Process: maternal process involved in pregnancy; hormone-mediated signaling; eating behavior; neuropeptide signaling pathway; photoperiodism; feeding behavior; adult feeding behavior; response to insulin stimulus

Disease: Obesity
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 Research Articles on AgRP    1. Elevations in AgRP also favor the diagnosis of ectopic ACTH syndrome, suggesting AgRP should be further evaluated as a potential neuroendocrine tumor marker.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AgRP elisa kitPathways associated with AgRP elisa kit
 Reference Product  PubMed Publications
 POMC elisa kit  >564 publications with AgRP and POMC
 MC4R elisa kit  >132 publications with AgRP and MC4R
 MC3R elisa kit  >62 publications with AgRP and MC3R
 FOS elisa kit  >39 publications with AgRP and FOS
 TRH elisa kit  >32 publications with AgRP and TRH
 FOXO1 elisa kit  >17 publications with AgRP and FOXO1
 LEP elisa kit  >10 publications with AgRP and LEP
 SDC3 elisa kit  >3 publications with AgRP and SDC3
 GHRL elisa kit  >3 publications with AgRP and GHRL
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Syndecan-3-mediated Signaling Events Pathway antibodies  Syndecan-3-mediated Signaling Events Pathway Diagram
Diseases associated with AgRP elisa kitOrgans/Tissues associated with AgRP elisa kit
 Disease Name  Pubmed Publications
 Weight Gain Antibodies  >120 publications with AgRP and Weight Gain
 Weight Loss Antibodies  >94 publications with AgRP and Weight Loss
 Insulin Resistance Antibodies  >59 publications with AgRP and Insulin Resistance
 Disease Models, Animal Antibodies  >57 publications with AgRP and Disease Models, Animal
 Hyperglycemia Antibodies  >27 publications with AgRP and Hyperglycemia
 Inflammation Antibodies  >26 publications with AgRP and Inflammation
 Nervous System Diseases Antibodies  >21 publications with AgRP and Nervous System Diseases
 Fatty Liver Antibodies  >12 publications with AgRP and Fatty Liver
 Necrosis Antibodies  >10 publications with AgRP and Necrosis
 Cardiovascular Diseases Antibodies  >9 publications with AgRP and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >69 publications with AgRP and Connective Tissue
 Kidney Antibodies  >18 publications with AgRP and Kidney
 Lung Antibodies  >3 publications with AgRP and Lung
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