Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow ACER1 rightarrow Mouse ACER1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Platelet Derived Growth Factor Bb (PDGF-BB) ELISA Kit
 • Actin Alpha 1, Skeletal Muscle (ACTalpha1) ELISA Kit
 • AFP Antibody
 • c-PLA2 (Phospho-Ser505) Antibody
 • HeLa Slide
 • Adenovirus Antibody
 • N-Acetylglucosamine Kinase (NAGK) Antibody
 • Serine-threonine-protein kinase mTOR ELISA Kit
 • factor XII IgG Antibody
 • MUC1 Antibody
 • Varicella-Zoster Virus (VZV) Antigens Antigen
 • Vitamin D Binding Protein (DBP) ELISA Kit
 • RAD9A Antibody
 • Peroxisome Proliferator Activated Receptor Alpha (PPAR-alpha... ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

ACER1 elisa kit :: Mouse Alkaline ceramidase 1 (ACER1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7235867
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alkaline ceramidase 1 (ACER1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Alkaline ceramidase 1 (ACER1) ELISA Kit

 Product Gene Name   

ACER1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    613491
Section Bar
 Species Reactivity    Mouse
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of ACER1. No significant cross-reactivity or interference between ACER1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACER1 and all the analogues, therefore, cross reaction may still exist in some cases.
Section Bar
 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive (Quantitative)
 Sensitivity    0.1 ng/mL.
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ACER1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ACER1 purchase    MBS7235867 is a ready-to-use microwell, strip plate Competitive (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alkaline ceramidase 1 (ACER1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACER1, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7235867 kit is based on ACER1 antibody-ACER1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACER1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACER1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for ACER1 elisa kit

   Intended Uses: This ACER1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACER1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ACER1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACER1 antibody and an ACER1-HRP conjugate. The assay sample and buffer are incubated together with ACER1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACER1 concentration since ACER1 from samples and ACER1-HRP conjugate compete for the anti-ACER1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACER1 from the sample, fewer sites are left to bind ACER1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACER1 concentration in each sample is interpolated from this standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of ACER1 elisa kit    ACER1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS7235867. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ACER1. It may not necessarily be applicable to this product.
 NCBI GI #    19424128
 NCBI GeneID    125981
 NCBI Accession #    NP_597999.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_133492.2 [Other Products]
Section Bar
 UniProt Related Accession #    Q8TDN7 [Other Products]
 Molecular Weight    31,095 Da
Table BarTOPTable Bar
 NCBI Official Full Name    alkaline ceramidase 1
 NCBI Official Synonym Full Names    alkaline ceramidase 1
 NCBI Official Symbol    ACER1 [Similar Products]
 NCBI Official Synonym Symbols   
ASAH3; ALKCDase1
[Similar Products]
 NCBI Protein Information    alkaline ceramidase 1; alkCDase 1; alkaline CDase 1; acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3; N-acylsphingosine amidohydrolase (alkaline ceramidase) 3
 UniProt Protein Name    Alkaline ceramidase 1
 UniProt Synonym Protein Names   
Acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3
 Protein Family    Alkaline ceramidase
 UniProt Gene Name    ACER1 [Similar Products]
 UniProt Synonym Gene Names    ASAH3; AlkCDase 1; Alkaline CDase 1 [Similar Products]
 UniProt Entry Name    ACER1_HUMAN
Section Bar
 NCBI Summary for ACER1    Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 [PubMed 16477081]; Sun et al., 2008 [PubMed 17713573]).[supplied by OMIM, Jul 2010]
Section Bar
 UniProt Comments for ACER1    ACER1: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo- phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids. Belongs to the alkaline ceramidase family.

Protein type: Hydrolase; Lipid Metabolism - sphingolipid; EC 3.5.1.23; Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: ceramidase activity

Biological Process: keratinocyte differentiation; epidermis development; regulation of lipid metabolic process; sphingolipid metabolic process; response to alkalinity; sphingolipid biosynthetic process; sphingosine biosynthetic process; ceramide catabolic process; cell differentiation
Table BarTOPTable Bar
 Research Articles on ACER1    1. upregulation of haCER1 and AC mediates the Ca2+(o)-induced growth arrest and differentiation of keratinocytes by generating sphingosine and its phosphate
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with ACER1 elisa kitOrgans/Tissues associated with ACER1 elisa kit
 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Sphingolipid De Novo Biosynthesis Pathway antibodies  Sphingolipid De Novo Biosynthesis Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingosine Biosynthesis Pathway antibodies  Sphingosine Biosynthesis Pathway Diagram
 Sphingosine Biosynthesis Pathway antibodies  Sphingosine Biosynthesis Pathway Diagram
 Sphingosine And Sphingosine-1-phosphate Metabolism Pathway antibodies  Sphingosine And Sphingosine-1-phosphate Metabolism Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >4 publications with ACER1 and Skin
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions