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PR3 ab-IgG elisa kit :: Mouse Anti-proteinase 3 antibody IgG ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721278
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Anti-proteinase 3 antibody IgG (PR3 ab-IgG), ELISA Kit

 Also Known As   

Mouse Anti-proteinase 3 antibody IgG ELISA Kit

 Product Gene Name   

PR3 ab-IgG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Species Reactivity    Mouse
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL
 Intended Uses    This P- camkinase ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse P- camkinase. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of PR3 ab-IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PR3 ab-IgG purchase    MBS721278 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-proteinase 3 antibody IgG (PR3 ab-IgG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PR3 ab-IgG, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS721278 kit is based on PR3 ab-IgG antibody-PR3 ab-IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PR3 ab-IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PR3 ab-IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PR3 ab-IgG elisa kit

   Principle of the Assay: P- camkinase ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-P- camkinase antibody and an P- camkinase-HRP conjugate. The assay sample and buffer are incubated together with Pcamkinase- HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P- camkinase concentration since P- camkinase from samples and P- camkinase-HRP conjugate compete for the anti-P- camkinase antibody binding site. Since the number of sites is limited, as more sites are occupied by P- camkinase from the sample, fewer sites are left to bind P- camkinase-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P- camkinase concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for PR3 ab-IgG elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of PR3 ab-IgG elisa kit    PR3 ab-IgG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS721278
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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