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ADH1 elisa kit :: Mouse Alcohol Dehydrogenase 1 (ADH1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023380 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $260 +1 FREE 8GB USB
48-Strip-Wells  /  $465 +1 FREE 8GB USB
96-Strip-Wells  /  $625 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,530 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,565 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alcohol Dehydrogenase 1 (ADH1), ELISA Kit

 Also Known As   

Alcohol Dehydrogenase 1 (ADH1) ELISA Kit

 Product Synonym Names    ADH1A; Alcohol Dehydrogenase 1A(Class I)Alpha Polypeptide; Alcohol dehydrogenase subunit alpha
 Product Gene Name   

ADH1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P06525
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Alcohol Dehydrogenase 1 (ADH1).
No significant cross-reactivity or interference between Alcohol Dehydrogenase 1 (ADH1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.133ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Alcohol Dehydrogenase 1 (ADH1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Alcohol Dehydrogenase 1 (ADH1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADH1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADH1 purchase    MBS2023380 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alcohol Dehydrogenase 1 (ADH1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing ADH1, with an estimated sensitivity of < 0.133ng/mL. The ELISA analytical biochemical technique of the MBS2023380 kit is based on ADH1 antibody-ADH1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADH1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADH1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADH1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alcohol Dehydrogenase 1 (ADH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alcohol Dehydrogenase 1 (ADH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alcohol Dehydrogenase 1 (ADH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alcohol Dehydrogenase 1 (ADH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for ADH1 elisa kit    Enzyme & Kinase; Metabolic pathway; Hepatology
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 Typical Testing Data/Standard Curve (for reference only) of ADH1 elisa kit    ADH1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2023380. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADH1. It may not necessarily be applicable to this product.
 NCBI GI #    15223838
 NCBI GeneID    844047
 NCBI Accession #    NP_177837.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_106362.2 [Other Products]
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 UniProt Primary Accession #    P06525 [Other Products]
 UniProt Secondary Accession #    O04080; O04713; O04717; O04868; O23821; Q8LA61; Q94AY6; Q9CAZ2; Q9CAZ3 [Other Products]
 UniProt Related Accession #    P06525 [Other Products]
 Molecular Weight    41,178 Da
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 NCBI Official Full Name    alcohol dehydrogenase 1
 NCBI Official Symbol    ADH1 [Similar Products]
 NCBI Official Synonym Symbols   
ADH; ADH1; ALCOHOL DEHYDROGENASE; alcohol dehydrogenase 1; ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE; ATADH; ATADH1; F22K20.19; F22K20_19
[Similar Products]
 NCBI Protein Information    alcohol dehydrogenase 1
 UniProt Protein Name    Alcohol dehydrogenase class-P
 Protein Family    Alcohol dehydrogenase
 UniProt Gene Name    ADH1 [Similar Products]
 UniProt Synonym Gene Names    ADH [Similar Products]
 UniProt Entry Name    ADH1_ARATH
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 NCBI Summary for ADH1    Catalyzes the reduction of acetaldehyde using NADH as reductant. Requires zinc for activity. Dimer. Anaerobic response polypeptide (ANP). Fermentation. The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide.
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 UniProt Comments for ADH1    Alcohol dehydrogenase mostly active on ethanol (EtOH), but exhibits broad substrates selectivity for primary and secondary alcohols (e.g. butanol, propyl alcohol, pentanol, isopentanol, ethylene glycol, isopropanol, methanol and tertiary butyl alcohol) (PubMed:23707506). Converts allyl alcohol to highly toxic acryl-aldehyde (PubMed:20508152). Required for survival and acclimation in hypoxic conditions, especially in roots (PubMed:12857811, PubMed:9880346).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ADH1 elisa kitOrgans/Tissues associated with ADH1 elisa kit
 Products by Pathway  Pathway Diagram
 Biosynthesis Of Secondary Metabolites Pathway antibodies  Biosynthesis Of Secondary Metabolites Pathway Diagram
 Degradation Of Aromatic Compounds Pathway antibodies  Degradation Of Aromatic Compounds Pathway Diagram
 Degradation Of Aromatic Compounds Pathway antibodies  Degradation Of Aromatic Compounds Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Other Antibodies  >165 publications with ADH1 and Other
 Root Antibodies  >41 publications with ADH1 and Root
 Cell Culture Antibodies  >24 publications with ADH1 and Cell Culture
 Leaf Antibodies  >23 publications with ADH1 and Leaf
 Flower Antibodies  >12 publications with ADH1 and Flower
 Whole Plant Antibodies  >2 publications with ADH1 and Whole Plant
 Bud Antibodies  >1 publications with ADH1 and Bud
 Vegetative Tissue Antibodies  >1 publications with ADH1 and Vegetative Tissue
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