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ANA elisa kit :: Mouse Anti-nuclear ELISA Kit

Scan QR to view Datasheet Catalog #    MBS733339
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
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 Product Name   

Anti-nuclear (ANA), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Anti-nuclear Antibody ELISA Kit

 Product Synonym Names    Mouse Anti-nuclear Antibody ELISA Kit; Anti-nuclear; Anti-nuclear Antibody (Mouse)
 Product Gene Name   

ANA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 21; NC_000021.8 (18965968..18985268, complement). Location: 21q21.1
 OMIM    605674
 3D Structure    ModBase 3D Structure for Q14201
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ANAb. No significant cross-reactivity or interference between ANAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANAb and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive (Quantitative)
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ANA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ANA purchase    MBS733339 is a ready-to-use microwell, strip plate Competitive (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-nuclear (ANA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANA, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS733339 kit is based on ANA antibody-ANA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ANA elisa kit

   Intended Uses: This ANAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ANAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ANAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Nuclear antigen and an ANAb-HRP conjugate. The assay sample and buffer are incubated together with ANAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANAb concentration since ANAb from samples and ANAb-HRP conjugate compete for the Nuclear antigen binding site. Since the number of sites is limited, as more sites are occupied by ANAb from the sample, fewer sites are left to bind ANAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANAb concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ANA elisa kit    Mouse ELISA Kit
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 Typical Testing Data/Standard Curve (for reference only) of ANA elisa kit    ANA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS733339. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ANA. It may not necessarily be applicable to this product.
 NCBI GI #    3738222
 NCBI GeneID    10950
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 UniProt Primary Accession #    Q14201 [Other Products]
 UniProt Secondary Accession #    Q53XV1; Q96ET7; D3DSC4 [Other Products]
 UniProt Related Accession #    Q14201; Q6IAU3; Q9UHB2 [Other Products]
 Molecular Weight    29,116 Da
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 NCBI Official Full Name    ANA
 NCBI Official Synonym Full Names    BTG family, member 3
 NCBI Official Symbol    BTG3 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    protein BTG3; protein Tob5; OTTHUMP00000083229; OTTHUMP00000083230; B-cell translocation gene 3; abundant in neuroepithelium area protein
 UniProt Protein Name    Protein BTG3
 UniProt Synonym Protein Names   
Abundant in neuroepithelium area protein; BTG family member 3; Protein Tob5
 Protein Family    Protein anachronism
 UniProt Gene Name    BTG3 [Similar Products]
 UniProt Synonym Gene Names    ANA; TOB5 [Similar Products]
 UniProt Entry Name    BTG3_HUMAN
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 NCBI Summary for ANA    The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein might play a role in neurogenesis in the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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 UniProt Comments for ANA    BTG3: Overexpression impairs serum-induced cell cycle progression from the G0/G1 to S phase. Belongs to the BTG family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of cell proliferation; negative regulation of mitotic cell cycle
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 Research Articles on ANA    1. BTG3 is epigenetically silenced in renal cancer
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ANA elisa kitPathways associated with ANA elisa kit
 Reference Product  PubMed Publications
 CNOT7 elisa kit  >2 publications with ANA and CNOT7
 Products by Pathway  Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram
Diseases associated with ANA elisa kitOrgans/Tissues associated with ANA elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with ANA and Neoplasms
 Leukemia Antibodies  >3 publications with ANA and Leukemia
 Carcinoma Antibodies  >2 publications with ANA and Carcinoma
 Drug Toxicity Antibodies  >2 publications with ANA and Drug Toxicity
 Breast Neoplasms Antibodies  >1 publications with ANA and Breast Neoplasms
 Lung Neoplasms Antibodies  >1 publications with ANA and Lung Neoplasms
 Pain Antibodies  >1 publications with ANA and Pain
 Lymphoma Antibodies  >1 publications with ANA and Lymphoma
 Adenocarcinoma Antibodies  >1 publications with ANA and Adenocarcinoma
 Kidney Diseases Antibodies  >1 publications with ANA and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >3 publications with ANA and Nerve
 Brain Antibodies  >3 publications with ANA and Brain
 Kidney Antibodies  >2 publications with ANA and Kidney
 Lung Antibodies  >2 publications with ANA and Lung
 Muscle Antibodies  >2 publications with ANA and Muscle
 Connective Tissue Antibodies  >1 publications with ANA and Connective Tissue
 Blood Antibodies  >1 publications with ANA and Blood
 Ovary Antibodies  >1 publications with ANA and Ovary
 Prostate Antibodies  >1 publications with ANA and Prostate
 Heart Antibodies  >1 publications with ANA and Heart
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