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AFA elisa kit :: Mouse Anti-Filaggrin Antibody ELISA Kit

Scan QR to view Datasheet Catalog #    MBS724801 AFA elisa kit
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Anti-Filaggrin Antibody (AFA), ELISA Kit

 Also Known As   

Mouse Anti-Filaggrin Antibody ELISA Kit

 Product Gene Name   

AFA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    605803
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 Species Reactivity    Mouse
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0-250ng/mL
 Sensitivity    1.0ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AFA purchase    MBS724801 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Filaggrin Antibody (AFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0-250ng/mL in biological research samples containing AFA, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS724801 kit is based on AFA antibody-AFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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 Product Categories/Family for AFA elisa kit    Immunology
NCBI/Uniprot data below describe general gene information for AFA. It may not necessarily be applicable to this product.
 NCBI GI #    60097902
 NCBI GeneID    2312
 NCBI Accession #    NP_002007.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002016.1 [Other Products]
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 UniProt Secondary Accession #    Q01720; Q5T583; Q9UC71 [Other Products]
 UniProt Related Accession #    P20930 [Other Products]
 Molecular Weight    435,170 Da
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 NCBI Official Full Name    filaggrin
 NCBI Official Synonym Full Names    filaggrin
 NCBI Official Symbol    FLG [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    filaggrin; epidermal filaggrin
 UniProt Protein Name    Filaggrin
 UniProt Gene Name    FLG [Similar Products]
 UniProt Entry Name    FILA_HUMAN
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 NCBI Summary for AFA    The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]
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 UniProt Comments for AFA    FLG: Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. Defects in FLG are the cause of ichthyosis vulgaris (VI); also known as ichthyosis simplex. Ichthyosis vulgaris is the most common form of ichthyosis inherited as an autosomal dominant trait. It is characterized by palmar hyperlinearity, keratosis pilaris and a fine scale that is most prominent over the lower abdomen, arms, and legs. Ichthyosis vulgaris is characterized histologically by absent or reduced keratohyalin granules in the epidermis and mild hyperkeratosis. The disease can be associated with frequent asthma, eczema or hay fever. Defects in FLG are a cause of susceptibility to dermatitis atopic type 2 (ATOD2). Atopic dermatitis is a complex, inflammatory disease with multiple alleles at several loci thought to be involved in the pathogenesis. It commonly begins in infancy or early childhood and is characterized by a chronic relapsing form of skin inflammation, a disturbance of epidermal barrier function that culminates in dry skin, and IgE- mediated sensitization to food and environmental allergens. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee. Belongs to the S100-fused protein family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoplasmic membrane-bound vesicle; intermediate filament; nucleus

Molecular Function: protein binding; calcium ion binding; structural molecule activity

Biological Process: keratinocyte differentiation; multicellular organismal development

Disease: Ichthyosis Vulgaris; Dermatitis, Atopic, 2
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 Research Articles on AFA    1. Sensitization for type I allergens did not differ between FLG loss-of-function mutation carriers and wild-type subjects. For type IV allergens, more FLG loss-of-function carriers were sensitized to lanolin and p-tert-butylphenol-formaldehyde resin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AFA elisa kitPathways associated with AFA elisa kit
 Reference Product  PubMed Publications
 FGFR1 elisa kit  >38 publications with AFA and FGFR1
 LOR elisa kit  >11 publications with AFA and LOR
 SPINK5 elisa kit  >7 publications with AFA and SPINK5
 IVL elisa kit  >7 publications with AFA and IVL
 EGF elisa kit  >5 publications with AFA and EGF
 KRT1 elisa kit  >1 publications with AFA and KRT1
 Products by Pathway  Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
Diseases associated with AFA elisa kitOrgans/Tissues associated with AFA elisa kit
 Disease Name  Pubmed Publications
 Dermatitis, Atopic Antibodies  >179 publications with AFA and Dermatitis, Atopic
 Dermatitis, Atopic, 1 Antibodies  >101 publications with AFA and Dermatitis, Atopic, 1
 Dermatitis, Atopic, 2 Antibodies  >87 publications with AFA and Dermatitis, Atopic, 2
 Eczema Antibodies  >74 publications with AFA and Eczema
 Ichthyosis Antibodies  >66 publications with AFA and Ichthyosis
 Ichthyosis Vulgaris Antibodies  >62 publications with AFA and Ichthyosis Vulgaris
 Inflammation Antibodies  >33 publications with AFA and Inflammation
 Lung Diseases Antibodies  >30 publications with AFA and Lung Diseases
 Psoriasis Antibodies  >14 publications with AFA and Psoriasis
 Necrosis Antibodies  >12 publications with AFA and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >194 publications with AFA and Skin
 Brain Antibodies  >31 publications with AFA and Brain
 Uterus Antibodies  >4 publications with AFA and Uterus
 Mouth Antibodies  >2 publications with AFA and Mouth
 Testis Antibodies  >1 publications with AFA and Testis
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