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SLA elisa kit :: Mouse Anti-soluble liver antigen ELISA Kit

Scan QR to view Datasheet Catalog #    MBS726041
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Anti-soluble liver antigen (SLA), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Anti-soluble liver antigen ELISA Kit

 Product Gene Name   

SLA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 613811
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 Species Reactivity    Mouse
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    2.5-50u/mL
 Sensitivity    1.0u/mL
 Intended Uses    This ASLAg ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ASLAg. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SLA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SLA purchase    MBS726041 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-soluble liver antigen (SLA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50u/mL in biological research samples containing SLA, with an estimated sensitivity of 1.0u/mL. The ELISA analytical biochemical technique of the MBS726041 kit is based on SLA antibody-SLA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SLA elisa kit

   Principle of the Assay: ASLAg ELISA kit applies the competitive enzyme immunoassay technique utilizing soluble Liver Antigen and an ASLAg-HRP conjugate. The assay sample and buffer are incubated together with ASLAg-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASLAg concentration since ASLAg from samples and ASLAg-HRP conjugate compete for the soluble Liver Antigen binding site. Since the number of sites is limited, as more sites are occupied by ASLAg from the sample, fewer sites are left to bind ASLAg-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASLAg concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for SLA elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of SLA elisa kit    SLA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS726041. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SLA. It may not necessarily be applicable to this product.
 NCBI GI #    7652498
 NCBI GeneID    51091
 NCBI Accession #    AAD33963.2 [Other Products]
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 UniProt Secondary Accession #    Q0D2P3; Q17RT1; Q9NXZ5; Q9UGM9; Q9Y353; A8K8W1 [Other Products]
 UniProt Related Accession #    Q9HD40 [Other Products]
 Molecular Weight    55,411 Da
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 NCBI Official Full Name    soluble liver antigen/liver pancreas antigen
 NCBI Official Synonym Full Names    Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
 NCBI Official Symbol    SEPSECS [Similar Products]
 NCBI Official Synonym Symbols   
LP; SLA; PCH2D; SLA/LP
[Similar Products]
 NCBI Protein Information    O-phosphoseryl-tRNA(Sec) selenium transferase; O-phosphoseryl-tRNA(Sec) selenium transferase; SLA-p35; SLA/LP autoantigen; soluble liver antigen; liver-pancreas antigen; UGA suppressor tRNA-associated protein; tRNA(Ser/Sec)-associated antigenic protein; soluble liver antigen/liver pancreas antigen
 UniProt Protein Name    O-phosphoseryl-tRNA(Sec) selenium transferase
 UniProt Synonym Protein Names   
Liver-pancreas antigen; LP; SLA-p35; SLA/LP autoantigen; Selenocysteine synthase; Sec synthase; Selenocysteinyl-tRNA(Sec) synthase; Sep-tRNA:Sec-tRNA synthase; SepSecS; Soluble liver antigen; SLA; UGA suppressor tRNA-associated protein; tRNA(Ser/Sec)-associated antigenic protein
 Protein Family    S-layer protein
 UniProt Gene Name    SEPSECS [Similar Products]
 UniProt Synonym Gene Names    TRNP48; LP; Sec synthase; SepSecS; SLA [Similar Products]
 UniProt Entry Name    SPCS_HUMAN
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 NCBI Summary for SLA    The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011]
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 UniProt Comments for SLA    SLA/LP: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl- tRNA(Sec) required for selenoprotein biosynthesis. Defects in SEPSECS are the cause of pontocerebellar hypoplasia type 2D (PCH2D). PCH2D is a disorder characterized by postnatal onset of progressive atrophy of the cerebrum and cerebellum, microcephaly, profound mental retardation, spasticity, and variable seizures. Belongs to the SepSecS family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.9.1.2; RNA-binding; Transferase

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; transferase activity, transferring selenium-containing groups; tRNA binding; pyridoxal phosphate binding

Biological Process: selenocysteine incorporation

Disease: Pontocerebellar Hypoplasia, Type 2d
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 Research Articles on SLA    1. Data suggest SEPSECS silencing in placental trophoblasts inhibits proliferation, induces apoptosis, and reduces production of progesterone/chorionic gonadotropin (P/hCG); SEPSECS over-expression promotes cell proliferation and secretion of P/hCG.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SLA elisa kitPathways associated with SLA elisa kit
 Reference Product  PubMed Publications
 PSTK elisa kit  >7 publications with SLA and PSTK
 SELS elisa kit  >1 publications with SLA and SELS
 SEPHS1 elisa kit  >1 publications with SLA and SEPHS1
 Products by Pathway  Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Selenium Metabolism And Selenoproteins Pathway antibodies  Selenium Metabolism And Selenoproteins Pathway Diagram
 Selenocompound Metabolism Pathway antibodies  Selenocompound Metabolism Pathway Diagram
 Selenocompound Metabolism Pathway antibodies  Selenocompound Metabolism Pathway Diagram
Diseases associated with SLA elisa kitOrgans/Tissues associated with SLA elisa kit
 Disease Name  Pubmed Publications
 Hepatitis Antibodies  >1 publications with SLA and Hepatitis
 Liver Diseases Antibodies  >1 publications with SLA and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with SLA and Brain
 Placenta Antibodies  >1 publications with SLA and Placenta
 Liver Antibodies  >1 publications with SLA and Liver
 Blood Antibodies  >1 publications with SLA and Blood
 Muscle Antibodies  >1 publications with SLA and Muscle
 Embryonic Tissue Antibodies  >1 publications with SLA and Embryonic Tissue
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