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TMEM195 elisa kit :: Mouse Alkylglycerol monooxygenase (TMEM195) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7244380
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alkylglycerol monooxygenase (TMEM195), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Alkylglycerol monooxygenase (TMEM195) ELISA Kit

 Product Gene Name   

TMEM195 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    613738
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of TMEM195. No significant cross-reactivity or interference between TMEM195 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TMEM195 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TMEM195 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TMEM195 purchase    MBS7244380 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alkylglycerol monooxygenase (TMEM195) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TMEM195, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7244380 kit is based on TMEM195 antibody-TMEM195 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TMEM195 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TMEM195. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TMEM195 elisa kit

   Intended Uses: This TMEM195 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TMEM195. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: TMEM195 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TMEM195 antibody and an TMEM195-HRP conjugate. The assay sample and buffer are incubated together with TMEM195-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TMEM195 concentration since TMEM195 from samples and TMEM195-HRP conjugate compete for the anti-TMEM195 antibody binding site. Since the number of sites is limited, as more sites are occupied by TMEM195 from the sample, fewer sites are left to bind TMEM195-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMEM195 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for TMEM195 elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of TMEM195 elisa kit    TMEM195 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7244380. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TMEM195. It may not necessarily be applicable to this product.
 NCBI GI #    51972212
 NCBI GeneID    392636
 NCBI Accession #    NP_001004320.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001004320.1 [Other Products]
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 UniProt Secondary Accession #    A4D114; A6NCH5 [Other Products]
 UniProt Related Accession #    Q6ZNB7 [Other Products]
 Molecular Weight    51,500 Da
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 NCBI Official Full Name    alkylglycerol monooxygenase
 NCBI Official Synonym Full Names    alkylglycerol monooxygenase
 NCBI Official Symbol    AGMO [Similar Products]
 NCBI Official Synonym Symbols   
TMEM195
[Similar Products]
 NCBI Protein Information    alkylglycerol monooxygenase; transmembrane protein 195; glyceryl-ether monooxygenase
 UniProt Protein Name    Alkylglycerol monooxygenase
 UniProt Synonym Protein Names   
Transmembrane protein 195
 UniProt Gene Name    AGMO [Similar Products]
 UniProt Synonym Gene Names    TMEM195 [Similar Products]
 UniProt Entry Name    ALKMO_HUMAN
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 NCBI Summary for TMEM195    The protein encoded by this gene is a tetrahydrobiopterin- and iron-dependent enzyme that cleaves the ether bond of alkylglycerols. Sequence comparisons distinguish this protein as forming a third, distinct class of tetrahydrobiopterin-dependent enzymes. Variations in this gene have been associated with decreased glucose-stimulated insulin response, type 2 diabetes, and susceptibility to intracranial aneurysms. [provided by RefSeq, Aug 2012]
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 UniProt Comments for TMEM195    AGMO: Glyceryl-ether monooxygenase that cleaves the O-alkyl bond of ether lipids. Ether lipids are essential components of brain membranes. Belongs to the sterol desaturase family. TMEM195 subfamily.

Protein type: Oxidoreductase; Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral; EC 1.14.16.5

Chromosomal Location of Human Ortholog: 7p21.2

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: iron ion binding; glyceryl-ether monooxygenase activity

Biological Process: ether lipid metabolic process; membrane lipid metabolic process; fatty acid biosynthetic process
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 Research Articles on TMEM195    1. Among all of the acidic residues within the eight-histidine motif, only mutation of Glu137 to alanine led to an 18-fold increase in the Michaelis-Menten constant for tetrahydrobiopterin, suggesting a role in tetrahydrobiopterin interaction
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TMEM195 elisa kitDiseases associated with TMEM195 elisa kit
 Reference Product  PubMed Publications
 GLIS3 elisa kit  >1 publications with TMEM195 and GLIS3
 DGKB elisa kit  >1 publications with TMEM195 and DGKB
 Disease Name  Pubmed Publications
 Learning Disorders Antibodies  >3 publications with TMEM195 and Learning Disorders
 Disease Models, Animal Antibodies  >1 publications with TMEM195 and Disease Models, Animal
 Hypertrophy Antibodies  >1 publications with TMEM195 and Hypertrophy
Organs/Tissues associated with TMEM195 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >34 publications with TMEM195 and Brain
 Muscle Antibodies  >4 publications with TMEM195 and Muscle
 Testis Antibodies  >1 publications with TMEM195 and Testis
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