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a2M elisa kit :: Mouse Alpha-2-Macroglobulin (a2M) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020230 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $230 +1 FREE 8GB USB
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $505 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,020 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,640 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alpha-2-Macroglobulin (a2M), ELISA Kit

★Popular Item★
 Also Known As   

Alpha-2-Macroglobulin (a2M) ELISA Kit

 Product Synonym Names    A2MG; A2mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
 Product Gene Name   

a2M elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    103950
 3D Structure    ModBase 3D Structure for P01023
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of a2M. No significant cross-reactivity or interference between a2M and analogues was observed.
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 Samples    Serum, plasma, urine and other biological fluids
 Assay Type    Quantitative Competitive
 Detection Range    3.70-300ng/mL
 Sensitivity    <1.55ng/mL.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level a2M were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level a2M were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of a2M elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for a2M purchase    MBS2020230 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-2-Macroglobulin (a2M) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.70-300ng/mL in biological research samples containing a2M, with an estimated sensitivity of <1.55ng/mL. The ELISA analytical biochemical technique of the MBS2020230 kit is based on a2M antibody-a2M antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect a2M antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, a2M. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, urine and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for a2M elisa kit

   Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of a2M in mouse serum, plasma, urine and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to a2M has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled a2M and unlabeled a2M (Standards or samples) with the pre-coated antibody specific to a2M. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of a2M in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of a2M in the sample.
 Product Categories/Family for a2M elisa kit    Tumor immunity; Infection immunity; Hematology
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  Typical Testing Data/Standard Curve (for reference only) of a2M elisa kit    a2M elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020230. Click to request current manual
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NCBI/Uniprot data below describe general gene information for a2M. It may not necessarily be applicable to this product.
 NCBI GI #    66932947
 NCBI GeneID    2
 NCBI Accession #    NP_000005.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000014.4 [Other Products]
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 UniProt Primary Accession #    P01023 [Other Products]
 UniProt Secondary Accession #    Q13677; Q59F47; Q5QTS0; Q68DN2; Q6PIY3; Q6PN97 [Other Products]
 UniProt Related Accession #    P01023 [Other Products]
 Molecular Weight    163,291 Da
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 NCBI Official Full Name    alpha-2-macroglobulin
 NCBI Official Synonym Full Names    alpha-2-macroglobulin
 NCBI Official Symbol    A2M [Similar Products]
 NCBI Official Synonym Symbols   
A2MD; CPAMD5; FWP007; S863-7
[Similar Products]
 NCBI Protein Information    alpha-2-macroglobulin
 UniProt Protein Name    Alpha-2-macroglobulin
 UniProt Synonym Protein Names   
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
 Protein Family    Alpha-2-macroglobulin
 UniProt Gene Name    A2M [Similar Products]
 UniProt Synonym Gene Names    CPAMD5; Alpha-2-M [Similar Products]
 UniProt Entry Name    A2MG_HUMAN
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 NCBI Summary for a2M    Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]
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 UniProt Comments for a2M    A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular region; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-8 binding; interleukin-1 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding

Biological Process: negative regulation of complement activation, lectin pathway; extracellular matrix disassembly; platelet activation; extracellular matrix organization and biogenesis; regulation of small GTPase mediated signal transduction; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway

Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency
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 Research Articles on a2M    1. phosphorylating the T-loop Akt residue Thr(308) by PDK1 requires Raptor of the mTORC1 complex as a platform or scaffold protein.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with a2M elisa kitPathways associated with a2M elisa kit
 Reference Product  PubMed Publications
 APP elisa kit  >17 publications with a2M and APP
 LRP1 elisa kit  >12 publications with a2M and LRP1
 ALB elisa kit  >6 publications with a2M and ALB
 APOA1 elisa kit  >4 publications with a2M and APOA1
 PLG elisa kit  >2 publications with a2M and PLG
 TGFB1 elisa kit  >2 publications with a2M and TGFB1
 SERPINA1 elisa kit  >1 publications with a2M and SERPINA1
 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 HDL-mediated Lipid Transport Pathway antibodies  HDL-mediated Lipid Transport Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Intrinsic Pathway antibodies  Intrinsic Pathway Diagram
Diseases associated with a2M elisa kitOrgans/Tissues associated with a2M elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >95 publications with a2M and Nervous System Diseases
 Mental Disorders Antibodies  >83 publications with a2M and Mental Disorders
 Alzheimer Disease Antibodies  >81 publications with a2M and Alzheimer Disease
 Inflammation Antibodies  >32 publications with a2M and Inflammation
 Necrosis Antibodies  >14 publications with a2M and Necrosis
 Liver Diseases Antibodies  >14 publications with a2M and Liver Diseases
 Lung Diseases Antibodies  >13 publications with a2M and Lung Diseases
 Disease Models, Animal Antibodies  >11 publications with a2M and Disease Models, Animal
 Fibrosis Antibodies  >11 publications with a2M and Fibrosis
 Carcinoma Antibodies  >8 publications with a2M and Carcinoma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >127 publications with a2M and Blood
 Liver Antibodies  >38 publications with a2M and Liver
 Brain Antibodies  >31 publications with a2M and Brain
 Vascular Antibodies  >15 publications with a2M and Vascular
 Lung Antibodies  >13 publications with a2M and Lung
 Heart Antibodies  >9 publications with a2M and Heart
 Prostate Antibodies  >9 publications with a2M and Prostate
 Uterus Antibodies  >6 publications with a2M and Uterus
 Ovary Antibodies  >6 publications with a2M and Ovary
 Embryonic Tissue Antibodies  >6 publications with a2M and Embryonic Tissue
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