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AQP-4 elisa kit :: Mouse Anti-aquaporin 4 antibody ELISA Kit

Scan QR to view Datasheet Catalog #    MBS746880
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Anti-aquaporin 4 antibody (AQP-4), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Anti-aquaporin 4 antibody ELISA Kit

 Product Gene Name   

AQP-4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Anti-AQP4. No significant cross-reactivity or interference between Anti-AQP4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Anti-AQP4 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    5-100ng/mL
 Sensitivity    1.0ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AQP-4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AQP-4 purchase    MBS746880 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-aquaporin 4 antibody (AQP-4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5-100ng/mL in biological research samples containing AQP-4, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS746880 kit is based on AQP-4 antibody-AQP-4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AQP-4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AQP-4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AQP-4 elisa kit

   Intended Uses: This Anti-AQP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Anti-AQP4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: Anti-AQP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing Aquaporin 4 antigen and an Anti-AQP4-HRP conjugate. The assay sample and buffer are incubated together with Anti-AQP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-AQP4 concentration since Anti-AQP4 from samples and Anti-AQP4-HRP conjugate compete for the Aquaporin 4 antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-AQP4 from the sample, fewer sites are left to bind Anti-AQP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-AQP4 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for AQP-4 elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of AQP-4 elisa kit    AQP-4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS746880. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AQP-4. It may not necessarily be applicable to this product.
 NCBI GI #    160415211
 NCBI GeneID    11829
 NCBI Accession #    NP_033830.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_009700.2 [Other Products]
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 UniProt Secondary Accession #    P97818; Q4FJP1; Q61131; Q61132; Q8VHE4; Q8VHE5; Q9EQI3 [Other Products]
 UniProt Related Accession #    P55088 [Other Products]
 Molecular Weight    37,977 Da
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 NCBI Official Full Name    aquaporin-4
 NCBI Official Synonym Full Names    aquaporin 4
 NCBI Official Symbol    Aqp4 [Similar Products]
 NCBI Official Synonym Symbols   
WCH4
[Similar Products]
 NCBI Protein Information    aquaporin-4; AQP-4; mercurial-insensitive water channel
 UniProt Protein Name    Aquaporin-4
 UniProt Synonym Protein Names   
Mercurial-insensitive water channel; MIWC; WCH4
 UniProt Gene Name    Aqp4 [Similar Products]
 UniProt Synonym Gene Names    AQP-4; MIWC [Similar Products]
 UniProt Entry Name    AQP4_MOUSE
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 UniProt Comments for AQP-4    AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described

Protein type: Membrane protein, integral; Channel, misc.; Transporter, aquaporin family; Transporter; Membrane protein, multi-pass

Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; cell; cytoplasm; plasma membrane; integral to membrane; intercellular junction

Molecular Function: transporter activity; water channel activity; glycerol channel activity

Biological Process: water homeostasis; glycerol transport; cellular water homeostasis; sensory perception of sound; transport; water transport; cellular response to stress; transmembrane transport
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 Research Articles on AQP-4    1. This study demonistrated that Aquaporin-4 deletion in mice reduces brain disorder in experimental acute liver failure.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with AQP-4 elisa kitDiseases associated with AQP-4 elisa kit
 Products by Pathway  Pathway Diagram
 Aquaporin-mediated Transport Pathway antibodies  Aquaporin-mediated Transport Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Passive Transport By Aquaporins Pathway antibodies  Passive Transport By Aquaporins Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway antibodies  Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway Diagram
 Vasopressin-regulated Water Reabsorption Pathway antibodies  Vasopressin-regulated Water Reabsorption Pathway Diagram
 Vasopressin-regulated Water Reabsorption Pathway antibodies  Vasopressin-regulated Water Reabsorption Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >877 publications with AQP-4 and Nervous System Diseases
 Neuromyelitis Optica Antibodies  >521 publications with AQP-4 and Neuromyelitis Optica
 Brain Diseases Antibodies  >445 publications with AQP-4 and Brain Diseases
 Edema Antibodies  >439 publications with AQP-4 and Edema
 Brain Edema Antibodies  >375 publications with AQP-4 and Brain Edema
 Disease Models, Animal Antibodies  >195 publications with AQP-4 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >172 publications with AQP-4 and Cardiovascular Diseases
 Inflammation Antibodies  >118 publications with AQP-4 and Inflammation
 Necrosis Antibodies  >42 publications with AQP-4 and Necrosis
 Kidney Diseases Antibodies  >38 publications with AQP-4 and Kidney Diseases
Organs/Tissues associated with AQP-4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >921 publications with AQP-4 and Brain
 Spinal Cord Antibodies  >265 publications with AQP-4 and Spinal Cord
 Kidney Antibodies  >154 publications with AQP-4 and Kidney
 Eye Antibodies  >134 publications with AQP-4 and Eye
 Muscle Antibodies  >106 publications with AQP-4 and Muscle
 Lung Antibodies  >63 publications with AQP-4 and Lung
 Heart Antibodies  >36 publications with AQP-4 and Heart
 Intestine Antibodies  >27 publications with AQP-4 and Intestine
 Embryonic Tissue Antibodies  >25 publications with AQP-4 and Embryonic Tissue
 Thymus Antibodies  >5 publications with AQP-4 and Thymus
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