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Pycard elisa kit :: Mouse Apoptosis-associated speck-like protein containing a CARD ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2885479
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Apoptosis-associated speck-like protein containing a CARD (Pycard), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Apoptosis-associated speck-like protein containing a CARD ELISA Kit

 Product Synonym Names    Apoptosis-associated speck-like protein containing a CARD; mASC; PYD and CARD domain-containing protein; Pycard; Asc
 Product Gene Name   

Pycard elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2885479 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9EPB4
 Species Reactivity    Mouse
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 Specificity    Recombinant and natural Mouse Apoptosis-associated speck-like protein containing a CARD,mASC
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 Assay Type    Sandwich
 Detection Range    78-5000 pg/mL
 Sensitivity    40.2 pg/mL
 Intra-Assay CV    <=4.1%
 Inter-Assay CV    <=6.7%
 Recovery    84%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Pycard elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Pycard purchase    MBS2885479 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apoptosis-associated speck-like protein containing a CARD (Pycard) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78-5000 pg/mL in biological research samples containing Pycard, with an estimated sensitivity of 40.2 pg/mL. The ELISA analytical biochemical technique of the MBS2885479 kit is based on Pycard antibody-Pycard antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Pycard antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Pycard. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Pycard elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Pycard elisa kit    Pycard elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2885479. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Pycard. It may not necessarily be applicable to this product.
 NCBI GI #    165377185
 NCBI GeneID    66824
 NCBI Accession #    NP_075747.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_023258.4 [Other Products]
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 UniProt Primary Accession #    Q9EPB4 [Other Products]
 UniProt Secondary Accession #    Q9D2W9 [Other Products]
 UniProt Related Accession #    Q9EPB4 [Other Products]
 Molecular Weight    21,459 Da
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 NCBI Official Full Name    apoptosis-associated speck-like protein containing a CARD
 NCBI Official Synonym Full Names    PYD and CARD domain containing
 NCBI Official Symbol    Pycard [Similar Products]
 NCBI Official Synonym Symbols   
Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik
[Similar Products]
 NCBI Protein Information    apoptosis-associated speck-like protein containing a CARD
 UniProt Protein Name    Apoptosis-associated speck-like protein containing a CARD
 UniProt Synonym Protein Names   
PYD and CARD domain-containing protein
 Protein Family    Apoptosis-associated speck-like protein
 UniProt Gene Name    Pycard [Similar Products]
 UniProt Synonym Gene Names    Asc; mASC [Similar Products]
 UniProt Entry Name    ASC_MOUSE
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 UniProt Comments for Pycard    PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Adaptor/scaffold

Cellular Component: cell soma; cytoplasm; cytosol; endoplasmic reticulum; extracellular region; IkappaB kinase complex; mitochondrion; nucleolus; nucleus; protein complex

Molecular Function: apoptotic protease activator activity; enzyme binding; identical protein binding; interleukin-6 receptor binding; myosin I binding; protease binding; protein binding; protein dimerization activity; protein homodimerization activity; Pyrin domain binding; tropomyosin binding

Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; apoptosis; caspase activation; defense response to Gram-negative bacterium; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; immune system process; inflammatory response; inhibition of NF-kappaB transcription factor; innate immune response; interleukin-1 beta production; myeloid dendritic cell activation; myeloid dendritic cell activation during immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interferon-beta production; positive regulation of actin filament polymerization; positive regulation of activated T cell proliferation; positive regulation of adaptive immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of defense response to virus by host; positive regulation of interferon-gamma production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of phagocytosis; positive regulation of T cell activation; positive regulation of transcription factor activity; positive regulation of tumor necrosis factor production; regulation of apoptosis; regulation of autophagy; regulation of caspase activity; regulation of GTPase activity; regulation of inflammatory response; regulation of protein stability; response to bacterium; tumor necrosis factor-mediated signaling pathway
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 Research Articles on Pycard    1. These data identify a novel non-canonical immunoregulatory function of NLRP3 and ASC in autoimmunity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Pycard elisa kitPathways associated with Pycard elisa kit
 Reference Product  PubMed Publications
 Casp1 elisa kit  >264 publications with Pycard and Casp1
 Nlrp3 elisa kit  >192 publications with Pycard and Nlrp3
 Il18 elisa kit  >60 publications with Pycard and Il18
 Nlrc4 elisa kit  >32 publications with Pycard and Nlrc4
 Aim2 elisa kit  >30 publications with Pycard and Aim2
 Il1b elisa kit  >18 publications with Pycard and Il1b
 Mefv elisa kit  >8 publications with Pycard and Mefv
 Nlrp1b elisa kit  >2 publications with Pycard and Nlrp1b
 Products by Pathway  Pathway Diagram
 C-type Lectin Receptors (CLRs) Pathway antibodies  C-type Lectin Receptors (CLRs) Pathway Diagram
 CLEC7A (Dectin-1) Signaling Pathway antibodies  CLEC7A (Dectin-1) Signaling Pathway Diagram
 CLEC7A/inflammasome Pathway antibodies  CLEC7A/inflammasome Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammasomes Pathway antibodies  Inflammasomes Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
Diseases associated with Pycard elisa kitOrgans/Tissues associated with Pycard elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >179 publications with Pycard and Inflammation
 Necrosis Antibodies  >62 publications with Pycard and Necrosis
 Disease Models, Animal Antibodies  >39 publications with Pycard and Disease Models, Animal
 Nervous System Diseases Antibodies  >24 publications with Pycard and Nervous System Diseases
 Brain Diseases Antibodies  >21 publications with Pycard and Brain Diseases
 Cardiovascular Diseases Antibodies  >20 publications with Pycard and Cardiovascular Diseases
 Kidney Diseases Antibodies  >16 publications with Pycard and Kidney Diseases
 Adenocarcinoma Antibodies  >14 publications with Pycard and Adenocarcinoma
 Atherosclerosis Antibodies  >12 publications with Pycard and Atherosclerosis
 Fibrosis Antibodies  >12 publications with Pycard and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >48 publications with Pycard and Bone
 Bone Marrow Antibodies  >39 publications with Pycard and Bone Marrow
 Brain Antibodies  >31 publications with Pycard and Brain
 Kidney Antibodies  >28 publications with Pycard and Kidney
 Lung Antibodies  >26 publications with Pycard and Lung
 Intestine Antibodies  >14 publications with Pycard and Intestine
 Heart Antibodies  >11 publications with Pycard and Heart
 Joint Antibodies  >11 publications with Pycard and Joint
 Spleen Antibodies  >7 publications with Pycard and Spleen
 Pancreas Antibodies  >6 publications with Pycard and Pancreas
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