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ACER2 elisa kit :: Mouse Alkaline ceramidase 2 (ACER2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7209609
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
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 Product Name   

Alkaline ceramidase 2 (ACER2), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Alkaline ceramidase 2 (ACER2) ELISA Kit

 Product Gene Name   

ACER2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    613492
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ACER2. No significant cross-reactivity or interference between ACER2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACER2 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive (Quantitative)
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ACER2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ACER2 purchase    MBS7209609 is a ready-to-use microwell, strip plate Competitive (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alkaline ceramidase 2 (ACER2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACER2, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7209609 kit is based on ACER2 antibody-ACER2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACER2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACER2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ACER2 elisa kit

   Intended Uses: This ACER2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACER2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ACER2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACER2 antibody and an ACER2-HRP conjugate. The assay sample and buffer are incubated together with ACER2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACER2 concentration since ACER2 from samples and ACER2-HRP conjugate compete for the anti-ACER2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACER2 from the sample, fewer sites are left to bind ACER2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACER2 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ACER2 elisa kit    ACER2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for ACER2. It may not necessarily be applicable to this product.
 NCBI GI #    71043498
 NCBI GeneID    340485
 NCBI Accession #    NP_001010887.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001010887.2 [Other Products]
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 UniProt Secondary Accession #    Q569G5; Q5VZR7; Q71RD2; A2A3R8 [Other Products]
 UniProt Related Accession #    Q5QJU3 [Other Products]
 Molecular Weight    15,864 Da
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 NCBI Official Full Name    alkaline ceramidase 2
 NCBI Official Synonym Full Names    alkaline ceramidase 2
 NCBI Official Symbol    ACER2 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    alkaline ceramidase 2; haCER2; alkCDase 2; alkaline CDase 2; ceramide hydrolase
 UniProt Protein Name    Alkaline ceramidase 2
 UniProt Synonym Protein Names   
Acylsphingosine deacylase 3-like; N-acylsphingosine amidohydrolase 3-like
 Protein Family    Alkaline ceramidase
 UniProt Gene Name    ACER2 [Similar Products]
 UniProt Synonym Gene Names    ASAH3L; PP11646; AlkCDase 2; Alkaline CDase 2; haCER2 [Similar Products]
 UniProt Entry Name    ACER2_HUMAN
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 NCBI Summary for ACER2    The sphingolipid metabolite sphingosine-1-phosphate promotes cell proliferation and survival, whereas its precursor, sphingosine, has the opposite effect. The ceramidase ACER2 hydrolyzes very long chain ceramides to generate sphingosine (Xu et al., 2006 [PubMed 16940153]).[supplied by OMIM, Jul 2010]
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 UniProt Comments for ACER2    ACER2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. The substrate preference is D-erythro-C(18:1)-, C(20:1)-, C(20:4)-ceramide > D-erythro-C(16:0)-, C(18:0), C(20:0)- ceramide > D-erythro-C(24:1)-ceramide > D-erythro-C(12:0)- ceramide, D-erythro-C(14:0)-ceramides > D-erythro-C(24:0)-ceramide > D-erythro-C(6:0)-ceramide. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate. Belongs to the alkaline ceramidase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Lipid Metabolism - sphingolipid; EC; Hydrolase

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: Golgi membrane; Golgi apparatus; integral to Golgi membrane

Molecular Function: ceramidase activity

Biological Process: caspase activation; response to retinoic acid; sphingolipid metabolic process; sphingolipid biosynthetic process; positive regulation of cell proliferation; sphingosine biosynthetic process; negative regulation of cell-matrix adhesion; ceramide metabolic process; negative regulation of cell adhesion mediated by integrin
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 Research Articles on ACER2    1. c-Src-mediated signal positively regulates ACER activity in a Ca2+-independent manner.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ACER2 elisa kitDiseases associated with ACER2 elisa kit
 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Sphingolipid De Novo Biosynthesis Pathway antibodies  Sphingolipid De Novo Biosynthesis Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingosine Biosynthesis Pathway antibodies  Sphingosine Biosynthesis Pathway Diagram
 Sphingosine Biosynthesis Pathway antibodies  Sphingosine Biosynthesis Pathway Diagram
 Sphingosine And Sphingosine-1-phosphate Metabolism Pathway antibodies  Sphingosine And Sphingosine-1-phosphate Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >2 publications with ACER2 and Fatty Liver
 Skin Neoplasms Antibodies  >1 publications with ACER2 and Skin Neoplasms
 Nervous System Diseases Antibodies  >1 publications with ACER2 and Nervous System Diseases
 Brain Diseases Antibodies  >1 publications with ACER2 and Brain Diseases
 Liver Diseases Antibodies  >1 publications with ACER2 and Liver Diseases
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